PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Rsa1.0_06227.1_g00001.1
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus
Family WRKY
Protein Properties Length: 329aa    MW: 36759.4 Da    PI: 5.8725
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Rsa1.0_06227.1_g00001.1genomeRGDView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
                              T-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS
                     WRKY  17 gsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                              g  + +sY+rCt++   gC+++k+v++ +++p+++ itY g+H+++
                              566789*******99999**************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM007742.3E-1188138IPR003657WRKY domain
Gene3DG3DSA: domain
PROSITE profilePS5081112.11791139IPR003657WRKY domain
SuperFamilySSF1182901.27E-1191138IPR003657WRKY domain
PfamPF031064.8E-1292136IPR003657WRKY domain
SuperFamilySSF511613.98E-10254307IPR011004Trimeric LpxA-like
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 329 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1yp2_A8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp2_B8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp2_C8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp2_D8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp3_A8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp3_B8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp3_C8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp3_D8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp4_A8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp4_B8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp4_C8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
1yp4_D8e-17234309369444Glucose-1-phosphate adenylyltransferase small subunit
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways (PubMed:14742872, PubMed:16623907, PubMed:17310369, PubMed:28576847). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression (PubMed:24104863). Plays an important but not indispensable role in jasmonate and salicylic acid signaling (PubMed:18713432). Regulates positively the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways (PubMed:14742872, PubMed:16623907, PubMed:18713432, PubMed:28837631). Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways (PubMed:28837631, PubMed:16623907, PubMed:22325892). Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2) (PubMed:22325892, PubMed:16623907). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS) (PubMed:16623907). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression (PubMed:17310369). Negative regulator of SA biosynthesis (PubMed:28837631). Negative regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1) (PubMed:17313163). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae) (PubMed:25339349). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:17310369, PubMed:22268143). Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000 (PubMed:26433201). {ECO:0000250|UniProtKB:Q9SUP6, ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:16623907, ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17313163, ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:24104863, ECO:0000269|PubMed:25339349, ECO:0000269|PubMed:26433201, ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631, ECO:0000303|PubMed:28837631}.
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Regulated by MYB44 (PubMed:23067202, PubMed:23603962). Basal expression levels require the presence of endogeneous salicylic acid (SA) (PubMed:14742872, PubMed:17310369). Induced by reactive oxygen species (ROS) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Induced by SA; early induction is NPR1-independent, but full-scale induction is NPR1-dependent (PubMed:14742872, PubMed:22325892, PubMed:22268143, PubMed:26433201). Up-regulated by benzothiadiazole (BTH) (PubMed:26433201). Repressed by jasmonic acid (MeJA) by both COI1-dependent and COI1-independent pathways (PubMed:14742872, PubMed:18713432). Triggered by the pathogenic compatible bacteria E.carotovora subsp. carotovora SCC3193 (PubMed:14742872). Induced by P.syringae pv. tomato DC3000 (PubMed:17965588, PubMed:22325892). Stimulated by ATX1 (PubMed:17965588). Up-regulated by E.amylovora (PubMed:22316300). Accumulates during leaf and flower senescence (PubMed:17310369). Induced expression upon simultaneous feeding by caterpillars (e.g. P.xylostella) and aphids (e.g. B.brassicae) at a low density, but lower levels in plants induced with both caterpillars and a high aphid density (PubMed:25339349). Responsive to rhizobacterium B.cereus AR156 in leaves (PubMed:26433201). {ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17965588, ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:23067202, ECO:0000269|PubMed:23603962, ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:25339349, ECO:0000269|PubMed:26433201}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankKF4300761e-171KF430076.1 Brassica rapa WRKY transcription factor 70 (WRKY70) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_018442148.11e-165PREDICTED: probable WRKY transcription factor 70
SwissprotQ9LY001e-101WRK70_ARATH; Probable WRKY transcription factor 70
TrEMBLV5RF081e-133V5RF08_BRACM; WRKY transcription factor 70
STRINGBra003239.1-P1e-133(Brassica rapa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G56400.12e-89WRKY DNA-binding protein 70
Publications ? help Back to Top
  1. Zhang X, et al.
    Arabidopsis cysteine-rich receptor-like kinase 45 positively regulates disease resistance to Pseudomonas syringae.
    Plant Physiol. Biochem., 2013. 73: p. 383-91
  2. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  3. Brosché M, et al.
    Transcriptomics and functional genomics of ROS-induced cell death regulation by RADICAL-INDUCED CELL DEATH1.
    PLoS Genet., 2014. 10(2): p. e1004112
  4. Bao F, et al.
    Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses.
    New Phytol., 2014. 202(4): p. 1320-34
  5. Wang F, et al.
    TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.
    Front Plant Sci, 2015. 6: p. 108
  6. Jiang CH, et al.
    Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.
    J. Exp. Bot., 2016. 67(1): p. 157-74
  7. Kaurilind E,Xu E,Brosché M
    A genetic framework for H2O2 induced cell death in Arabidopsis thaliana.
    BMC Genomics, 2015. 16: p. 837
  8. Rodriguez-Salus M, et al.
    The Synthetic Elicitor 2-(5-Bromo-2-Hydroxy-Phenyl)-Thiazolidine-4-Carboxylic Acid Links Plant Immunity to Hormesis.
    Plant Physiol., 2016. 170(1): p. 444-58
  9. Onkokesung N,Reichelt M,van Doorn A,Schuurink RC,Dicke M
    Differential Costs of Two Distinct Resistance Mechanisms Induced by Different Herbivore Species in Arabidopsis.
    Plant Physiol., 2016. 170(2): p. 891-906
  10. Chen J, et al.
    Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses.
    Plant Cell, 2017. 29(6): p. 1425-1439
  11. Li J,Zhong R,Palva ET
    WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis.
    PLoS ONE, 2017. 12(8): p. e0183731
  12. Zhou M, et al.
    WRKY70 prevents axenic activation of plant immunity by direct repression of SARD1.
    New Phytol., 2018. 217(2): p. 700-712