PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID LOC_Os02g02820.1
Common NameBHLH5, LOC4328113, OJ1020_C02.6, Os02g0120500, OsJ_05158, OSJNBb0088N06.15, TDR
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa
Family bHLH
Protein Properties Length: 553aa    MW: 58274.4 Da    PI: 4.4495
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
LOC_Os02g02820.1genomeMSUView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH36.96.6e-12288331755
                       HHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
               HLH   7 erErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                       e+Er+RR+++N  + +Lr+l+P+      +K++ a+iL  A++YI  Lq
  LOC_Os02g02820.1 288 EAERKRRKKLNGHLYKLRSLVPNI-----TKMDRASILGDAIDYIVGLQ 331
                       89*********************7.....7***************9887 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000831.18E-13280342No hitNo description
PROSITE profilePS5088815.37281330IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474598.51E-18282353IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003531.2E-15287336IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.4E-9288331IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.101.1E-14288343IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009555Biological Processpollen development
GO:0048657Biological Processanther wall tapetum cell differentiation
GO:0005634Cellular Componentnucleus
GO:0000978Molecular FunctionRNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0003677Molecular FunctionDNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009066anatomyanther
PO:0001004developmental stageanther development stage
PO:0007130developmental stagesporophyte reproductive stage
Sequence ? help Back to Top
Protein Sequence    Length: 553 aa     Download sequence    Send to blast
MGRGDHLLMK NSNAAAAAAA VNGGGTSLDA ALRPLVGSDG WDYCIYWRLS PDQRFLEMTG  60
FCCSSELEAQ VSALLDLPSS IPLDSSSIGM HAQALLSNQP IWQSSSEEEE ADGGGGAKTR  120
LLVPVAGGLV ELFASRYMAE EQQMAELVMA QCGGGGAGDD GGGQAWPPPE TPSFQWDGGA  180
DAQRLMYGGS SLNLFDAAAA DDDPFLGGGG GDAVGDEAAA AGAWPYAGMA VSEPSVAVAQ  240
EQMQHAAGGG VAESGSEGRK LHGGDPEDDG DGEGRSGGAK RQQCKNLEAE RKRRKKLNGH  300
LYKLRSLVPN ITKMDRASIL GDAIDYIVGL QKQVKELQDE LEDNHVHHKP PDVLIDHPPP  360
ASLVGLDNDD ASPPNSHQQQ PPLAVSGSSS RRSNKDPAMT DDKVGGGGGG GHRMEPQLEV  420
RQVQGNELFV QVLWEHKPGG FVRLMDAMNA LGLEVINVNV TTYKTLVLNV FRVMVRDSEV  480
AVQADRVRDS LLEVTRETYP GVWPSPQEED DAKFDGGDGG QAAAAAAAAG GEHYHDEVGG  540
GYHQHLHYLA FD*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1289296ERKRRKKL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Os.500000.0flower| panicle
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO379892030.0
Expression AtlasQ6YUS3-
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in the regulation of tapetum programmed cell death (PCD) and degradation during male reproductive development. Promotes tapetal PCD. Positively regulates the expression of two tapetum-specific genes, the cysteine protease CP1 and the lipid-transfer protein C6 (PubMed:17138695). Acts upstream from and interacts with EAT1/DTD in the regulation of tapetal PCD (PubMed:23385589). Regulates the expression of genes related to aliphatic metabolism during pollen development. May play regulatory role in the lipidic metabolism involved in the formation of pollen wall (PubMed:19825565). {ECO:0000269|PubMed:17138695, ECO:0000269|PubMed:19825565, ECO:0000269|PubMed:23385589}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapLOC_Os02g02820.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Male sterility due to failure of tapetum programmed cell death (PCD) and degeneration, and collapse of microspores in developing anthers (PubMed:17138695). Altered pollen wall formation (PubMed:19825565). {ECO:0000269|PubMed:17138695, ECO:0000269|PubMed:19825565}.
Phenotype -- Mutation ? help Back to Top
Source ID
RiceGEOs02g02820
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK1195800.0AK119580.1 Oryza sativa Japonica Group cDNA clone:002-117-B12, full insert sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_015625730.10.0PREDICTED: transcription factor ABORTED MICROSPORES
SwissprotQ6YUS30.0TDR_ORYSJ; Transcription factor TDR
TrEMBLA0A0E0N8Z70.0A0A0E0N8Z7_ORYRU; Uncharacterized protein
STRINGLOC_Os02g02820.10.0(Oryza sativa Japonica Group)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP37041323
MonocotsOGMP55303449
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G16910.14e-50bHLH family protein
Publications ? help Back to Top
  1. Li X, et al.
    Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis.
    Plant Physiol., 2006. 141(4): p. 1167-84
    [PMID:16896230]
  2. Li N, et al.
    The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development.
    Plant Cell, 2006. 18(11): p. 2999-3014
    [PMID:17138695]
  3. Zhang DS, et al.
    Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development.
    Mol Plant, 2008. 1(4): p. 599-610
    [PMID:19825565]
  4. Niu N, et al.
    EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice.
    Nat Commun, 2013. 4: p. 1445
    [PMID:23385589]
  5. Ji C, et al.
    A novel rice bHLH transcription factor, DTD, acts coordinately with TDR in controlling tapetum function and pollen development.
    Mol Plant, 2013. 6(5): p. 1715-8
    [PMID:23519457]
  6. Ko SS, et al.
    The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice.
    Plant Cell, 2014. 26(6): p. 2486-2504
    [PMID:24894043]