PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID 462877146
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis
Family AP2
Protein Properties Length: 636aa    MW: 70264 Da    PI: 9.9854
Description AP2 family protein
Gene Model
Gene Model ID Type Source Coding Sequence
462877146genomeTefView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP246.87.5e-1570127153
        AP2   1 sgykGVrwdkkrgrWvAeIrd.pseng...kr.krfslgkfgtaeeAakaaiaarkkl 53 
                s+++GV++++++gr++A+++d  s+n    k+ k+++lg ++ +e Aa+a++ a++k+
  462877146  70 SRFRGVSRHRWTGRFEAHLWDkNSWNPtqrKKgKQVYLGAYDEEEAAARAYDLAALKK 127
                789******************888855666447**********************986 PP

2AP250.45.4e-16136187155
        AP2   1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                s+y+GV +++++grW+A+I   +   g +k+ +lg++ t eeAa+a++ a+ +++g
  462877146 136 SKYRGVARHHQNGRWEARIGRvF---G-NKYLYLGTYSTQEEAARAYDIAAIEYKG 187
                89****************99966...3.6***********************9986 PP

3AP249.69.8e-16305364155
        AP2   1 sgykGVrwdkkrgrWvAeIrd.pseng...kr.krfslgkfgtaeeAakaaiaarkkleg 55 
                s+++GV++++++gr++A+++d  s+n    k+ k+++lg ++ +e Aa+a++ a++k++g
  462877146 305 SRFRGVSRHRWTGRFEAHLWDkNSWNPtqrKKgKQVYLGAYDEEEAAARAYDLAALKYWG 364
                789******************888855666447*************************98 PP

4AP250.45.4e-16407458155
        AP2   1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                s+y+GV +++++grW+A+I   +   g +k+ +lg++ t eeAa+a++ a+ +++g
  462877146 407 SKYRGVARHHQNGRWEARIGRvF---G-NKYLYLGTYSTQEEAARAYDIAAIEYKG 458
                89****************99966...3.6***********************9986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF541712.29E-1170128IPR016177DNA-binding domain
PfamPF008475.1E-1170127IPR001471AP2/ERF domain
SMARTSM003801.4E-1071129IPR001471AP2/ERF domain
PROSITE profilePS5103215.97571126IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.106.9E-1171126IPR001471AP2/ERF domain
PfamPF008476.8E-11136187IPR001471AP2/ERF domain
CDDcd000182.31E-22136195No hitNo description
SuperFamilySSF541715.04E-17136196IPR016177DNA-binding domain
SMARTSM003801.7E-26137201IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.8E-17137195IPR001471AP2/ERF domain
PROSITE profilePS5103218.979137195IPR001471AP2/ERF domain
PfamPF008473.1E-12305364IPR001471AP2/ERF domain
SuperFamilySSF541712.22E-16305373IPR016177DNA-binding domain
SMARTSM003801.1E-24306378IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.101.9E-15306373IPR001471AP2/ERF domain
PROSITE profilePS5103219.809306372IPR001471AP2/ERF domain
PfamPF008476.8E-11407458IPR001471AP2/ERF domain
SuperFamilySSF541715.04E-17407467IPR016177DNA-binding domain
CDDcd000182.31E-22407466No hitNo description
SMARTSM003801.7E-26408472IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.8E-17408466IPR001471AP2/ERF domain
PROSITE profilePS5103218.979408466IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006110Biological Processregulation of glycolytic process
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009744Biological Processresponse to sucrose
GO:0019432Biological Processtriglyceride biosynthetic process
GO:1901959Biological Processpositive regulation of cutin biosynthetic process
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 636 aa     Download sequence    Send to blast
MENMVMVKNE IENFSGSAPA VMINASEGGE ASQVVRRRRR EPALLAPISG GANSSGIGKP  60
LPSITVKRSS RFRGVSRHRW TGRFEAHLWD KNSWNPTQRK KGKQVYLGAY DEEEAAARAY  120
DLAALKKSSG FSRGVSKYRG VARHHQNGRW EARIGRVFGN KYLYLGTYST QEEAARAYDI  180
AAIEYKGVNA VTNFDLRSYI TWLKPSTPLQ LLLRSFRAAA ISASEAAFGA GSVAVMENMV  240
MVKNEIENFS GSAPAVMINA SEGGEASQVV RRRRREPALL APISGGANSS GIGKPLPSIT  300
VKRSSRFRGV SRHRWTGRFE AHLWDKNSWN PTQRKKGKQV YLGAYDEEEA AARAYDLAAL  360
KYWGPTTYTN FPVMDYEKEL KIMENLTKEE YLASLRRKSS GFSRGVSKYR GVARHHQNGR  420
WEARIGRVFG NKYLYLGTYS TQEEAARAYD IAAIEYKGVN AVTNFDLRSY ITWLKPSTPV  480
TFNPEALVMQ ATPAEQVLQT ETQMLPRGNP FLLDHNALPG SSSISQEASM ISPGGMRKRG  540
SSTALSLLFK SSMFRQLVEK NSDAEEAAGG VREAGAHPGE AYEYHNFFQA DGPDMCDLFS  600
SGCGNAPNAF HGQIACYDDG ESAATWNGFG NMSSLP
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1330338PTQRKKGKQ
Functional Description ? help Back to Top
Source Description
UniProtMay be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269|PubMed:12084821, ECO:0000269|PubMed:15500472, ECO:0000269|PubMed:15753106, ECO:0000269|PubMed:16553903, ECO:0000269|PubMed:16632590, ECO:0000269|PubMed:9733529}.
Cis-element ? help Back to Top
SourceLink
PlantRegMap462877146
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269|PubMed:15753106}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY6851121e-169AY685112.1 Oryza sativa (japonica cultivar-group) transcription factor AP2D4 (AP2D4) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_004961382.10.0ethylene-responsive transcription factor WRI1
SwissprotQ6X5Y62e-87WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBLA0A1E5WDW30.0A0A1E5WDW3_9POAL; Ethylene-responsive transcription factor WRI1
STRINGSi022186m0.0(Setaria italica)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MonocotsOGMP41623473
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G54320.14e-77AP2 family protein
Publications ? help Back to Top
  1. Kim HU, et al.
    Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues.
    FEBS Open Bio, 2013. 4: p. 25-32
    [PMID:24363987]
  2. Wu XL,Liu ZH,Hu ZH,Huang RZ
    BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed.
    J Integr Plant Biol, 2014. 56(6): p. 582-93
    [PMID:24393360]
  3. Shigematsu H, et al.
    Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana.
    PLoS ONE, 2014. 9(1): p. e87724
    [PMID:24475319]
  4. Kim HU, et al.
    Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth.
    Plant Biotechnol. J., 2015. 13(9): p. 1346-59
    [PMID:25790072]
  5. Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P
    Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues.
    BMC Plant Biol., 2015. 15: p. 192
    [PMID:26253704]
  6. Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M
    Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds.
    Plant Biotechnol. J., 2016. 14(5): p. 1241-50
    [PMID:26503031]
  7. Li Q, et al.
    Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus.
    Front Plant Sci, 2015. 6: p. 1015
    [PMID:26635841]
  8. Hofvander P, et al.
    Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism.
    Plant Biotechnol. J., 2016. 14(9): p. 1883-98
    [PMID:26914183]
  9. Shen SL, et al.
    CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange.
    J. Exp. Bot., 2016. 67(14): p. 4105-15
    [PMID:27194737]
  10. Adhikari ND,Bates PD,Browse J
    WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds.
    Plant Physiol., 2016. 171(1): p. 179-91
    [PMID:27208047]
  11. Kim MJ,Jang IC,Chua NH
    The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor.
    Plant Physiol., 2016. 171(3): p. 1951-64
    [PMID:27246098]
  12. Bhattacharya S,Das N,Maiti MK
    Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea.
    Plant Physiol. Biochem., 2016. 107: p. 204-213
    [PMID:27314514]
  13. Ma W, et al.
    14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1.
    Plant J., 2016. 88(2): p. 228-235
    [PMID:27322486]
  14. Li D, et al.
    MYB89 Transcription Factor Represses Seed Oil Accumulation.
    Plant Physiol., 2017. 173(2): p. 1211-1225
    [PMID:27932421]
  15. Ivarson E, et al.
    Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre.
    Front Plant Sci, 2016. 7: p. 2032
    [PMID:28119714]
  16. An D, et al.
    Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves.
    Front Plant Sci, 2017. 8: p. 34
    [PMID:28174580]
  17. Zhai Z,Liu H,Shanklin J
    Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis.
    Plant Cell, 2017. 29(4): p. 871-889
    [PMID:28314829]
  18. Jin J, et al.
    Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm.
    Sci Rep, 2017. 7(1): p. 439
    [PMID:28348403]
  19. Kong Q, et al.
    The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots.
    J. Exp. Bot., 2017. 68(16): p. 4627-4634
    [PMID:28981783]
  20. Kang NK, et al.
    Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina.
    Biotechnol Biofuels, 2017. 10: p. 231
    [PMID:29046718]
  21. Pellaud S, et al.
    WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis.
    New Phytol., 2018. 217(1): p. 245-260
    [PMID:29105089]
  22. Hanano A,Almousally I,Shaban M,Murphy DJ
    Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1.
    J. Exp. Bot., 2018. 69(7): p. 1781-1794
    [PMID:29394403]