PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_009103999.1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family WRKY
Protein Properties Length: 283aa    MW: 31972.7 Da    PI: 6.1448
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_009103999.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY85.84e-27126186259
                     --SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS
            WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                     +D ++WrKYGqK++ +++fprsY+rCt++   gC+++k+v++ +++p+++ itY g+H+++
  XP_009103999.1 126 EDTFSWRKYGQKQILNTKFPRSYFRCTHKytqGCKATKQVQKLESEPRMFSITYIGNHTCN 186
                     8***************************99999**************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.802.5E-26112186IPR003657WRKY domain
SuperFamilySSF1182901.01E-24119187IPR003657WRKY domain
PROSITE profilePS5081120.885120188IPR003657WRKY domain
SMARTSM007742.5E-38125187IPR003657WRKY domain
PfamPF031061.0E-24126185IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009862Biological Processsystemic acquired resistance, salicylic acid mediated signaling pathway
GO:0009864Biological Processinduced systemic resistance, jasmonic acid mediated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0050832Biological Processdefense response to fungus
GO:1900056Biological Processnegative regulation of leaf senescence
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 283 aa     Download sequence    Send to blast
MDIASNNKAI KLKVRDQLLQ GHEMTTKVQQ LLSQDGSDLG PAKDLVEKIL GSFNDIISAL  60
DSFEPISPSA LVTAVEGSQN ASCDNDGKLE DSGDSHKKLG PVKGKRGCYK RKKKSETWTV  120
ESTVLEDTFS WRKYGQKQIL NTKFPRSYFR CTHKYTQGCK ATKQVQKLES EPRMFSITYI  180
GNHTCNTNEV TPKIKPCIHH DEIITDSEEI QSPSLMTSMK EEEENHHHGS STESDLQLVW  240
QEMLVFEEEH HHHHEAVYGC GETSTSINGL DSADLWSWQQ FSV
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5w3x_B1e-191271831874Disease resistance protein RRS1
5w3x_D1e-191271831874Disease resistance protein RRS1
6ir8_A8e-20125185868OsWRKY45
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Bra.31000.0bud| flower| leaf
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: In flowers, first observed in both stigmatic papillae and the flower abscission zone, later confined to the abscission zone (PubMed:17310369). In leaves, level increases gradually up to the point of leaf senescence (PubMed:17310369, PubMed:22268143). {ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:22268143}.
UniprotTISSUE SPECIFICITY: Expressed in leaves and flowers. {ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:22268143}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways (PubMed:14742872, PubMed:16623907, PubMed:17310369, PubMed:28576847). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression (PubMed:24104863). Plays an important but not indispensable role in jasmonate and salicylic acid signaling (PubMed:18713432). Regulates positively the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways (PubMed:14742872, PubMed:16623907, PubMed:18713432, PubMed:28837631). Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways (PubMed:28837631, PubMed:16623907, PubMed:22325892). Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2) (PubMed:22325892, PubMed:16623907). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS) (PubMed:16623907). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression (PubMed:17310369). Negative regulator of SA biosynthesis (PubMed:28837631). Negative regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1) (PubMed:17313163). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae) (PubMed:25339349). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:17310369, PubMed:22268143). Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000 (PubMed:26433201). {ECO:0000250|UniProtKB:Q9SUP6, ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:16623907, ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17313163, ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:24104863, ECO:0000269|PubMed:25339349, ECO:0000269|PubMed:26433201, ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631, ECO:0000303|PubMed:28837631}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00408DAPTransfer from AT3G56400Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapXP_009103999.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Regulated by MYB44 (PubMed:23067202, PubMed:23603962). Basal expression levels require the presence of endogeneous salicylic acid (SA) (PubMed:14742872, PubMed:17310369). Induced by reactive oxygen species (ROS) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Induced by SA; early induction is NPR1-independent, but full-scale induction is NPR1-dependent (PubMed:14742872, PubMed:22325892, PubMed:22268143, PubMed:26433201). Up-regulated by benzothiadiazole (BTH) (PubMed:26433201). Repressed by jasmonic acid (MeJA) by both COI1-dependent and COI1-independent pathways (PubMed:14742872, PubMed:18713432). Triggered by the pathogenic compatible bacteria E.carotovora subsp. carotovora SCC3193 (PubMed:14742872). Induced by P.syringae pv. tomato DC3000 (PubMed:17965588, PubMed:22325892). Stimulated by ATX1 (PubMed:17965588). Up-regulated by E.amylovora (PubMed:22316300). Accumulates during leaf and flower senescence (PubMed:17310369). Induced expression upon simultaneous feeding by caterpillars (e.g. P.xylostella) and aphids (e.g. B.brassicae) at a low density, but lower levels in plants induced with both caterpillars and a high aphid density (PubMed:25339349). Responsive to rhizobacterium B.cereus AR156 in leaves (PubMed:26433201). {ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17965588, ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:23067202, ECO:0000269|PubMed:23603962, ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:25339349, ECO:0000269|PubMed:26433201}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankKF4300760.0KF430076.1 Brassica rapa WRKY transcription factor 70 (WRKY70) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001288847.10.0probable WRKY transcription factor 70
RefseqXP_013648025.10.0probable WRKY transcription factor 70
SwissprotQ9LY001e-137WRK70_ARATH; Probable WRKY transcription factor 70
TrEMBLM4CGA60.0M4CGA6_BRARP; Uncharacterized protein
STRINGBra003239.1-P0.0(Brassica rapa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM40612757
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G56400.11e-123WRKY DNA-binding protein 70
Publications ? help Back to Top
  1. Zhang X, et al.
    Arabidopsis cysteine-rich receptor-like kinase 45 positively regulates disease resistance to Pseudomonas syringae.
    Plant Physiol. Biochem., 2013. 73: p. 383-91
    [PMID:24215930]
  2. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  3. Brosché M, et al.
    Transcriptomics and functional genomics of ROS-induced cell death regulation by RADICAL-INDUCED CELL DEATH1.
    PLoS Genet., 2014. 10(2): p. e1004112
    [PMID:24550736]
  4. Bao F, et al.
    Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses.
    New Phytol., 2014. 202(4): p. 1320-34
    [PMID:24611624]
  5. Wang F, et al.
    TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.
    Front Plant Sci, 2015. 6: p. 108
    [PMID:25774162]
  6. Jiang CH, et al.
    Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.
    J. Exp. Bot., 2016. 67(1): p. 157-74
    [PMID:26433201]
  7. Kaurilind E,Xu E,Brosché M
    A genetic framework for H2O2 induced cell death in Arabidopsis thaliana.
    BMC Genomics, 2015. 16: p. 837
    [PMID:26493993]
  8. Rodriguez-Salus M, et al.
    The Synthetic Elicitor 2-(5-Bromo-2-Hydroxy-Phenyl)-Thiazolidine-4-Carboxylic Acid Links Plant Immunity to Hormesis.
    Plant Physiol., 2016. 170(1): p. 444-58
    [PMID:26530314]
  9. Onkokesung N,Reichelt M,van Doorn A,Schuurink RC,Dicke M
    Differential Costs of Two Distinct Resistance Mechanisms Induced by Different Herbivore Species in Arabidopsis.
    Plant Physiol., 2016. 170(2): p. 891-906
    [PMID:26603653]
  10. Chen J, et al.
    Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses.
    Plant Cell, 2017. 29(6): p. 1425-1439
    [PMID:28576847]
  11. Li J,Zhong R,Palva ET
    WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis.
    PLoS ONE, 2017. 12(8): p. e0183731
    [PMID:28837631]
  12. Zhou M, et al.
    WRKY70 prevents axenic activation of plant immunity by direct repression of SARD1.
    New Phytol., 2018. 217(2): p. 700-712
    [PMID:29076543]