PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G67060.1
Common NameBHLH88, EN118, HEC1, K21H1.2
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 241aa    MW: 26229.8 Da    PI: 10.077
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G67060.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          HLH   9 ErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                   r RR+ri +++  L++l+P       +K++ a++L +A++Y+k L
                  699*****************87.....8****************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.434128177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474598.51E-15132188IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003538.3E-14134183IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000106.8E-6138177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000835.31E-6151182No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010500Biological Processtransmitting tissue development
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0048462Biological Processcarpel formation
GO:2000012Biological Processregulation of auxin polar transport
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009052anatomyflower pedicel
PO:0020003anatomyplant ovule
PO:0020102anatomyportion of transmitting tissue
PO:0025022anatomycollective leaf structure
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0007606developmental stagegynoecium development stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007615developmental stageflower development stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 241 aa     Download sequence    Send to blast
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT5G67060-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in the developing septum, transmitting tract and stigma. {ECO:0000269|PubMed:17855426}.
UniprotTISSUE SPECIFICITY: Flowers, especially in gynoecium. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:17855426}.
Functional Description ? help Back to Top
Source Description
UniProtRequired for the female reproductive tract development and fertility. {ECO:0000269|PubMed:17855426}.
Function -- GeneRIF ? help Back to Top
  1. HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3)
    [PMID: 24576426]
  2. HEC1 acts as a local modulator of auxin and cytokinin responses to control gynoecium development in Arabidopsis.
    [PMID: 26293302]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Negatively regulated by ARF3/ETT in the abaxial gynoecium. {ECO:0000269|PubMed:17855426}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9FHA7
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Impaired pollen tube growth. {ECO:0000269|PubMed:17855426}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G67060
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0207420.0AB020742.1 Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K21H1.
GenBankAF4886180.0AF488618.1 Arabidopsis thaliana clone bHLH088 putative bHLH transcription factor mRNA, complete cds.
GenBankAK2289270.0AK228927.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor, complete cds, clone: RAFL16-24-D23.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_201507.11e-180basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ9FHA70.0HEC1_ARATH; Transcription factor HEC1
TrEMBLA0A178UR101e-178A0A178UR10_ARATH; HEC1
STRINGAT5G67060.11e-179(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP7611568
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Abe H, et al.
    Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell, 2003. 15(1): p. 63-78
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
  6. Brodersen P, et al.
    Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4.
    Plant J., 2006. 47(4): p. 532-46
  7. Gremski K,Ditta G,Yanofsky MF
    The HECATE genes regulate female reproductive tract development in Arabidopsis thaliana.
    Development, 2007. 134(20): p. 3593-601
  8. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
  9. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  10. Vercruyssen L, et al.
    ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development.
    Plant Cell, 2014. 26(1): p. 210-29
  11. Sparks EE,Benfey PN
    HEC of a job regulating stem cells.
    Dev. Cell, 2014. 28(4): p. 349-50
  12. Schuster C, et al.
    A regulatory framework for shoot stem cell control integrating metabolic, transcriptional, and phytohormone signals.
    Dev. Cell, 2014. 28(4): p. 438-49
  13. Schuster C,Gaillochet C,Lohmann JU
    Arabidopsis HECATE genes function in phytohormone control during gynoecium development.
    Development, 2015. 142(19): p. 3343-50
  14. Zhu L, et al.
    A Negative Feedback Loop between PHYTOCHROME INTERACTING FACTORs and HECATE Proteins Fine-Tunes Photomorphogenesis in Arabidopsis.
    Plant Cell, 2016. 28(4): p. 855-74
  15. Lozano-Sotomayor P, et al.
    Altered expression of the bZIP transcription factor DRINK ME affects growth and reproductive development in Arabidopsis thaliana.
    Plant J., 2016. 88(3): p. 437-451
  16. Boex-Fontvieille E, et al.
    An Ethylene-Protected Achilles' Heel of Etiolated Seedlings for Arthropod Deterrence.
    Front Plant Sci, 2016. 7: p. 1246
  17. Gaillochet C, et al.
    Control of plant cell fate transitions by transcriptional and hormonal signals.
    Elife, 2018.