PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G47230.1
Common NameATERF5, ATERF-5, AtMACD1, ERF102, ERF5, ERF-5, MQL5_9
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 300aa    MW: 33809.9 Da    PI: 4.7948
Description ethylene responsive element binding factor 5
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G47230.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP266.93.8e-21155205255
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  +y+GVr+++ +g+++AeIrdp+++g   r++lg+f+ta eAa+a+++a+ +l+g
  AT5G47230.1 155 HYRGVRQRP-WGKFAAEIRDPNKRG--SRVWLGTFDTAIEAARAYDEAAFRLRG 205
                  8********.**********96655..*************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000187.55E-33154213No hitNo description
Gene3DG3DSA:3.30.730.102.5E-33154214IPR001471AP2/ERF domain
SuperFamilySSF541718.5E-23155215IPR016177DNA-binding domain
SMARTSM003801.8E-37155219IPR001471AP2/ERF domain
PROSITE profilePS5103224.434155213IPR001471AP2/ERF domain
PfamPF008474.3E-15155205IPR001471AP2/ERF domain
PRINTSPR003671.1E-10156167IPR001471AP2/ERF domain
PRINTSPR003671.1E-10179195IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006952Biological Processdefense response
GO:0009409Biological Processresponse to cold
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 300 aa     Download sequence    Send to blast
MATPNEVSAL WFIEKHLLDE ASPVATDPWM KHESSSATES SSDSSSIIFG SSSSSFAPID  60
FSESVCKPEI IDLDTPRSME FLSIPFEFDS EVSVSDFDFK PSNQNQNQFE PELKSQIRKP  120
PLKISLPAKT EWIQFAAENT KPEVTKPVSE EEKKHYRGVR QRPWGKFAAE IRDPNKRGSR  180
VWLGTFDTAI EAARAYDEAA FRLRGSKAIL NFPLEVGKWK PRADEGEKKR KRDDDEKVTV  240
VEKVLKTEQS VDVNGGETFP FVTSNLTELC DWDLTGFLNF PLLSPLSPHP PFGYSQLTVV
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A4e-31154216163ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.95120.0root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306951370.0
Genevisible248799_at0.0
Expression AtlasAT5G47230-
AtGenExpressAT5G47230-
ATTED-IIAT5G47230-
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-3 of ERF/AP2 transcription factor family (ATERF-5). The protein contains one AP2 domain. There are 18 members in this subfamily including ATERF-1, ATERF-2, AND ATERF-5.
UniProtActs as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:9756931}.
Function -- GeneRIF ? help Back to Top
  1. ERF5 may play an important role in plant innate immunity, likely through coordinating chitin and other defense pathways in plants in response to different pathogens.
    [PMID: 21936663]
  2. Reduced inducibilty in ERF5 constitutive overexepressors was consistent with suppression of SA-mediated signalling, as was an increased susceptibility to avirulent Pseudomonas syringae.
    [PMID: 22563431]
  3. ERF5 and ERF6 form a missing link between the previously observed stress-induced 1-aminocyclopropane-1-carboxylic acid accumulation and DELLA-mediated cell cycle exit and execute a dual role by regulating both stress tolerance and growth inhibition.
    [PMID: 23553636]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00549DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G47230.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2). Wounding as well as cold stress induction does not require EIN2. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by jasmonate, Alternaria brassicicola, exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress. {ECO:0000269|PubMed:10715325}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT3G20770 (A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDethylene
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G47230
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0081070.0AB008107.1 Arabidopsis thaliana AtERF-5 mRNA for ethylene responsive element binding factor 5, complete cds.
GenBankAB0181170.0AB018117.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MQL5.
GenBankAF3857090.0AF385709.1 Arabidopsis thaliana AT5g47230/MQL5_9 mRNA, complete cds.
GenBankAK1175680.0AK117568.1 Arabidopsis thaliana At5g47230 mRNA for putative ethylene responsive element binding factor 5 (ATERF5), complete cds, clone: RAFL17-21-N19.
GenBankAY0780140.0AY078014.1 Arabidopsis thaliana AT5g47230/MQL5_9 mRNA, complete cds.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_568679.10.0ethylene responsive element binding factor 5
SwissprotO803410.0EF102_ARATH; Ethylene-responsive transcription factor 5
TrEMBLA0A178UKK90.0A0A178UKK9_ARATH; ERF5
STRINGAT5G47230.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM10281650
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Fujimoto SY,Ohta M,Usui A,Shinshi H,Ohme-Takagi M
    Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression.
    Plant Cell, 2000. 12(3): p. 393-404
    [PMID:10715325]
  2. Furutani I, et al.
    The SPIRAL genes are required for directional control of cell elongation in Aarabidopsis thaliana.
    Development, 2000. 127(20): p. 4443-53
    [PMID:11003843]
  3. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  4. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
    [PMID:11910074]
  5. Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM
    A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol., 2003. 132(2): p. 1020-32
    [PMID:12805630]
  6. M
    Brassinosteroids promote root growth in Arabidopsis.
    Plant Physiol., 2003. 133(3): p. 1261-71
    [PMID:14526105]
  7. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  8. Catala R, et al.
    Mutations in the Ca2+/H+ transporter CAX1 increase CBF/DREB1 expression and the cold-acclimation response in Arabidopsis.
    Plant Cell, 2003. 15(12): p. 2940-51
    [PMID:14630965]
  9. Navarro L, et al.
    The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis.
    Plant Physiol., 2004. 135(2): p. 1113-28
    [PMID:15181213]
  10. Guan Y,Nothnagel EA
    Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures.
    Plant Physiol., 2004. 135(3): p. 1346-66
    [PMID:15235117]
  11. Scheible WR, et al.
    Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen.
    Plant Physiol., 2004. 136(1): p. 2483-99
    [PMID:15375205]
  12. Miao Y,Laun T,Zimmermann P,Zentgraf U
    Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis.
    Plant Mol. Biol., 2004. 55(6): p. 853-67
    [PMID:15604721]
  13. Danon A,Miersch O,Felix G,Camp RG,Apel K
    Concurrent activation of cell death-regulating signaling pathways by singlet oxygen in Arabidopsis thaliana.
    Plant J., 2005. 41(1): p. 68-80
    [PMID:15610350]
  14. Stanley Kim H, et al.
    Transcriptional divergence of the duplicated oxidative stress-responsive genes in the Arabidopsis genome.
    Plant J., 2005. 41(2): p. 212-20
    [PMID:15634198]
  15. Devoto A, et al.
    Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
    Plant Mol. Biol., 2005. 58(4): p. 497-513
    [PMID:16021335]
  16. Lee BH,Henderson DA,Zhu JK
    The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell, 2005. 17(11): p. 3155-75
    [PMID:16214899]
  17. Suzuki N, et al.
    Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c.
    Plant Physiol., 2005. 139(3): p. 1313-22
    [PMID:16244138]
  18. Vergnolle C, et al.
    The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions.
    Plant Physiol., 2005. 139(3): p. 1217-33
    [PMID:16258011]
  19. Taki N, et al.
    12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis.
    Plant Physiol., 2005. 139(3): p. 1268-83
    [PMID:16258017]
  20. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  21. Babula D, et al.
    Genes involved in biosynthesis and signalisation of ethylene in Brassica oleracea and Arabidopsis thaliana: identification and genome comparative mapping of specific gene homologues.
    Theor. Appl. Genet., 2006. 112(3): p. 410-20
    [PMID:16311726]
  22. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  23. Town CD, et al.
    Comparative genomics of Brassica oleracea and Arabidopsis thaliana reveal gene loss, fragmentation, and dispersal after polyploidy.
    Plant Cell, 2006. 18(6): p. 1348-59
    [PMID:16632643]
  24. AbuQamar S, et al.
    Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection.
    Plant J., 2006. 48(1): p. 28-44
    [PMID:16925600]
  25. Jung J, et al.
    The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis.
    Planta, 2007. 225(3): p. 575-88
    [PMID:16937017]
  26. Zhu Y, et al.
    Arabidopsis NRP1 and NRP2 encode histone chaperones and are required for maintaining postembryonic root growth.
    Plant Cell, 2006. 18(11): p. 2879-92
    [PMID:17122067]
  27. Qutob D, et al.
    Phytotoxicity and innate immune responses induced by Nep1-like proteins.
    Plant Cell, 2006. 18(12): p. 3721-44
    [PMID:17194768]
  28. Xin Z,Mandaokar A,Chen J,Last RL,Browse J
    Arabidopsis ESK1 encodes a novel regulator of freezing tolerance.
    Plant J., 2007. 49(5): p. 786-99
    [PMID:17316173]
  29. Ma S,Bohnert HJ
    Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression.
    Genome Biol., 2007. 8(4): p. R49
    [PMID:17408486]
  30. Hectors K,Prinsen E,De Coen W,Jansen MA,Guisez Y
    Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation show specific changes in morphology and gene expression in the absence of stress symptoms.
    New Phytol., 2007. 175(2): p. 255-70
    [PMID:17587374]
  31. Dombrecht B, et al.
    MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis.
    Plant Cell, 2007. 19(7): p. 2225-45
    [PMID:17616737]
  32. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  33. Chawade A,Br
    Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors.
    BMC Genomics, 2007. 8: p. 304
    [PMID:17764576]
  34. Walley JW, et al.
    Mechanical stress induces biotic and abiotic stress responses via a novel cis-element.
    PLoS Genet., 2007. 3(10): p. 1800-12
    [PMID:17953483]
  35. Yoo SD,Cho YH,Tena G,Xiong Y,Sheen J
    Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling.
    Nature, 2008. 451(7180): p. 789-95
    [PMID:18273012]
  36. Giraud E, et al.
    The absence of ALTERNATIVE OXIDASE1a in Arabidopsis results in acute sensitivity to combined light and drought stress.
    Plant Physiol., 2008. 147(2): p. 595-610
    [PMID:18424626]
  37. Ribot C,Zimmerli C,Farmer EE,Reymond P,Poirier Y
    Induction of the Arabidopsis PHO1;H10 gene by 12-oxo-phytodienoic acid but not jasmonic acid via a CORONATINE INSENSITIVE1-dependent pathway.
    Plant Physiol., 2008. 147(2): p. 696-706
    [PMID:18434606]
  38. Veyres N, et al.
    The Arabidopsis sweetie mutant is affected in carbohydrate metabolism and defective in the control of growth, development and senescence.
    Plant J., 2008. 55(4): p. 665-86
    [PMID:18452589]
  39. Xu J, et al.
    Activation of MAPK kinase 9 induces ethylene and camalexin biosynthesis and enhances sensitivity to salt stress in Arabidopsis.
    J. Biol. Chem., 2008. 283(40): p. 26996-7006
    [PMID:18693252]
  40. Gong W, et al.
    The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.
    Mol Plant, 2008. 1(1): p. 27-41
    [PMID:19802365]
  41. Son GH, et al.
    Ethylene-responsive element-binding factor 5, ERF5, is involved in chitin-induced innate immunity response.
    Mol. Plant Microbe Interact., 2012. 25(1): p. 48-60
    [PMID:21936663]
  42. Wathugala DL, et al.
    The Mediator subunit SFR6/MED16 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways.
    New Phytol., 2012. 195(1): p. 217-30
    [PMID:22494141]
  43. Moffat CS, et al.
    ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis.
    PLoS ONE, 2012. 7(4): p. e35995
    [PMID:22563431]
  44. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  45. Dubois M, et al.
    Ethylene Response Factor6 acts as a central regulator of leaf growth under water-limiting conditions in Arabidopsis.
    Plant Physiol., 2013. 162(1): p. 319-32
    [PMID:23553636]
  46. Mase K, et al.
    Ethylene-responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death.
    Mol. Plant Microbe Interact., 2013. 26(8): p. 868-79
    [PMID:23617414]
  47. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  48. Riechmann JL,Meyerowitz EM
    The AP2/EREBP family of plant transcription factors.
    Biol. Chem., 1998. 379(6): p. 633-46
    [PMID:9687012]
  49. Hao D,Ohme-Takagi M,Sarai A
    Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant.
    J. Biol. Chem., 1998. 273(41): p. 26857-61
    [PMID:9756931]