PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G65320.1
Common NameBHLH99, EN18, MNA5.5
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 296aa    MW: 33761.2 Da    PI: 9.4846
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G65320.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH44.72.3e-14100151155
                  CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  +r++h ++Er+RR+++N+ ++ L++++P +   +s+ ++ a+i e ++ Y+k+L+
  AT5G65320.1 100 QRMNHIAVERNRRKQMNHFLSILKSMMPLS---YSQPNDQASIIEGTISYLKKLE 151
                  79****************************...*******************996 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000834.48E-1297155No hitNo description
Gene3DG3DSA:4.10.280.101.0E-1298155IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474593.66E-1798170IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088815.15499150IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000105.6E-12100151IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003531.2E-8105156IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
PO:0020100anatomyhypocotyl
PO:0001054developmental stagevascular leaf senescent stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
Sequence ? help Back to Top
Protein Sequence    Length: 296 aa     Download sequence    Send to blast
MMFQQDYPHG FSLVETSLSY EMLDYFQNIV VSNSEDVASQ QNSISSSSYS SATLSCSITE  60
QKSHLTEKLS PLRERYGCGD FLSRKRRRRS EKTIVDKENQ RMNHIAVERN RRKQMNHFLS  120
ILKSMMPLSY SQPNDQASII EGTISYLKKL EQRLQSLEAQ LKATKLNQSP NIFSDFFMFP  180
QYSTATATAT ATASSSSSSH HHHKRLEVVA DVEVTMVERH ANIKVLTKTQ PRLLFKIINE  240
FNSLGLSTLH LNLTTSKDMS LFTFSVKVEA DCQLTPSGNE VANTVHEVVR RVHKER
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
18387RKRRR
28389RKRRRRS
3100111RMNHIAVERNRR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.283950.0root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306981160.0
Genevisible247179_at0.0
Expression AtlasAT5G65320-
AtGenExpressAT5G65320-
ATTED-IIAT5G65320-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively in roots, stems, and flowers. {ECO:0000269|PubMed:12679534}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G65320.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by heat treatment. {ECO:0000269|PubMed:12679534}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G65320
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0700950.0AY070095.1 Arabidopsis thaliana unknown protein (At5g65320) mRNA, complete cds.
GenBankAY0967040.0AY096704.1 Arabidopsis thaliana unknown protein (At5g65320) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_201335.10.0basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ9FKQ60.0BH099_ARATH; Transcription factor bHLH99
TrEMBLA0A1P8BDH60.0A0A1P8BDH6_ARATH; Basic helix-loop-helix (BHLH) DNA-binding superfamily protein
STRINGAT5G65320.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM149591619
Representative plantOGRP25315131
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  3. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Hilson P, et al.
    Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications.
    Genome Res., 2004. 14(10B): p. 2176-89
    [PMID:15489341]
  7. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]