PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID MLOC_36351.2
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
Family bHLH
Protein Properties Length: 245aa    MW: 26873.4 Da    PI: 7.2664
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
MLOC_36351.2genomeIBSCView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH438e-1470121155
                   CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
           HLH   1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                   r+ +hn+ Er RR+++N+ ++ Lr+llP +   +++Kls   ++ ++ +YI +Lq
  MLOC_36351.2  70 RKISHNAYERDRRKQLNELYSDLRSLLPDS---DHTKLSIPITVSRVLKYIPELQ 121
                   6889**************************...9******************998 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474594.06E-1668137IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088813.54869120IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000832.53E-1069124No hitNo description
PfamPF000105.5E-1170121IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.101.1E-1270136IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003539.1E-975126IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 245 aa     Download sequence    Send to blast
MGHQTQMFDD PFASSMSSLD ADIFSVAGGL HPSQWPGLDH DVSLAPAANN GTSSGGYGSP  60
GGGDGSGSHR KISHNAYERD RRKQLNELYS DLRSLLPDSD HTKLSIPITV SRVLKYIPEL  120
QKQVDGLEKK KEELTRASCK PGVLTMKENT VPIVSATCLD EREIMVQVSL VSTMAGALPM  180
SKCIKVLENE GLRLISSSTS AFQNRTFYSL HLQRTQRTMS KECPAFCEEL ENALTQKAGL  240
RLHHQ
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination (PubMed:16887895, PubMed:17559517, PubMed:21331630, PubMed:26224556). Promotes the accumulation of mugineic acid family phytosiderophores (MAs) (PubMed:17559517). Required for ethylene-mediated signaling during iron deficiency responses (PubMed:21112958). Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain (PubMed:21331630). {ECO:0000269|PubMed:16887895, ECO:0000269|PubMed:17559517, ECO:0000269|PubMed:21112958, ECO:0000269|PubMed:21331630, ECO:0000269|PubMed:26224556}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapMLOC_36351.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Strongly induced in both roots and shoots during iron (Fe) deficiency stress (PubMed:16887895, PubMed:18025467, PubMed:19737364, PubMed:21112958, PubMed:21331630, PubMed:24280375). Ethylene enhances the iron deficiency-mediated induction (PubMed:21112958). Induced by arsenate (AsV25 and AsV50); this induction is repressed by nitric oxide (NO) (PubMed:26793232). Accumulates under cadmium (Cd) stress; this induction is inhibited by the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine (Aza) (PubMed:27412910). {ECO:0000269|PubMed:16887895, ECO:0000269|PubMed:18025467, ECO:0000269|PubMed:19737364, ECO:0000269|PubMed:21112958, ECO:0000269|PubMed:21331630, ECO:0000269|PubMed:24280375, ECO:0000269|PubMed:26793232, ECO:0000269|PubMed:27412910}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2517460.0AK251746.1 Hordeum vulgare subsp. vulgare cDNA clone: FLbaf134m16, mRNA sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_020193397.11e-153transcription factor bHLH101-like
SwissprotQ0JFZ09e-96IRO2_ORYSJ; Protein IRON-RELATED TRANSCRIPTION FACTOR 2
TrEMBLM0VQ630.0M0VQ63_HORVV; Uncharacterized protein
STRINGMLOC_36351.11e-165(Hordeum vulgare)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G56970.17e-30bHLH family protein
Publications ? help Back to Top
  1. Ogo Y, et al.
    The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions.
    Plant J., 2007. 51(3): p. 366-77
    [PMID:17559517]
  2. Kobayashi T, et al.
    The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes.
    Plant J., 2009. 60(6): p. 948-61
    [PMID:19737364]
  3. Wu J, et al.
    Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa.
    J. Exp. Bot., 2011. 62(2): p. 667-74
    [PMID:21112958]
  4. Ogo Y, et al.
    OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.
    Plant Mol. Biol., 2011. 75(6): p. 593-605
    [PMID:21331630]
  5. Yang A,Li Y,Xu Y,Xu Y,Zhang WH
    A receptor-like protein RMC is involved in regulation of iron acquisition in rice.
    J. Exp. Bot., 2013. 64(16): p. 5009-20
    [PMID:24014863]
  6. Itai RN,Ogo Y,Kobayashi T,Nakanishi H,Nishizawa NK
    Rice genes involved in phytosiderophore biosynthesis are synchronously regulated during the early stages of iron deficiency in roots.
    Rice (N Y), 2013. 6(1): p. 16
    [PMID:24280375]
  7. Masuda H,Aung MS,Nishizawa NK
    Iron biofortification of rice using different transgenic approaches.
    Rice (N Y), 2013. 6(1): p. 40
    [PMID:24351075]
  8. Kobayashi T,Nakanishi Itai R,Nishizawa NK
    Iron deficiency responses in rice roots.
    Rice (N Y), 2014. 7(1): p. 27
    [PMID:26224556]
  9. Singh AP, et al.
    Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.).
    Front Plant Sci, 2015. 6: p. 1272
    [PMID:26793232]
  10. Yang A,Zhang WH
    A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice.
    Plant Cell Physiol., 2016. 57(6): p. 1271-80
    [PMID:27257291]
  11. Feng SJ, et al.
    Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium.
    Plant Cell Environ., 2016. 39(12): p. 2629-2649
    [PMID:27412910]