PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_009126884.1
Common NameWRKY74
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family WRKY
Protein Properties Length: 322aa    MW: 35295 Da    PI: 10.4526
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_009126884.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY96.81.4e-30246303359
                     -SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
            WRKY   3 DgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                     D+++WrKYGqK++kgs++pr+YY+C++  gCp++k+ver+ +dp ++++tYegeH+h+
  XP_009126884.1 246 DEFSWRKYGQKPIKGSPHPRGYYKCSTFrGCPARKHVERALDDPAMLIVTYEGEHHHK 303
                     99***********************8766****************************8 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF105333.3E-16194242IPR018872Zn-cluster domain
Gene3DG3DSA:2.20.25.801.8E-32232304IPR003657WRKY domain
PROSITE profilePS5081129.826239305IPR003657WRKY domain
SuperFamilySSF1182902.62E-26242304IPR003657WRKY domain
SMARTSM007747.1E-37244304IPR003657WRKY domain
PfamPF031061.4E-26246303IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009737Biological Processresponse to abscisic acid
GO:0009938Biological Processnegative regulation of gibberellic acid mediated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0042742Biological Processdefense response to bacterium
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005516Molecular Functioncalmodulin binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:1990841Molecular Functionpromoter-specific chromatin binding
Sequence ? help Back to Top
Protein Sequence    Length: 322 aa     Download sequence    Send to blast
MAVDLMRFPK MDDQKAIQEA ASQGLQSMEH LIRVLSTNRP EQQSNVDCSE ITDFTVSKFK  60
TVISLLNRTG HARFRRGPVH STSSSPPIQQ SQIVKTAQPE APVVSQPARA TTSLPPSRPS  120
VTLDFTKPSI FGSNSKSSEL EFSKENFSVS LSSSFMTSAL TGDGSVSKGS SIFAPSQTVT  180
SSGKPPLAGG QPYRKRCIEH EHSQNFSGKI SGSGHGKCHC KKRKNRPKRT VRVPAISSKI  240
ADIPPDEFSW RKYGQKPIKG SPHPRGYYKC STFRGCPARK HVERALDDPA MLIVTYEGEH  300
HHKQSPMQMN VSGVNDLVFA SA
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A2e-20232306275WRKY transcription factor 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Bra.57180.0leaf| root
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: In young, mature and senescent leaves. {ECO:0000269|PubMed:11722756}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269|PubMed:26433201}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00464DAPTransfer from AT4G31550Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapXP_009126884.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269|PubMed:11722756, ECO:0000269|PubMed:26433201}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankKF4300790.0KF430079.1 Brassica rapa WRKY transcription factor 74 (WRKY74) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001288982.10.0probable WRKY transcription factor 11
SwissprotQ9SV150.0WRK11_ARATH; Probable WRKY transcription factor 11
TrEMBLM4D4780.0M4D478_BRARP; Uncharacterized protein
TrEMBLV5REP40.0V5REP4_BRACM; WRKY transcription factor 74
STRINGBra011282.1-P0.0(Brassica rapa)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT4G31550.21e-173WRKY DNA-binding protein 11
Publications ? help Back to Top
  1. Haas BJ, et al.
    Full-length messenger RNA sequences greatly improve genome annotation.
    Genome Biol., 2002. 3(6): p. RESEARCH0029
    [PMID:12093376]
  2. Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D
    DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro.
    Plant Methods, 2010. 6: p. 25
    [PMID:21108821]
  3. Brand LH, et al.
    Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA.
    PLoS ONE, 2013. 8(10): p. e75177
    [PMID:24146751]
  4. Tang J, et al.
    Characterization and co-expression analysis of WRKY orthologs involved in responses to multiple abiotic stresses in Pak-choi (Brassica campestris ssp. chinensis).
    BMC Plant Biol., 2013. 13: p. 188
    [PMID:24267479]
  5. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  6. Ali MA,Wieczorek K,Kreil DP,Bohlmann H
    The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots.
    PLoS ONE, 2014. 9(7): p. e102360
    [PMID:25033038]
  7. Jiang CH, et al.
    Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.
    J. Exp. Bot., 2016. 67(1): p. 157-74
    [PMID:26433201]
  8. Ali MA, et al.
    Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis.
    J. Plant Physiol., 2018. 226: p. 12-21
    [PMID:29689430]