PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID PGSC0003DMP400000652
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum
Family bHLH
Protein Properties Length: 700aa    MW: 79315 Da    PI: 8.5379
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
PGSC0003DMP400000652genomePGSCView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH37.83.5e-12536581454
                           HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
                   HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                            h + ErrRR+++N+ f+ Lr+llP+       K +Ka++L  ++eY++sL
  PGSC0003DMP400000652 536 HHMISERRRREKLNENFQHLRSLLPPE-----IKKDKASVLASTTEYLSSL 581
                           68899*********************8.....699**************99 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:3.30.43.101.1E-625105IPR016167FAD-binding, type 2, subdomain 1
SuperFamilySSF561761.96E-3528226IPR016166FAD-binding, type 2
PROSITE profilePS5138713.38746229IPR016166FAD-binding, type 2
PROSITE patternPS0086205085IPR006093Oxygen oxidoreductase covalent FAD-binding site
PfamPF015652.0E-1650198IPR006094FAD linked oxidase, N-terminal
Gene3DG3DSA:3.30.465.101.2E-8142225IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
PfamPF092652.3E-68230409IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domain
SuperFamilySSF551036.02E-45230408IPR016164FAD-linked oxidase-like, C-terminal
Gene3DG3DSA:3.40.462.101.8E-71232411IPR016170Vanillyl-alcohol oxidase/Cytokinin dehydrogenase C-terminal domain
PROSITE profilePS5088814.557532581IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000838.46E-12532586No hitNo description
SuperFamilySSF474591.44E-14534598IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.103.3E-14535598IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.2E-9536581IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003539.0E-12538587IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009690Biological Processcytokinin metabolic process
GO:0055114Biological Processoxidation-reduction process
GO:0016614Molecular Functionoxidoreductase activity, acting on CH-OH group of donors
GO:0019139Molecular Functioncytokinin dehydrogenase activity
GO:0046983Molecular Functionprotein dimerization activity
GO:0050660Molecular Functionflavin adenine dinucleotide binding
Sequence ? help Back to Top
Protein Sequence    Length: 700 aa     Download sequence    Send to blast
MSNLGKLKSW NPSIPQEIFS LNISSKLSTN SDDIKESSKD FGKIVQKKFP AAVLYPSCVN  60
DIIDLIQFSY DLSVPFQVAA KGHGHSIRGQ AMAQNGVIVE MNSLNNNNNE NCGVRVSWDL  120
DLGFYADVGG EQLWIDVLRS TLEYGLAPVS WTDYLYLTVG GTLSNAGISG QTFRYGPQIS  180
NVHEMDVITG KGELVTCSKD KNSELFFGVL GGLGQFGIII RAKIVLDKAP TRVKWVRMLY  240
DDFPKFTKDQ EYLISIHHNG LDYVEGSLMM EQSSLNNWRS SFFSPSNQAK IVSLLSQNKI  300
MYCLEMVKYY DDHTANTIDE ELKKLVQGLN YLGGFMFKKD VTFVEFLNRV RKGQIELELK  360
GMWDVPHPWL NLFVPKSNIM QFNAAVFEDI ILRQNKPTGP ILVYPTSRKS EYSSLFHNVA  420
KTCYVTQENQ TLTQFPKIEG EDDVIRQAYL AVISSSSPSS SSRGQIQEIL TRDHRLVTRN  480
TTAFTRFRST NYASIGATSS CRQNMLKRSI TFFKNLYTMN RQEGIQVNRA MSTQVHHMIS  540
ERRRREKLNE NFQHLRSLLP PEIKKDKASV LASTTEYLSS LKDQVEKLCK RNEILEATLL  600
SKKEVSQFQQ NGSGRVYIYI TNIEERIVDL QVIAKGKCNT LDLVICLMEF LKMASYVNLM  660
AIDANTTMVQ SCPLTQITLR LRIQVIYVSH SVFDICIGD*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4o95_A1e-1382840827412Cytokinin dehydrogenase 4
4oal_A1e-1382840827412Cytokinin dehydrogenase 4
4oal_B1e-1382840827412Cytokinin dehydrogenase 4
5hhz_A1e-1382840827412Cytokinin dehydrogenase 4
5hmr_A1e-1382840827412Cytokinin dehydrogenase 4
5hqx_A1e-1382840827412Cytokinin dehydrogenase 4
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1540545ERRRRE
2541546RRRREK
Functional Description ? help Back to Top
Source Description
UniProtCatalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. {ECO:0000269|PubMed:14555694}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapPGSC0003DMP400000652
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Regulated by GATA18/HAN. {ECO:0000269|PubMed:26390296}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankFJ7512390.0FJ751239.1 Solanum tuberosum cytokinin oxidase/dehydrogenase 2 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_027769668.10.0cytokinin dehydrogenase 3-like
SwissprotQ9LTS31e-156CKX3_ARATH; Cytokinin dehydrogenase 3
TrEMBLM0ZHH70.0M0ZHH7_SOLTU; Uncharacterized protein
STRINGPGSC0003DMT4000008830.0(Solanum tuberosum)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G56960.16e-30bHLH family protein
Publications ? help Back to Top
  1. Bilyeu KD, et al.
    Molecular and biochemical characterization of a cytokinin oxidase from maize.
    Plant Physiol., 2001. 125(1): p. 378-86
    [PMID:11154345]
  2. Werner T,Motyka V,Strnad M,Schmülling T
    Regulation of plant growth by cytokinin.
    Proc. Natl. Acad. Sci. U.S.A., 2001. 98(18): p. 10487-92
    [PMID:11504909]
  3. Schmülling T,Werner T,Riefler M,Krupková E,Bartrina y Manns I
    Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species.
    J. Plant Res., 2003. 116(3): p. 241-52
    [PMID:12721786]
  4. Werner T, et al.
    Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.
    Plant Cell, 2003. 15(11): p. 2532-50
    [PMID:14555694]
  5. Lohar DP, et al.
    Cytokinins play opposite roles in lateral root formation, and nematode and Rhizobial symbioses.
    Plant J., 2004. 38(2): p. 203-14
    [PMID:15078325]
  6. Deeken R, et al.
    An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach.
    Plant Cell, 2006. 18(12): p. 3617-34
    [PMID:17172353]
  7. Kowalska M, et al.
    Vacuolar and cytosolic cytokinin dehydrogenases of Arabidopsis thaliana: heterologous expression, purification and properties.
    Phytochemistry, 2010. 71(17-18): p. 1970-8
    [PMID:20825956]
  8. Gajdosová S, et al.
    Distribution, biological activities, metabolism, and the conceivable function of cis-zeatin-type cytokinins in plants.
    J. Exp. Bot., 2011. 62(8): p. 2827-40
    [PMID:21282330]
  9. Xu X, et al.
    Genome sequence and analysis of the tuber crop potato.
    Nature, 2011. 475(7355): p. 189-95
    [PMID:21743474]
  10. Xiao D, et al.
    SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis.
    Plant Physiol., 2015. 169(2): p. 1275-91
    [PMID:26304848]
  11. Han Y,Jiao Y
    APETALA1 establishes determinate floral meristem through regulating cytokinins homeostasis in Arabidopsis.
    Plant Signal Behav, 2015. 10(11): p. e989039
    [PMID:26359644]
  12. Pernisova M, et al.
    Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis.
    New Phytol., 2016. 212(2): p. 497-509
    [PMID:27322763]
  13. Farber M,Attia Z,Weiss D
    Cytokinin activity increases stomatal density and transpiration rate in tomato.
    J. Exp. Bot., 2016. 67(22): p. 6351-6362
    [PMID:27811005]