PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID LOC_Os01g18870.1
Common NameBHLH142, LOC4325164, Os01g0293100, P0706B05.43, TIP2
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa
Family bHLH
Protein Properties Length: 380aa    MW: 40706 Da    PI: 6.6533
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
LOC_Os01g18870.1genomeMSUView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH22.22.5e-07180222754
                       HHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
               HLH   7 erErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                       + E++RR r+ ++++ L  l+P++     +K + a++   A+eYI++L
  LOC_Os01g18870.1 180 KKEKQRRLRLTEKYNALMLLIPNR-----TKEDRATVISDAIEYIQEL 222
                       57*********************9.....8****************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088810.264173222IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.9E-4179228IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000104.9E-5180222IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474593.53E-9182243IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.108.6E-8182246IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.14E-5182222No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009066anatomyanther
PO:0001004developmental stageanther development stage
PO:0007130developmental stagesporophyte reproductive stage
Sequence ? help Back to Top
Protein Sequence    Length: 380 aa     Download sequence    Send to blast
MYHPQCELLM PLESLEMDVG QSHLAAAVAA AMPGELNFHL LHSLDAAAAA ASSTAASASS  60
QPTVDYFFGG ADQQPPPPAA MQYDQLAAPH HHQTVAMLRD YYGGHYPPAA AAAAATEAYF  120
RGGPRTAGSS SLVFGPADDE SAFMVGPFES SPTPRSGGGR KRSRATAGFH GGGPANGVEK  180
KEKQRRLRLT EKYNALMLLI PNRTKEDRAT VISDAIEYIQ ELGRTVEELT LLVEKKRRRR  240
EMQGDVVDAA TSSVVAGMDQ AAESSEGEVM AAAAMGAVAP PPRQAPIRST YIQRRSKETF  300
VDVRIVEDDV NIKLTKRRRD GCLAAASRAL DDLRLDLVHL SGGKIGDCHI YMFNTKIHSG  360
SPVFASAVAS RLIEVVDEY*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1158164GRKRSRA
2234239KKRRRR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Os.548280.0panicle
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO329920590.0
Expression AtlasQ5JNS0-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: In anthers, expressed from the meiosis stage to mitosis, with maximum levels at stages 7 and 8, when meiosis initiates, and when endothecium and the middle layer become condensed, respectively.
UniprotTISSUE SPECIFICITY: Highly expressed in anthers; strong expression in the middle layer and tapetum, and weak expression in the endothecium. {ECO:0000269|PubMed:24755456}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor that binds to the E-box-containing promoter regions of the transcription factors TDR and EAT1, activating their expression. May have a role in specifying the cell pattern of the inner anther walls and functioning in meiosis progression. Required for male reproduction (PubMed:24755456). Acts downstream of UDT1 and GAMYB, but upstream of TDR1 and EAT1 in pollen development (PubMed:24894043). {ECO:0000269|PubMed:24755456, ECO:0000269|PubMed:24894043}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapLOC_Os01g18870.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Has normal vegetative growth, inflorescence and flower morphology, however has smaller anthers and does not produce mature pollen grains during reproductive stage. After stage 6, during which anther forms four somatic layers, the three inner anther wall layers remain undifferentiated. Meiosis progresses normally from leptotene stage to metaphase I, but is arrasted at anaphase I and no cytokinesis occurs at prophase II, resulting in degenerated meiocytes. {ECO:0000269|PubMed:24755456}.
Phenotype -- Mutation ? help Back to Top
Source ID
RiceGEOs01g18870
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK1068500.0AK106850.1 Oryza sativa Japonica Group cDNA clone:002-118-A11, full insert sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_015645943.10.0PREDICTED: transcription factor TIP2
SwissprotQ5JNS00.0TIP2_ORYSJ; Transcription factor TIP2
TrEMBLA0A0E0MV070.0A0A0E0MV07_ORYRU; Uncharacterized protein
STRINGLOC_Os01g18870.10.0(Oryza sativa Japonica Group)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP26591431
MonocotsOGMP19043796
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G31210.18e-35bHLH family protein
Publications ? help Back to Top
  1. Li X, et al.
    Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis.
    Plant Physiol., 2006. 141(4): p. 1167-84
    [PMID:16896230]
  2. Fu Z, et al.
    The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.
    Plant Cell, 2014. 26(4): p. 1512-1524
    [PMID:24755456]
  3. Ko SS, et al.
    The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice.
    Plant Cell, 2014. 26(6): p. 2486-2504
    [PMID:24894043]