PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G47220.1
Common NameATERF2, ATERF-2, ERF101, ERF2, ERF-2, MQL5.7
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 243aa    MW: 26797 Da    PI: 5.4801
Description ethylene responsive element binding factor 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G47220.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP262.41e-19116166255
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  +y+GVr+++ +g+++AeIrdp +ng  +r++lg+f tae+Aa a++ a+ +++g
  AT5G47220.1 116 HYRGVRQRP-WGKFAAEIRDPAKNG--ARVWLGTFETAEDAALAYDIAAFRMRG 166
                  8********.***********9987..*************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000181.50E-32115174No hitNo description
Gene3DG3DSA:3.30.730.102.8E-32115174IPR001471AP2/ERF domain
PfamPF008472.2E-13116166IPR001471AP2/ERF domain
PROSITE profilePS5103224.54116174IPR001471AP2/ERF domain
SMARTSM003805.3E-41116180IPR001471AP2/ERF domain
SuperFamilySSF541712.81E-23116176IPR016177DNA-binding domain
PRINTSPR003674.5E-11117128IPR001471AP2/ERF domain
PRINTSPR003674.5E-11140156IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0001944Biological Processvasculature development
GO:0009864Biological Processinduced systemic resistance, jasmonic acid mediated signaling pathway
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0051301Biological Processcell division
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 243 aa     Download sequence    Send to blast
MYGQCNIESD YALLESITRH LLGGGGENEL RLNESTPSSC FTESWGGLPL KENDSEDMLV  60
YGLLKDAFHF DTSSSDLSCL FDFPAVKVEP TENFTAMEEK PKKAIPVTET AVKAKHYRGV  120
RQRPWGKFAA EIRDPAKNGA RVWLGTFETA EDAALAYDIA AFRMRGSRAL LNFPLRVNSG  180
EPDPVRITSK RSSSSSSSSS SSTSSSENGK LKRRRKAENL TSEVVQVKCE VGDETRVDEL  240
LVS
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2gcc_A5e-43112181170ATERF1
3gcc_A5e-43112181170ATERF1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.197310.0floral meristem| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible248794_at0.0
Expression AtlasAT5G47220-
AtGenExpressAT5G47220-
ATTED-IIAT5G47220-
Functional Description ? help Back to Top
Source Description
TAIREncodes a member of the ERF (ethylene response factor) subfamily B-3 of ERF/AP2 transcription factor family (ATERF-2). The protein contains one AP2 domain. Functions as activator of GCC boxdependent transcription. Positive regulator of JA-responsive defense genes and resistance to F. oxysporum and enhances JA inhibition of root elongation.
UniProtActs as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Involved in disease resistance pathways. {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630, ECO:0000269|PubMed:9756931}.
Function -- GeneRIF ? help Back to Top
  1. Expression of several ethylene biosynthetic genes and ethylene-responsive factors, including ERF1 and ERF2, was induced during reoxygenation.
    [PMID: 24506560]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00548DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G47220.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by jasmonate (JA) and Alternaria brassicicola (locally and systemically). Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2) while wounding induction does not require EIN2. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress. {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G32640 (R), AT3G20770 (A), AT5G15850 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G06160(A), AT1G72260(A), AT3G04720(A), AT3G55730(A), AT5G44420(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDethylene
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G47220
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0081040.0AB008104.1 Arabidopsis thaliana AtERF-2 mRNA for ethylene responsive element binding factor 2, complete cds.
GenBankAB0181170.0AB018117.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MQL5.
GenBankAK1751510.0AK175151.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL21-57-E18.
GenBankAK1752290.0AK175229.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2, complete cds, clone: RAFL21-65-J18.
GenBankAK1765320.0AK176532.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-05-B19.
GenBankAK1766060.0AK176606.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-13-E16.
GenBankAK1768250.0AK176825.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-38-F08.
GenBankBT0244700.0BT024470.1 Arabidopsis thaliana At5g47220 mRNA, complete cds.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_199533.11e-180ethylene responsive element binding factor 2
SwissprotO803380.0EF101_ARATH; Ethylene-responsive transcription factor 2
TrEMBLA0A178UCP31e-179A0A178UCP3_ARATH; ERF2
STRINGAT5G47220.11e-179(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM10281650
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Fujimoto SY,Ohta M,Usui A,Shinshi H,Ohme-Takagi M
    Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression.
    Plant Cell, 2000. 12(3): p. 393-404
    [PMID:10715325]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. O
    Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection.
    Plant Physiol., 2002. 128(4): p. 1313-22
    [PMID:11950980]
  4. Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM
    A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol., 2003. 132(2): p. 1020-32
    [PMID:12805630]
  5. McGrath KC, et al.
    Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression.
    Plant Physiol., 2005. 139(2): p. 949-59
    [PMID:16183832]
  6. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  7. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  8. Dombrecht B, et al.
    MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis.
    Plant Cell, 2007. 19(7): p. 2225-45
    [PMID:17616737]
  9. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  10. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  11. Gong W, et al.
    The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.
    Mol Plant, 2008. 1(1): p. 27-41
    [PMID:19802365]
  12. Kouno T,Ezaki B
    Multiple regulation of Arabidopsis AtGST11 gene expression by four transcription factors under abiotic stresses.
    Physiol Plant, 2013. 148(1): p. 97-104
    [PMID:23009097]
  13. Etchells JP,Provost CM,Turner SR
    Plant vascular cell division is maintained by an interaction between PXY and ethylene signalling.
    PLoS Genet., 2012. 8(11): p. e1002997
    [PMID:23166504]
  14. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  15. Tsai KJ,Chou SJ,Shih MC
    Ethylene plays an essential role in the recovery of Arabidopsis during post-anaerobiosis reoxygenation.
    Plant Cell Environ., 2014. 37(10): p. 2391-405
    [PMID:24506560]
  16. Lumba S, et al.
    A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.
    Dev. Cell, 2014. 29(3): p. 360-72
    [PMID:24823379]
  17. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  18. Riechmann JL,Meyerowitz EM
    The AP2/EREBP family of plant transcription factors.
    Biol. Chem., 1998. 379(6): p. 633-46
    [PMID:9687012]
  19. Hao D,Ohme-Takagi M,Sarai A
    Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant.
    J. Biol. Chem., 1998. 273(41): p. 26857-61
    [PMID:9756931]