PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016480146.1
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ERF
Protein Properties Length: 324aa    MW: 35481.9 Da    PI: 7.8515
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016480146.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
             AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                     +y+GVr+++ +g+WvAeIr+p++   r+r +lg+f tae+Aa+a+++a+  l+g
                     59****999.**********954...5999*******************98876 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5103222.33960117IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.2E-2960117IPR001471AP2/ERF domain
PfamPF008472.3E-1160105IPR001471AP2/ERF domain
SuperFamilySSF541719.15E-2160118IPR016177DNA-binding domain
CDDcd000188.37E-2160117No hitNo description
SMARTSM003801.1E-3760123IPR001471AP2/ERF domain
PRINTSPR003673.1E-106172IPR001471AP2/ERF domain
PRINTSPR003673.1E-108399IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006970Biological Processresponse to osmotic stress
GO:0009414Biological Processresponse to water deprivation
GO:0009738Biological Processabscisic acid-activated signaling pathway
GO:0009744Biological Processresponse to sucrose
GO:0009749Biological Processresponse to glucose
GO:0010119Biological Processregulation of stomatal movement
GO:0010353Biological Processresponse to trehalose
GO:0010449Biological Processroot meristem growth
GO:0010896Biological Processregulation of triglyceride catabolic process
GO:0031930Biological Processmitochondria-nucleus signaling pathway
GO:0032880Biological Processregulation of protein localization
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0048316Biological Processseed development
GO:0048527Biological Processlateral root development
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 324 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtTranscription regulator that probably binds to the GCC-box pathogenesis-related promoter element. Binds also to the S-box (5'-CACTTCCA-3') photosynthesis-associated nuclear genes-related (PhANGs-related) promoter element, and thus acts as a transcription inhibitor. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. May have a function in the deetiolation process. Confers sensitivity to abscisic acid (ABA), and regulates the ABA signaling pathway during seed germination, upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol. Plays a role in sucrose sensing or signaling, especially at low fluence far red light. Also involved in plant response to glucose treatment, especially at low concentration and in young seedlings. Required for the trehalose-mediated root inhibition and starch accumulation in cotyledons, probably by inhibiting starch breakdown. However, seems to not be involved in sugar-mediated senescence. Required for the ABA-dependent beta-amino-butyric acid (BABA) signaling pathway. BABA primes ABA synthesis and promotes resistance to drought and salt, and leads to a prime callose accumulation that confers resistance against necrotrophic pathogens such as A.brassicicola and P.cucumerina. Seems to be involved in resistance to S.sclerotiorum probably by regulating the ABA-mediated stomatal closure apparently by antagonistic interaction with oxalate. Negative regulator of low water potential-induced Pro accumulation whose effect is decreased by high levels of sugar. {ECO:0000269|PubMed:10629000, ECO:0000269|PubMed:10950871, ECO:0000269|PubMed:10972884, ECO:0000269|PubMed:10972885, ECO:0000269|PubMed:11115891, ECO:0000269|PubMed:11172073, ECO:0000269|PubMed:11439129, ECO:0000269|PubMed:11851911, ECO:0000269|PubMed:11996676, ECO:0000269|PubMed:12136027, ECO:0000269|PubMed:12529517, ECO:0000269|PubMed:12857824, ECO:0000269|PubMed:15053765, ECO:0000269|PubMed:15118859, ECO:0000269|PubMed:15502012, ECO:0000269|PubMed:16098105, ECO:0000269|PubMed:16113213, ECO:0000269|PubMed:16339784, ECO:0000269|PubMed:16844907, ECO:0000269|PubMed:17031512, ECO:0000269|PubMed:9144963, ECO:0000269|PubMed:9418043, ECO:0000269|PubMed:9634591}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00674SELEXTransfer from GRMZM2G093595Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Only in young seedlings by ABA, imbibition, glucose, 2-deoxy-glucose (2DG), trehalose, and osmotic stress. {ECO:0000269|PubMed:12857824, ECO:0000269|PubMed:12970489, ECO:0000269|PubMed:17031512}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_016480146.10.0PREDICTED: ethylene-responsive transcription factor ABI4
SwissprotA0MES86e-38ABI4_ARATH; Ethylene-responsive transcription factor ABI4
TrEMBLA0A1S4AU830.0A0A1S4AU83_TOBAC; ethylene-responsive transcription factor ABI4
STRINGXP_009776130.10.0(Nicotiana sylvestris)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G40220.12e-30ERF family protein
Publications ? help Back to Top
  1. Lin LL, et al.
    Identification of microRNA 395a in 24-epibrassinolide-regulated root growth of Arabidopsis thaliana using microRNA arrays.
    Int J Mol Sci, 2013. 14(7): p. 14270-86
  2. Easlon HM, et al.
    The physiological basis for genetic variation in water use efficiency and carbon isotope composition in Arabidopsis thaliana.
    Photosyn. Res., 2014. 119(1-2): p. 119-29
  3. Duarte GT, et al.
    Involvement of microRNA-related regulatory pathways in the glucose-mediated control of Arabidopsis early seedling development.
    J. Exp. Bot., 2013. 64(14): p. 4301-12
  4. Seifert GJ,Xue H,Acet T
    The Arabidopsis thaliana FASCICLIN LIKE ARABINOGALACTAN PROTEIN 4 gene acts synergistically with abscisic acid signalling to control root growth.
    Ann. Bot., 2014. 114(6): p. 1125-33
  5. Joseph MP, et al.
    The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development.
    Plant Physiol., 2014. 165(3): p. 1203-1220
  6. Cruz TM,Carvalho RF,Richardson DN,Duque P
    Abscisic acid (ABA) regulation of Arabidopsis SR protein gene expression.
    Int J Mol Sci, 2014. 15(10): p. 17541-64
  7. Chen C,Twito S,Miller G
    New cross talk between ROS, ABA and auxin controlling seed maturation and germination unraveled in APX6 deficient Arabidopsis seeds.
    Plant Signal Behav, 2014. 9(12): p. e976489
  8. Kong D, et al.
    Arabidopsis glutamate receptor homolog3.5 modulates cytosolic Ca2+ level to counteract effect of abscisic acid in seed germination.
    Plant Physiol., 2015. 167(4): p. 1630-42
  9. Lu Y, et al.
    ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis.
    J. Exp. Bot., 2015. 66(9): p. 2763-71
  10. Mukhopadhyay P,Tyagi AK
    OsTCP19 influences developmental and abiotic stress signaling by modulating ABI4-mediated pathways.
    Sci Rep, 2015. 5: p. 9998
  11. Yao X,Li J,Liu J,Liu K
    An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.
    J. Exp. Bot., 2015. 66(20): p. 6431-45
  12. Zhang ZW,Zhang GC,Zhu F,Zhang DW,Yuan S
    The roles of tetrapyrroles in plastid retrograde signaling and tolerance to environmental stresses.
    Planta, 2015. 242(6): p. 1263-76
  13. Kang J, et al.
    Abscisic acid transporters cooperate to control seed germination.
    Nat Commun, 2015. 6: p. 8113
  14. Dong Z, et al.
    Abscisic Acid Antagonizes Ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis.
    Mol Plant, 2016. 9(1): p. 126-135
  15. Garcia L, et al.
    The cytochrome c oxidase biogenesis factor AtCOX17 modulates stress responses in Arabidopsis.
    Plant Cell Environ., 2016. 39(3): p. 628-44
  16. Shu K, et al.
    ABSCISIC ACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LOCUS C transcription.
    J. Exp. Bot., 2016. 67(1): p. 195-205
  17. Li L, et al.
    TOR-inhibitor insensitive-1 (TRIN1) regulates cotyledons greening in Arabidopsis.
    Front Plant Sci, 2015. 6: p. 861
  18. Shu K, et al.
    ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein levels.
    Plant J., 2016. 85(3): p. 348-61
  19. Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF
    Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development.
    PLoS Genet., 2016. 12(2): p. e1005833
  20. Li T,Wu XY,Li H,Song JH,Liu JY
    A Dual-Function Transcription Factor, AtYY1, Is a Novel Negative Regulator of the Arabidopsis ABA Response Network.
    Mol Plant, 2016. 9(5): p. 650-661
  21. Lee SA, et al.
    Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue.
    Mol Plant, 2016. 9(6): p. 870-84
  22. Xu X, et al.
    Convergence of light and chloroplast signals for de-etiolation through ABI4-HY5 and COP1.
    Nat Plants, 2016. 2(6): p. 16066
  23. Waszczak C, et al.
    SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions.
    Plant Cell, 2016. 28(8): p. 1844-59
  24. Hsiao YC,Hsu YF,Chen YC,Chang YL,Wang CS
    A WD40 protein, AtGHS40, negatively modulates abscisic acid degrading and signaling genes during seedling growth under high glucose conditions.
    J. Plant Res., 2016. 129(6): p. 1127-1140
  25. Li PC, et al.
    Arabidopsis YL1/BPG2 Is Involved in Seedling Shoot Response to Salt Stress through ABI4.
    Sci Rep, 2016. 6: p. 30163
  26. Eckstein A,Krzeszowiec W,Banaś AK,Janowiak F,Gabryś H
    Abscisic acid and blue light signaling pathways in chloroplast movements in Arabidopsis mesophyll.
    Acta Biochim. Pol., 2016. 63(3): p. 449-58
  27. Wilson ME,Mixdorf M,Berg RH,Haswell ES
    Plastid osmotic stress influences cell differentiation at the plant shoot apex.
    Development, 2016. 143(18): p. 3382-93
  28. Yu FW,Zhu XF,Li GJ,Kronzucker HJ,Shi WM
    The Chloroplast Protease AMOS1/EGY1 Affects Phosphate Homeostasis under Phosphate Stress.
    Plant Physiol., 2016. 172(2): p. 1200-1208
  29. Huang X,Zhang X,Gong Z,Yang S,Shi Y
    ABI4 represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis.
    Plant J., 2017. 89(2): p. 354-365
  30. Gu L, et al.
    An RRM-containing mei2-like MCT1 plays a negative role in the seed germination and seedling growth of Arabidopsis thaliana in the presence of ABA.
    Plant Physiol. Biochem., 2016. 109: p. 273-279
  31. Yan J, et al.
    The miR165/166 Mediated Regulatory Module Plays Critical Roles in ABA Homeostasis and Response in Arabidopsis thaliana.
    PLoS Genet., 2016. 12(11): p. e1006416
  32. Chen YS, et al.
    Two MYB-related transcription factors play opposite roles in sugar signaling in Arabidopsis.
    Plant Mol. Biol., 2017. 93(3): p. 299-311
  33. Mu Y, et al.
    BASIC PENTACYSTEINE Proteins Repress ABSCISIC ACID INSENSITIVE4 Expression via Direct Recruitment of the Polycomb-Repressive Complex 2 in Arabidopsis Root Development.
    Plant Cell Physiol., 2017. 58(3): p. 607-621
  34. Li K,Yang F,Miao Y,Song CP
    Abscisic acid signaling is involved in regulating the mitogen-activated protein kinase cascade module, AIK1-MKK5-MPK6.
    Plant Signal Behav, 2017. 12(5): p. e1321188
  35. Xiao X,Cheng X,Yin K,Li H,Qiu JL
    Abscisic acid negatively regulates post-penetration resistance of Arabidopsis to the biotrophic powdery mildew fungus.
    Sci China Life Sci, 2017. 60(8): p. 891-901
  36. Liu S, et al.
    Transcriptome profiling of genes involved in induced systemic salt tolerance conferred by Bacillus amyloliquefaciens FZB42 in Arabidopsis thaliana.
    Sci Rep, 2017. 7(1): p. 10795
  37. Shu K,Zhou W,Yang W
    APETALA 2-domain-containing transcription factors: focusing on abscisic acid and gibberellins antagonism.
    New Phytol., 2018. 217(3): p. 977-983
  38. Barczak-Brzyżek A, et al.
    Abscisic Acid Insensitive 4 transcription factor is an important player in the response of Arabidopsis thaliana to two-spotted spider mite (Tetranychus urticae) feeding.
    Exp. Appl. Acarol., 2017. 73(3-4): p. 317-326
  39. Min JH, et al.
    Arabidopsis Basic Helix-Loop-Helix 34 (bHLH34) Is Involved in Glucose Signaling through Binding to a GAGA Cis-Element.
    Front Plant Sci, 2017. 8: p. 2100
  40. Cheng Y,Zhang X,Sun T,Tian Q,Zhang WH
    Glutamate Receptor Homolog3.4 is Involved in Regulation of Seed Germination Under Salt Stress in Arabidopsis.
    Plant Cell Physiol., 2018. 59(5): p. 978-988
  41. Kacprzak SM, et al.
    Plastid-to-Nucleus Retrograde Signalling during Chloroplast Biogenesis Does Not Require ABI4.
    Plant Physiol., 2019. 179(1): p. 18-23
  42. Liu Y, et al.
    Trithorax-group protein ATX5 mediates the glucose response via impacting the HY1-ABI4 signaling module.
    Plant Mol. Biol., 2018. 98(6): p. 495-506