PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G63470.2
Common NameMLE2.10, NFYC4, NF-YC4
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family NF-YC
Protein Properties Length: 250aa    MW: 27032.2 Da    PI: 4.7457
Description nuclear factor Y, subunit C4
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G63470.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1NF-YC178.56.2e-56561601102
        NF-YC   1 qlksfwekq...iekatdfknhelPlarikkilkadedvkmisaeaPvllskacelfileltlrswlhaeenkrrtlkksdiaaavtrtdifdflvdi 95 
                  ql++fw++q   ie+++dfknh+lPlarikki+kadedv+misaeaP+l++kacelfilelt+rswlhaeenkrrtl+k+diaaa+trtdifdflvdi
  AT5G63470.2  56 QLQMFWTYQrqeIEQVNDFKNHQLPLARIKKIMKADEDVRMISAEAPILFAKACELFILELTIRSWLHAEENKRRTLQKNDIAAAITRTDIFDFLVDI 153
                  89*****998899************************************************************************************* PP

        NF-YC  96 vprdelk 102
                  vpr+e+k
  AT5G63470.2 154 VPREEIK 160
                  ***9975 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF471131.36E-3523150IPR009072Histone-fold
PfamPF008081.8E-2078141IPR003958Transcription factor CBF/NF-Y/archaeal histone domain
Gene3DG3DSA:1.10.20.107.9E-3779156IPR009072Histone-fold
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009738Biological Processabscisic acid-activated signaling pathway
GO:0010468Biological Processregulation of gene expression
GO:0048586Biological Processregulation of long-day photoperiodism, flowering
GO:0051247Biological Processpositive regulation of protein metabolic process
GO:2000905Biological Processnegative regulation of starch metabolic process
GO:0005634Cellular Componentnucleus
GO:0005829Cellular Componentcytosol
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046982Molecular Functionprotein heterodimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
Sequence ? help Back to Top
Protein Sequence    Length: 250 aa     Download sequence    Send to blast
MDNNNNNNNQ QPPPTSVYPP GSAVTTVIPP PPSGSASIVT GGGATYHHLL QQQQQQLQMF  60
WTYQRQEIEQ VNDFKNHQLP LARIKKIMKA DEDVRMISAE APILFAKACE LFILELTIRS  120
WLHAEENKRR TLQKNDIAAA ITRTDIFDFL VDIVPREEIK EEEDAASALG GGGMVAPAAS  180
GVPYYYPPMG QPAVPGGMMI GRPAMDPSGV YAQPPSQAWQ SVWQNSAGGG DDVSYGSGGS  240
SGHGNLDSQG
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5g49_B6e-5564156395NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT C-3
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1128134RRTLQKN
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.289970.0floral meristem| flower| root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1865327020.0
Genevisible247388_s_at1e-84
Expression AtlasAT5G63470-
AtGenExpressAT5G63470-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Ubiquitous. Present in etiolated seedlings. {ECO:0000269|PubMed:11250072, ECO:0000269|PubMed:17322342}.
Functional Description ? help Back to Top
Source Description
UniProtStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters (By similarity). Involved in the abscisic acid (ABA) signaling pathway. {ECO:0000250, ECO:0000269|PubMed:17322342}.
Function -- GeneRIF ? help Back to Top
  1. QQS protein binds to the transcriptional regulator AtNF-YC4 (Arabidopsis nuclear factor Y, subunit C4).
    [PMID: 26554020]
  2. expression with QQS reduces susceptibility to pathogens and pests
    [PMID: 29878511]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G63470.2
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G09030, AT1G17590, AT1G21970, AT1G30500, AT1G72830
IntActSearch Q9FMV5
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Altered response to abscisic acid (ABA). {ECO:0000269|PubMed:17322342}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G63470
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0076490.0AB007649.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MLE2.
GenBankAK3170380.0AK317038.1 Arabidopsis thaliana AT5G63470 mRNA, complete cds, clone: RAFL19-94-G17.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001032130.10.0nuclear factor Y, subunit C4
RefseqNP_201152.10.0nuclear factor Y, subunit C4
SwissprotQ9FMV50.0NFYC4_ARATH; Nuclear transcription factor Y subunit C-4
TrEMBLA0A384KX140.0A0A384KX14_ARATH; NF-YC4
TrEMBLB9DG640.0B9DG64_ARATH; AT5G63470 protein
STRINGAT5G63470.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Taira T, et al.
    Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y.
    J. Biol. Chem., 1999. 274(34): p. 24270-9
    [PMID:10446203]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Gusmaroli G,Tonelli C,Mantovani R
    Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana.
    Gene, 2001. 264(2): p. 173-85
    [PMID:11250072]
  4. Gusmaroli G,Tonelli C,Mantovani R
    Regulation of novel members of the Arabidopsis thaliana CCAAT-binding nuclear factor Y subunits.
    Gene, 2002. 283(1-2): p. 41-8
    [PMID:11867211]
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  6. Wenkel S, et al.
    CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis.
    Plant Cell, 2006. 18(11): p. 2971-84
    [PMID:17138697]
  7. Warpeha KM, et al.
    The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis.
    Plant Physiol., 2007. 143(4): p. 1590-600
    [PMID:17322342]
  8. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  9. Kumimoto RW,Zhang Y,Siefers N,Holt BF
    NF-YC3, NF-YC4 and NF-YC9 are required for CONSTANS-mediated, photoperiod-dependent flowering in Arabidopsis thaliana.
    Plant J., 2010. 63(3): p. 379-91
    [PMID:20487380]
  10. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
    [PMID:22065421]
  11. Hackenberg D, et al.
    Studies on differential nuclear translocation mechanism and assembly of the three subunits of the Arabidopsis thaliana transcription factor NF-Y.
    Mol Plant, 2012. 5(4): p. 876-88
    [PMID:22199235]
  12. Calvenzani V, et al.
    Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
    PLoS ONE, 2012. 7(8): p. e42902
    [PMID:22912760]
  13. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  14. Li L, et al.
    QQS orphan gene regulates carbon and nitrogen partitioning across species via NF-YC interactions.
    Proc. Natl. Acad. Sci. U.S.A., 2015. 112(47): p. 14734-9
    [PMID:26554020]
  15. Hwang YH, et al.
    Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time.
    Plant Cell Rep., 2016. 35(4): p. 857-65
    [PMID:26754793]
  16. Liu X, et al.
    The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.
    Nat Commun, 2016. 7: p. 12768
    [PMID:27624486]
  17. Myers ZA, et al.
    NUCLEAR FACTOR Y, Subunit C (NF-YC) Transcription Factors Are Positive Regulators of Photomorphogenesis in Arabidopsis thaliana.
    PLoS Genet., 2016. 12(9): p. e1006333
    [PMID:27685091]
  18. Tang Y, et al.
    Arabidopsis NF-YCs Mediate the Light-Controlled Hypocotyl Elongation via Modulating Histone Acetylation.
    Mol Plant, 2017. 10(2): p. 260-273
    [PMID:27876642]
  19. Zhao H, et al.
    The Arabidopsis thaliana Nuclear Factor Y Transcription Factors.
    Front Plant Sci, 2016. 7: p. 2045
    [PMID:28119722]
  20. Qi M, et al.
    QQS orphan gene and its interactor NF-YC4 reduce susceptibility to pathogens and pests.
    Plant Biotechnol. J., 2019. 17(1): p. 252-263
    [PMID:29878511]