PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID 676766060
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium
Family ERF
Protein Properties Length: 833aa    MW: 94429.9 Da    PI: 5.3394
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
676766060genomeVEGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP259.39e-19654703255
        AP2   2 gykGVrwdkkrgrWvAeIrdpsengkr.krfslgkfgtaeeAakaaiaarkkleg 55 
                +y+GVr+++ +g+W+AeIrdp+    + +r++lg+f taeeAa a+++a++k++g
  676766060 654 HYRGVRQRP-WGKWAAEIRDPK----KaARVWLGTFETAEEAAFAYDRAALKFKG 703
                7********.**********94....45************************987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF033213.4E-19715554IPR004993GH3 family
Gene3DG3DSA:3.30.730.105.1E-32653712IPR001471AP2/ERF domain
SuperFamilySSF541716.54E-22654712IPR016177DNA-binding domain
PfamPF008474.7E-12654703IPR001471AP2/ERF domain
SMARTSM003806.3E-38654717IPR001471AP2/ERF domain
CDDcd000184.19E-33654713No hitNo description
PROSITE profilePS5103224.869654711IPR001471AP2/ERF domain
PRINTSPR003677.5E-11655666IPR001471AP2/ERF domain
PRINTSPR003677.5E-11677693IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009816Biological Processdefense response to bacterium, incompatible interaction
GO:0009863Biological Processsalicylic acid mediated signaling pathway
GO:0010112Biological Processregulation of systemic acquired resistance
GO:0016046Biological Processdetection of fungus
GO:0018874Biological Processbenzoate metabolic process
GO:0034052Biological Processpositive regulation of plant-type hypersensitive response
GO:0044419Biological Processinterspecies interaction between organisms
GO:0071456Biological Processcellular response to hypoxia
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0052625Molecular Function4-aminobenzoate amino acid synthetase activity
GO:0052626Molecular Functionbenzoate amino acid synthetase activity
GO:0052627Molecular Functionvanillate amino acid synthetase activity
GO:0052628Molecular Function4-hydroxybenzoate amino acid synthetase activity
Sequence ? help Back to Top
Protein Sequence    Length: 833 aa     Download sequence    Send to blast
MKPISNSNDI FEAKLEDLTS NPQQIQDNLL EEIVTPNLKT EYLQRFHMVR FDKELFKKNV  60
PIVSYEDIKP YIDRVVNGES SDVISARQIT GFLLSSGTSG GAQKMMPWNN KYLDNLTFAY  120
DLRKHIVTKH VKGLEEGKGM MFLFTKQESI TPSGLPARVA TSSYFKSDYF KNRPSNWYYS  180
YTSPDEVILC SNNTQSLYCH LLCGLLQRDE VVRMGSIFAS VMVRAIKFLE TYWEELCSNI  240
RSGHLSEWIT DLGCRDSVSL ALGGPRPDLA DTIKTICNQN SWKGIVKRLW PKTKYIETVV  300
TGSMGQYVPT LNYYCSDLPL VSTTYGSSET TFGINVNPLC KPEDVSYAFM PNMSYFEFIT  360
MDGEERNVVD LQDVKVGCTY EPVVTNFAGL YRMRVGDILL VTGFYNNAPE FKFVRRENVV  420
LSIDSDKTNE EDLFKAVEQV KLVLDSSDLI LVEYTSYADT STFPGHYVVY VEAKAKDGEK  480
NMPSEVNEEA FSKCCSVMED SFDNVYKRCR FKDGSVGPLE IRVVEQGTFD SLMDFFISQG  540
ASISQYKTPR CIKSGKALEF MEGYVVASVV FNSARETNKE VDRRYGKEPV PPEASAAENP  600
SWNESDVTAM ISSLSRAIEY PAADGHERSD PPVKQELDKS DQQQQDQDQP RRRHYRGVRQ  660
RPWGKWAAEI RDPKKAARVW LGTFETAEEA AFAYDRAALK FKGTKAKLNF PEHVQGPSAT  720
TYLASQSGID HAAKGGSELM SSPPPRLGPS TTTTTWPTNY NQDILQYAQL LTSNNDVDLS  780
YYTSSLFSQQ QQPFSTPSSS SSSSLTSQQQ HQHQHQQREE ENYGYHYYNY PRE
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4epm_A0.0156775764-substituted benzoates-glutamate ligase GH3.12
4eq4_A0.0156775764-substituted benzoates-glutamate ligase GH3.12
4eq4_B0.0156775764-substituted benzoates-glutamate ligase GH3.12
4eql_A0.0156775764-substituted benzoates-glutamate ligase GH3.12
4eql_B0.0156775764-substituted benzoates-glutamate ligase GH3.12
4ewv_A0.0156775764-substituted benzoates-glutamate ligase GH3.12
4ewv_B0.0156775764-substituted benzoates-glutamate ligase GH3.12
4l39_A0.0156775764-substituted benzoates-glutamate ligase GH3.12
4l39_B0.0156775764-substituted benzoates-glutamate ligase GH3.12
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtCatalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR). {ECO:0000269|PubMed:10224270, ECO:0000269|PubMed:11846877, ECO:0000269|PubMed:16353557, ECO:0000269|PubMed:17468220, ECO:0000269|PubMed:17521413, ECO:0000269|PubMed:17918621, ECO:0000269|PubMed:18266921, ECO:0000269|PubMed:19189963}.
Cis-element ? help Back to Top
SourceLink
PlantRegMap676766060
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By P.syringae pv. maculicola and pv. tomato. Induced by PAD4 locally at the infection site and in a salicylic acid-dependent manner systemically. {ECO:0000269|PubMed:17468220, ECO:0000269|PubMed:17521413, ECO:0000269|PubMed:17918621, ECO:0000269|PubMed:18266921}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013620838.10.0PREDICTED: 4-substituted benzoates-glutamate ligase GH3.12-like
SwissprotQ9LYU40.0GH312_ARATH; 4-substituted benzoates-glutamate ligase GH3.12
TrEMBLA0A3N6QY700.0A0A3N6QY70_BRACR; Uncharacterized protein
STRINGBo2g011190.10.0(Brassica oleracea)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM554322
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G13330.18e-56related to AP2 6l
Publications ? help Back to Top
  1. Warren RF,Merritt PM,Holub E,Innes RW
    Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis.
    Genetics, 1999. 152(1): p. 401-12
    [PMID:10224270]
  2. van der Biezen EA,Freddie CT,Kahn K,Parker JE,Jones JD
    Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-LRR genes and confers downy mildew resistance through multiple signalling components.
    Plant J., 2002. 29(4): p. 439-51
    [PMID:11846877]
  3. Xiao S, et al.
    The atypical resistance gene, RPW8, recruits components of basal defence for powdery mildew resistance in Arabidopsis.
    Plant J., 2005. 42(1): p. 95-110
    [PMID:15773856]
  4. McDowell JM,Williams SG,Funderburg NT,Eulgem T,Dangl JL
    Genetic analysis of developmentally regulated resistance to downy mildew (Hyaloperonospora parasitica) in Arabidopsis thaliana.
    Mol. Plant Microbe Interact., 2005. 18(11): p. 1226-34
    [PMID:16353557]
  5. Nobuta K, et al.
    The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis.
    Plant Physiol., 2007. 144(2): p. 1144-56
    [PMID:17468220]
  6. Jagadeeswaran G, et al.
    Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae.
    Plant J., 2007. 51(2): p. 234-46
    [PMID:17521413]
  7. Lee MW,Lu H,Jung HW,Greenberg JT
    A key role for the Arabidopsis WIN3 protein in disease resistance triggered by Pseudomonas syringae that secrete AvrRpt2.
    Mol. Plant Microbe Interact., 2007. 20(10): p. 1192-200
    [PMID:17918621]
  8. Wang L, et al.
    The genetic network controlling the Arabidopsis transcriptional response to Pseudomonas syringae pv. maculicola: roles of major regulators and the phytotoxin coronatine.
    Mol. Plant Microbe Interact., 2008. 21(11): p. 1408-20
    [PMID:18842091]
  9. Okrent RA,Brooks MD,Wildermuth MC
    Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.
    J. Biol. Chem., 2009. 284(15): p. 9742-54
    [PMID:19189963]
  10. Chen Z,Zheng Z,Huang J,Lai Z,Fan B
    Biosynthesis of salicylic acid in plants.
    Plant Signal Behav, 2009. 4(6): p. 493-6
    [PMID:19816125]
  11. Okrent RA,Wildermuth MC
    Evolutionary history of the GH3 family of acyl adenylases in rosids.
    Plant Mol. Biol., 2011. 76(6): p. 489-505
    [PMID:21594748]
  12. Westfall CS, et al.
    Structural basis for prereceptor modulation of plant hormones by GH3 proteins.
    Science, 2012. 336(6089): p. 1708-11
    [PMID:22628555]
  13. Round A, et al.
    Determination of the GH3.12 protein conformation through HPLC-integrated SAXS measurements combined with X-ray crystallography.
    Acta Crystallogr. D Biol. Crystallogr., 2013. 69(Pt 10): p. 2072-80
    [PMID:24100325]
  14. Mackelprang R,Okrent RA,Wildermuth MC
    Preference of Arabidopsis thaliana GH3.5 acyl amido synthetase for growth versus defense hormone acyl substrates is dictated by concentration of amino acid substrate aspartate.
    Phytochemistry, 2017. 143: p. 19-28
    [PMID:28743075]