PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Cucsa.342870.2
Common NameCsa_3G866510, LOC101216825
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis
Family ARF
Protein Properties Length: 967aa    MW: 106651 Da    PI: 5.0526
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Cucsa.342870.2genomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B376.82.4e-24149250199
                     EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSS CS
              B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrs 88 
                     f+k+lt sd++++g +++p++ ae+        + ++++ ++l+++d++ ++W++++iyr++++r++lt+GW+ Fv a++L++gD+v+F   +++
  Cucsa.342870.2 149 FCKTLTASDTSTHGGFSVPRRAAEKLfppldytM-QPPT-QELIVRDLHDNTWTFRHIYRGQPKRHLLTTGWSLFVGAKRLRAGDSVLFI--RDE 239
                     99*********************999*****954.4444.38************************************************..457 PP

                     EE..EEEEE-S CS
              B3  89 efelvvkvfrk 99 
                     + +l+++v+r+
  Cucsa.342870.2 240 KSQLLIGVRRA 250
                     7778****997 PP

2Auxin_resp113.52e-37275358183
      Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                     aahaa+++s+F+++YnPra++seFv++++k++k ++ +++s+GmRf m+fete+s +rr++Gt+vg+sdldp+rWp+SkWr+L+
  Cucsa.342870.2 275 AAHAAANRSPFTIFYNPRACPSEFVIPLAKYRKCVYgTQLSAGMRFGMMFETEESGKRRYMGTIVGISDLDPLRWPGSKWRNLQ 358
                     79*********************************99*********************************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019361.05E-46136278IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.104.4E-42142263IPR015300DNA-binding pseudobarrel domain
CDDcd100171.35E-22148249No hitNo description
SMARTSM010191.7E-24149251IPR003340B3 DNA binding domain
PfamPF023621.1E-21149250IPR003340B3 DNA binding domain
PROSITE profilePS5086313.084149251IPR003340B3 DNA binding domain
PfamPF065071.9E-32275358IPR010525Auxin response factor
PROSITE profilePS5174521.836852936IPR000270PB1 domain
PfamPF023098.6E-9863939IPR033389AUX/IAA domain
SuperFamilySSF542779.81E-9867931No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009734Biological Processauxin-activated signaling pathway
GO:0009908Biological Processflower development
GO:0009942Biological Processlongitudinal axis specification
GO:0010305Biological Processleaf vascular tissue pattern formation
GO:0048364Biological Processroot development
GO:0048507Biological Processmeristem development
GO:0005634Cellular Componentnucleus
GO:0016020Cellular Componentmembrane
GO:0042802Molecular Functionidentical protein binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 967 aa     Download sequence    Send to blast
MGSVEEKLKT SGGLINNAPQ TNLLDEMKLL KEMQDQSGAR KAINSELWHA CAGPLVSLPH  60
VGSLVYYFPQ GHSEQVAVST KRTATSQIPN YPNLPSQLMC QVQNVTLHAD KDSDEIYAQM  120
SLQPVNSEKD VFLVPDFGLR PSKHPNEFFC KTLTASDTST HGGFSVPRRA AEKLFPPLDY  180
TMQPPTQELI VRDLHDNTWT FRHIYRGQPK RHLLTTGWSL FVGAKRLRAG DSVLFIRDEK  240
SQLLIGVRRA NRQQTTLPSS VLSADSMHIG VLAAAAHAAA NRSPFTIFYN PRACPSEFVI  300
PLAKYRKCVY GTQLSAGMRF GMMFETEESG KRRYMGTIVG ISDLDPLRWP GSKWRNLQVE  360
WDEPGCCDKQ NRVSSWEIET PESLFIFPSL TSGLKRPLHG GFLGETDWGS LVKRPMLRVP  420
ENIRGDLSYA PTLCSEPLMK MLLRPQMVNL NGTTLQQDST NNLVKIQDMK DMQNPKMQQL  480
IPTETASPGN QNQHHPGPAQ SDPINPNSSP KANVPGKVQT SVAIESEAPT AADGDKAKYD  540
RDLSASTNQS NPLPPVGGCA EEKLTSNEMN MQTLVNQLSF VNQNQIPMQL QSVSWPMQPQ  600
LESLIQHPQP IDMPQPEYTN SNGLISSLDG DGCLINPSCL PLPGVMRSPG NLSMLGLQDS  660
STVFPEVLNF PLPSTGQDMW DPLNNIRFSS QTNHLISFSH ADASNLNCMA NANIMRDVSD  720
ESNNQSGIYS CSNLEMSNGG STLVDHAVSS TILDDYCTLK DADFPHPSDC LAGNFSSSQD  780
VQSQITSASL GDSQAFSRQE FHDNSAGTSS CNVDFDEGSL LQNGSWKQVV PPLRTYTKVG  840
TCDFYFCYFL ELKDTEVQKA GSVGRSIDVT SFKNYDELCS AIECMFGLEG LLNDPRGSGW  900
KLVYVDYEND VLLIGDDPWE EFVSCVRCIR ILSPSEVQQM SEEGMKLLNS AMMQGINCPM  960
SEGGRS*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A0.013835392Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:14973283, ECO:0000269|PubMed:17553903}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00153DAPTransfer from AT1G19850Download
Motif logo
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankKU3234190.0KU323419.1 UNVERIFIED: Trichosanthes dioica clone TdARF_27 auxin response factor 5-like mRNA, partial sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_011652441.10.0PREDICTED: auxin response factor 5 isoform X2
SwissprotP930240.0ARFE_ARATH; Auxin response factor 5
TrEMBLA0A0A0LE690.0A0A0A0LE69_CUCSA; Auxin response factor
STRINGXP_004154860.10.0(Cucumis sativus)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT1G19850.10.0ARF family protein
Publications ? help Back to Top
  1. Ren Y, et al.
    An integrated genetic and cytogenetic map of the cucumber genome.
    PLoS ONE, 2009. 4(6): p. e5795
    [PMID:19495411]
  2. Guo S, et al.
    Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
    BMC Genomics, 2010. 11: p. 384
    [PMID:20565788]
  3. Li Z, et al.
    RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
    BMC Genomics, 2011. 12: p. 540
    [PMID:22047402]
  4. Chen MK,Wilson RL,Palme K,Ditengou FA,Shpak ED
    ERECTA family genes regulate auxin transport in the shoot apical meristem and forming leaf primordia.
    Plant Physiol., 2013. 162(4): p. 1978-91
    [PMID:23821653]
  5. Huang JB, et al.
    ROP3 GTPase contributes to polar auxin transport and auxin responses and is important for embryogenesis and seedling growth in Arabidopsis.
    Plant Cell, 2014. 26(9): p. 3501-18
    [PMID:25217509]
  6. Qi J, et al.
    Auxin depletion from leaf primordia contributes to organ patterning.
    Proc. Natl. Acad. Sci. U.S.A., 2014. 111(52): p. 18769-74
    [PMID:25512543]
  7. Crawford BC, et al.
    Plant development. Genetic control of distal stem cell fate within root and embryonic meristems.
    Science, 2015. 347(6222): p. 655-9
    [PMID:25612610]
  8. Zhang Y, et al.
    Regulation of oncogene expression in T-DNA-transformed host plant cells.
    PLoS Pathog., 2015. 11(1): p. e1004620
    [PMID:25615824]
  9. Robert HS, et al.
    Plant embryogenesis requires AUX/LAX-mediated auxin influx.
    Development, 2015. 142(4): p. 702-11
    [PMID:25617434]
  10. Krogan NT,Berleth T
    The identification and characterization of specific ARF-Aux/IAA regulatory modules in plant growth and development.
    Plant Signal Behav, 2015. 10(4): p. e992748
    [PMID:25830553]
  11. Ckurshumova W,Berleth T
    Overcoming recalcitrance - Auxin response factor functions in plant regeneration.
    Plant Signal Behav, 2015. 10(7): p. e993293
    [PMID:26098229]
  12. Wendrich JR, et al.
    A set of domain-specific markers in the Arabidopsis embryo.
    Plant Reprod, 2015. 28(3-4): p. 153-60
    [PMID:26216537]
  13. Wu MF, et al.
    Auxin-regulated chromatin switch directs acquisition of flower primordium founder fate.
    Elife, 2015. 4: p. e09269
    [PMID:26460543]
  14. Yamaguchi N,Jeong CW,Nole-Wilson S,Krizek BA,Wagner D
    AINTEGUMENTA and AINTEGUMENTA-LIKE6/PLETHORA3 Induce LEAFY Expression in Response to Auxin to Promote the Onset of Flower Formation in Arabidopsis.
    Plant Physiol., 2016. 170(1): p. 283-93
    [PMID:26537561]
  15. Müller CJ, et al.
    PHABULOSA Mediates an Auxin Signaling Loop to Regulate Vascular Patterning in Arabidopsis.
    Plant Physiol., 2016. 170(2): p. 956-70
    [PMID:26637548]
  16. Herud O,Weijers D,Lau S,Jürgens G
    Auxin responsiveness of the MONOPTEROS-BODENLOS module in primary root initiation critically depends on the nuclear import kinetics of the Aux/IAA inhibitor BODENLOS.
    Plant J., 2016. 85(2): p. 269-77
    [PMID:26714008]
  17. Luo S, et al.
    Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis.
    Front Plant Sci, 2015. 6: p. 1156
    [PMID:26734051]
  18. Yamaguchi N,Wu MF,Winter CM,Wagner D
    LEAFY and Polar Auxin Transport Coordinately Regulate Arabidopsis Flower Development.
    Plants (Basel), 2014. 3(2): p. 251-65
    [PMID:27135503]
  19. Krogan NT,Marcos D,Weiner AI,Berleth T
    The auxin response factor MONOPTEROS controls meristem function and organogenesis in both the shoot and root through the direct regulation of PIN genes.
    New Phytol., 2016. 212(1): p. 42-50
    [PMID:27441727]
  20. Fendrych M,Leung J,Friml J
    TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls.
    Elife, 2018.
    [PMID:27627746]
  21. Matthes M,Torres-Ruiz RA
    Boronic acid treatment phenocopies monopteros by affecting PIN1 membrane stability and polar auxin transport in Arabidopsis thaliana embryos.
    Development, 2016. 143(21): p. 4053-4062
    [PMID:27697905]
  22. Bhatia N, et al.
    Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis.
    Curr. Biol., 2016. 26(23): p. 3202-3208
    [PMID:27818174]
  23. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  24. Möller BK, et al.
    Auxin response cell-autonomously controls ground tissue initiation in the early Arabidopsis embryo.
    Proc. Natl. Acad. Sci. U.S.A., 2017. 114(12): p. E2533-E2539
    [PMID:28265057]
  25. Carey NS,Krogan NT
    The role of AUXIN RESPONSE FACTORs in the development and differential growth of inflorescence stems.
    Plant Signal Behav, 2017. 12(4): p. e1307492
    [PMID:28340328]
  26. Guan C, et al.
    Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis.
    Curr. Biol., 2017. 27(19): p. 2940-2950.e4
    [PMID:28943086]
  27. Gaillochet C, et al.
    Control of plant cell fate transitions by transcriptional and hormonal signals.
    Elife, 2018.
    [PMID:29058667]
  28. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  29. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]