PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G67060.1
Common NameBHLH88, EN118, HEC1, K21H1.2
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 241aa    MW: 26229.8 Da    PI: 10.077
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G67060.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH26.21.4e-08137177954
                  HHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
          HLH   9 ErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                   r RR+ri +++  L++l+P       +K++ a++L +A++Y+k L
  AT5G67060.1 137 ARHRRERISERIRILQRLVPGG-----TKMDTASMLDEAIHYVKFL 177
                  699*****************87.....8****************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.434128177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.103.7E-14129187IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474598.51E-15132188IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003538.3E-14134183IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000106.8E-6138177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000835.31E-6151182No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010500Biological Processtransmitting tissue development
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0048462Biological Processcarpel formation
GO:2000012Biological Processregulation of auxin polar transport
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000030anatomyseptum
PO:0009010anatomyseed
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009052anatomyflower pedicel
PO:0009073anatomystigma
PO:0020003anatomyplant ovule
PO:0020102anatomyportion of transmitting tissue
PO:0025022anatomycollective leaf structure
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0007606developmental stagegynoecium development stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007615developmental stageflower development stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 241 aa     Download sequence    Send to blast
MDSDIMNMMM HQMEKLPEFC NPNSSFFSPD HNNTYPFLFN STHYQSDHSM TNEPGFRYGS  60
GLLTNPSSIS PNTAYSSVFL DKRNNSNNNN NGTNMAAMRE MIFRIAVMQP IHIDPEAVKP  120
PKRRNVRISK DPQSVAARHR RERISERIRI LQRLVPGGTK MDTASMLDEA IHYVKFLKKQ  180
VQSLEEQAVV TGGGGGGGGR VLIGGGGMTA ASGGGGGGGV VMKGCGTVGT HQMVGNAQIL  240
R
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1197205GGRVLIGGG
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible247023_at0.0
Expression AtlasAT5G67060-
AtGenExpressAT5G67060-
ATTED-IIAT5G67060-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in the developing septum, transmitting tract and stigma. {ECO:0000269|PubMed:17855426}.
UniprotTISSUE SPECIFICITY: Flowers, especially in gynoecium. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:17855426}.
Functional Description ? help Back to Top
Source Description
UniProtRequired for the female reproductive tract development and fertility. {ECO:0000269|PubMed:17855426}.
Function -- GeneRIF ? help Back to Top
  1. HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3)
    [PMID: 24576426]
  2. HEC1 acts as a local modulator of auxin and cytokinin responses to control gynoecium development in Arabidopsis.
    [PMID: 26293302]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G67060.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Negatively regulated by ARF3/ETT in the abaxial gynoecium. {ECO:0000269|PubMed:17855426}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9FHA7
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Impaired pollen tube growth. {ECO:0000269|PubMed:17855426}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G67060
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0207420.0AB020742.1 Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K21H1.
GenBankAF4886180.0AF488618.1 Arabidopsis thaliana clone bHLH088 putative bHLH transcription factor mRNA, complete cds.
GenBankAK2289270.0AK228927.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor, complete cds, clone: RAFL16-24-D23.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_201507.11e-180basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ9FHA70.0HEC1_ARATH; Transcription factor HEC1
TrEMBLA0A178UR101e-178A0A178UR10_ARATH; HEC1
STRINGAT5G67060.11e-179(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM15592791
Representative plantOGRP7611568
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Abe H, et al.
    Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell, 2003. 15(1): p. 63-78
    [PMID:12509522]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Brodersen P, et al.
    Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4.
    Plant J., 2006. 47(4): p. 532-46
    [PMID:16813576]
  7. Gremski K,Ditta G,Yanofsky MF
    The HECATE genes regulate female reproductive tract development in Arabidopsis thaliana.
    Development, 2007. 134(20): p. 3593-601
    [PMID:17855426]
  8. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  9. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  10. Vercruyssen L, et al.
    ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development.
    Plant Cell, 2014. 26(1): p. 210-29
    [PMID:24443518]
  11. Sparks EE,Benfey PN
    HEC of a job regulating stem cells.
    Dev. Cell, 2014. 28(4): p. 349-50
    [PMID:24576420]
  12. Schuster C, et al.
    A regulatory framework for shoot stem cell control integrating metabolic, transcriptional, and phytohormone signals.
    Dev. Cell, 2014. 28(4): p. 438-49
    [PMID:24576426]
  13. Schuster C,Gaillochet C,Lohmann JU
    Arabidopsis HECATE genes function in phytohormone control during gynoecium development.
    Development, 2015. 142(19): p. 3343-50
    [PMID:26293302]
  14. Zhu L, et al.
    A Negative Feedback Loop between PHYTOCHROME INTERACTING FACTORs and HECATE Proteins Fine-Tunes Photomorphogenesis in Arabidopsis.
    Plant Cell, 2016. 28(4): p. 855-74
    [PMID:27073231]
  15. Lozano-Sotomayor P, et al.
    Altered expression of the bZIP transcription factor DRINK ME affects growth and reproductive development in Arabidopsis thaliana.
    Plant J., 2016. 88(3): p. 437-451
    [PMID:27402171]
  16. Boex-Fontvieille E, et al.
    An Ethylene-Protected Achilles' Heel of Etiolated Seedlings for Arthropod Deterrence.
    Front Plant Sci, 2016. 7: p. 1246
    [PMID:27625656]
  17. Gaillochet C, et al.
    Control of plant cell fate transitions by transcriptional and hormonal signals.
    Elife, 2018.
    [PMID:29058667]