PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID MLOC_46407.3
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
Family bHLH
Protein Properties Length: 226aa    MW: 24043.4 Da    PI: 7.3507
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
MLOC_46407.3genomeIBSCView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
           HLH  7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksL 54
                  ++ErrRR+++N+++  Lr+++Pk +      K++ a+iL  A++Y+k+L
                  79*********************66......****************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.8664897IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474597.85E-1751113IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.3E-1454103IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.0E-95497IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000834.47E-1455102No hitNo description
Gene3DG3DSA:, basic helix-loop-helix (bHLH) domain
CDDcd048733.34E-7172213No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 226 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5gnj_A2e-1450107663Transcription factor MYC2
5gnj_B2e-1450107663Transcription factor MYC2
5gnj_E2e-1450107663Transcription factor MYC2
5gnj_F2e-1450107663Transcription factor MYC2
5gnj_G2e-1450107663Transcription factor MYC2
5gnj_I2e-1450107663Transcription factor MYC2
5gnj_M2e-1450107663Transcription factor MYC2
5gnj_N2e-1450107663Transcription factor MYC2
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Functional Description ? help Back to Top
Source Description
UniProtTranscriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation (PubMed:18641265, PubMed:28507175). {ECO:0000269|PubMed:17416732, ECO:0000269|PubMed:18641265, ECO:0000269|PubMed:28507175}.
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By high-salt stress, cold stress and abscisic acid (ABA) treatment.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK3551180.0AK355118.1 Hordeum vulgare subsp. vulgare mRNA for predicted protein, complete cds, clone: NIASHv1016C20.
GenBankDQ1515360.0DQ151536.1 Hordeum vulgare subsp. vulgare inducer of CBF expression 2 (ICE2) mRNA, partial cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_020184163.11e-136transcription factor ICE1-like
SwissprotQ9LSE28e-85ICE1_ARATH; Transcription factor ICE1
TrEMBLA0A287MES71e-158A0A287MES7_HORVV; Uncharacterized protein
STRINGMLOC_46407.11e-156(Hordeum vulgare)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G26744.43e-81bHLH family protein
Publications ? help Back to Top
  1. Chen Y, et al.
    Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398.
    BMC Biol., 2013. 11: p. 121
  2. Xu F, et al.
    Increased drought tolerance through the suppression of ESKMO1 gene and overexpression of CBF-related genes in Arabidopsis.
    PLoS ONE, 2014. 9(9): p. e106509
  3. Jiang W,Wu J,Zhang Y,Yin L,Lu J
    Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses.
    Protoplasma, 2015. 252(5): p. 1361-74
  4. Lang Z,Zhu J
    OST1 phosphorylates ICE1 to enhance plant cold tolerance.
    Sci China Life Sci, 2015. 58(3): p. 317-8
  5. Juan JX, et al.
    Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene.
    Genet. Mol. Res., 2015. 14(1): p. 597-608
  6. Lee HG,Seo PJ
    The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis.
    Plant J., 2015. 82(6): p. 962-77
  7. Horst RJ, et al.
    Molecular Framework of a Regulatory Circuit Initiating Two-Dimensional Spatial Patterning of Stomatal Lineage.
    PLoS Genet., 2015. 11(7): p. e1005374
  8. Lee JH,Jung JH,Park CM
    INDUCER OF CBF EXPRESSION 1 integrates cold signals into FLOWERING LOCUS C-mediated flowering pathways in Arabidopsis.
    Plant J., 2015. 84(1): p. 29-40
  9. Wang CL,Zhang SC,Qi SD,Zheng CC,Wu CA
    Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11.
    Gene, 2016. 575(2 Pt 1): p. 206-12
  10. Lee JH,Park CM
    Integration of photoperiod and cold temperature signals into flowering genetic pathways in Arabidopsis.
    Plant Signal Behav, 2015. 10(11): p. e1089373
  11. Su F, et al.
    Burkholderia phytofirmans PsJN reduces impact of freezing temperatures on photosynthesis in Arabidopsis thaliana.
    Front Plant Sci, 2015. 6: p. 810
  12. Klermund C, et al.
    LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls.
    Plant Cell, 2016. 28(3): p. 646-60
  13. Chen L, et al.
    NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.
    Development, 2016. 143(9): p. 1600-11
  14. Lu X, et al.
    A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana.
    Plant Physiol. Biochem., 2017. 113: p. 78-88
  15. Deng C,Ye H,Fan M,Pu T,Yan J
    The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis.
    Plant Signal Behav, 2017. 12(5): p. e1316442
  16. de Marcos A, et al.
    A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development.
    Plant Physiol., 2017. 174(2): p. 823-842
  17. Kim SH, et al.
    Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis.
    Nucleic Acids Res., 2017. 45(11): p. 6613-6627
  18. Pal S, et al.
    TransDetect Identifies a New Regulatory Module Controlling Phosphate Accumulation.
    Plant Physiol., 2017. 175(2): p. 916-926
  19. Zhao C, et al.
    MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability.
    Dev. Cell, 2017. 43(5): p. 618-629.e5
  20. Li H, et al.
    MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis.
    Dev. Cell, 2017. 43(5): p. 630-642.e4
  21. Lee JH,Jung JH,Park CM
    Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis.
    Plant Cell, 2017. 29(11): p. 2817-2830
  22. Liu Y,Zhou J
    MAPping Kinase Regulation of ICE1 in Freezing Tolerance.
    Trends Plant Sci., 2018. 23(2): p. 91-93
  23. Xie H, et al.
    Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana.
    Plant Cell Physiol., 2019. 60(1): p. 152-165