PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G37020.2
Common NameARF8, ATARF8, FWF, K15O15.5
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ARF
Protein Properties Length: 773aa    MW: 85944.4 Da    PI: 6.3834
Description auxin response factor 8
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G37020.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B371.51.1e-22126226198
                  EEEE-..-HHHHTT-EE--HHH.HTT......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSSEE.. CS
           B3   1 ffkvltpsdvlksgrlvlpkkfaeeh......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrsefel 92 
                  f+k+lt sd++++g +++p++ ae+        ++++  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   ++++ +l
  AT5G37020.2 126 FCKTLTASDTSTHGGFSVPRRAAEKVfppldyTLQPPA-QELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVIFI--RNEKNQL 220
                  99*****************************9666655.49************************************************..5678888 PP

                  EEEEE- CS
           B3  93 vvkvfr 98 
                  +++++ 
  AT5G37020.2 221 FLGIRH 226
                  998876 PP

2Auxin_resp110.81.4e-36252335183
   Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                  aaha +t+s F+v+++Pras+seFv++++k+ ka+  +++svGmRf+m fete+ss rr++Gt++g+sdld+vrWpnS+Wrs+k
  AT5G37020.2 252 AAHASATNSCFTVFFHPRASQSEFVIQLSKYIKAVFhTRISVGMRFRMLFETEESSVRRYMGTITGISDLDSVRWPNSHWRSVK 335
                  799******************************986599******************************************985 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019367.19E-48112255IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.101.8E-39119241IPR015300DNA-binding pseudobarrel domain
CDDcd100173.31E-21125221No hitNo description
PfamPF023621.7E-20126226IPR003340B3 DNA binding domain
SMARTSM010192.8E-22126228IPR003340B3 DNA binding domain
PROSITE profilePS5086312.012126228IPR003340B3 DNA binding domain
PfamPF065075.8E-32252335IPR010525Auxin response factor
SuperFamilySSF542778.5E-6698773No hitNo description
PROSITE profilePS5174522.663705773IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009733Biological Processresponse to auxin
GO:0009734Biological Processauxin-activated signaling pathway
GO:0009908Biological Processflower development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000018anatomyovule primordium
PO:0000037anatomyshoot apex
PO:0000052anatomypetiole vascular system
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020001anatomyovary placenta
PO:0020003anatomyplant ovule
PO:0020006anatomyfunicle
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020102anatomyportion of transmitting tissue
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025074anatomyembryo sac
PO:0025166anatomypapilla cell
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 773 aa     Download sequence    Send to blast
MKLSTSGLGQ QGHEGEKCLN SELWHACAGP LVSLPSSGSR VVYFPQGHSE QVAATTNKEV  60
DGHIPNYPSL PPQLICQLHN VTMHADVETD EVYAQMTLQP LTPEEQKETF VPIELGIPSK  120
QPSNYFCKTL TASDTSTHGG FSVPRRAAEK VFPPLDYTLQ PPAQELIARD LHDVEWKFRH  180
IFRGQPKRHL LTTGWSVFVS AKRLVAGDSV IFIRNEKNQL FLGIRHATRP QTIVPSSVLS  240
SDSMHIGLLA AAAHASATNS CFTVFFHPRA SQSEFVIQLS KYIKAVFHTR ISVGMRFRML  300
FETEESSVRR YMGTITGISD LDSVRWPNSH WRSVKVGWDE STAGERQPRV SLWEIEPLTT  360
FPMYPSLFPL RLKRPWHAGT SSLPDGRGDL GSGLTWLRGG GGEQQGLLPL NYPSVGLFPW  420
MQQRLDLSQM GTDNNQQYQA MLAAGLQNIG GGDPLRQQFV QLQEPHHQYL QQSASHNSDL  480
MLQQQQQQQA SRHLMHAQTQ IMSENLPQQN MRQEVSNQPA GQQQQLQQPD QNAYLNAFKM  540
QNGHLQQWQQ QSEMPSPSFM KSDFTDSSNK FATTASPASG DGNLLNFSIT GQSVLPEQLT  600
TEGWSPKASN TFSEPLSLPQ AYPGKSLALE PGNPQNPSLF GVDPDSGLFL PSTVPRFASS  660
SGDAEASPMS LTDSGFQNSL YSCMQDTTHE LLHGAGQINS SNQTKNFVKV YKSGSVGRSL  720
DISRFSSYHE LREELGKMFA IEGLLEDPLR SGWQLVFVDK ENDILLLGDD PWE
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-154835640388Auxin response factor 5
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.234460.0bud| flower| root| seed| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible249651_at0.0
Expression AtlasAT5G37020-
AtGenExpressAT5G37020-
ATTED-IIAT5G37020-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in sepals at stages 11, 12 and 13 of flower development. Highly expressed in petals at stages 9-10, decreases at stage 11 and disappears after flower stage 12. In anthers, expressed at stage 11 in the tapetum, disappears early in stage 12 when the tapetum degrades and reappears throughout the anther late in stage 12 to persist at least until stage 13. In stamen filaments, expressed at stages 12 to 13, especially near the apical end of the filament. Expressed throughout the gynoecium at early stages up to stage 12, especially strongly in ovules. Expression in gynoecium decreases late in stage 12, but persists through stage 13, especially near the apical end including the style. Expressed in the mesocarp of the fruit and the carpel septum during fruit growth. {ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
UniprotTISSUE SPECIFICITY: Expressed in the whole plant. {ECO:0000269|PubMed:10476078}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a member of the auxin response factor family. Mediates auxin response via expression of auxin regulated genes. Acts redundantly with ARF6 to control stamen elongation and flower maturation. Expression of ARF8 is controlled by miR167.
UniProtAuxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Function -- GeneRIF ? help Back to Top
  1. We characterized the function of auxin response factor 8 in Arabidopsis by investigating a T-DNA insertion line (arf8-1) and overexpression lines (ARF8 OX) of ARF8.
    [PMID: 15469491]
  2. Auxin response factors ARF6 and ARF8 promote jasmonic acid production, while their gene dosage affects the timing of flower maturation.
    [PMID: 16107481]
  3. Enhance expression of microRNA, miR167, which has a complementary sequence to a portion of the AUXIN RESPONSE FACTOR 8 (ARF8), can cause plant fertility defect.
    [PMID: 16699541]
  4. ARF8 restricts signal transduction processes in ovules and growth in pistils until the fruit initiation cue.
    [PMID: 16829592]
  5. Data show that Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction.
    [PMID: 17021043]
  6. The results of this study suggest that similar mechanisms involving auxin signaling exist to inhibit parthenocarpic fruit set in tomato and Arabidopsis.
    [PMID: 17766399]
  7. Phenotypic plasticity of adventitious rooting is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and microRNA abundance. ARF6 and ARF8, targets of miR167, are positive regulators.
    [PMID: 19820192]
  8. We concluded that ARF6 and ARF8, in parallel with AS1 and AS2, repress the class 1 KNOX genes in developing floral organs to allow progression of the development of these organs.
    [PMID: 20007966]
  9. ARF8 influences cell expansion and proliferation during petal growth.
    [PMID: 21421811]
  10. Analyses pinpointed misregulation of ARF8 as a major cause for the developmental aberrations exhibited by VSR transgenic plants, but also for the phenotypes induced during normal viral infection caused by the HcPro-encoding Turnip mosaic virus.
    [PMID: 21589905]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00033PBM25215497Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G37020.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G28130(A), AT2G44810(A), AT5G54510(A)
Regulation -- MicroRNA ? help Back to Top
Source Description
miRTarBaseRegulated by ath-miR167b, ath-miR167a
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDauxin
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G59640
IntActSearch Q9FGV1
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Seedless (parthenocarpic) fruit. {ECO:0000269|PubMed:16829592}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G37020
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF0421960.0AF042196.1 Arabidopsis thaliana auxin response factor 8 (ARF8) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001078672.10.0auxin response factor 8
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLR0GF160.0R0GF16_9BRAS; Auxin response factor
STRINGAT5G37020.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Ulmasov T,Hagen G,Guilfoyle TJ
    Activation and repression of transcription by auxin-response factors.
    Proc. Natl. Acad. Sci. U.S.A., 1999. 96(10): p. 5844-9
    [PMID:10318972]
  2. Ulmasov T,Hagen G,Guilfoyle TJ
    Dimerization and DNA binding of auxin response factors.
    Plant J., 1999. 19(3): p. 309-19
    [PMID:10476078]
  3. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  4. Hagen G,Guilfoyle T
    Auxin-responsive gene expression: genes, promoters and regulatory factors.
    Plant Mol. Biol., 2002 Jun-Jul. 49(3-4): p. 373-85
    [PMID:12036261]
  5. Tatematsu K, et al.
    MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana.
    Plant Cell, 2004. 16(2): p. 379-93
    [PMID:14729917]
  6. Tian CE, et al.
    Disruption and overexpression of auxin response factor 8 gene of Arabidopsis affect hypocotyl elongation and root growth habit, indicating its possible involvement in auxin homeostasis in light condition.
    Plant J., 2004. 40(3): p. 333-43
    [PMID:15469491]
  7. Okushima Y, et al.
    Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.
    Plant Cell, 2005. 17(2): p. 444-63
    [PMID:15659631]
  8. Wang S,Tiwari SB,Hagen G,Guilfoyle TJ
    AUXIN RESPONSE FACTOR7 restores the expression of auxin-responsive genes in mutant Arabidopsis leaf mesophyll protoplasts.
    Plant Cell, 2005. 17(7): p. 1979-93
    [PMID:15923351]
  9. Nagpal P, et al.
    Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation.
    Development, 2005. 132(18): p. 4107-18
    [PMID:16107481]
  10. Yang JH,Han SJ,Yoon EK,Lee WS
    'Evidence of an auxin signal pathway, microRNA167-ARF8-GH3, and its response to exogenous auxin in cultured rice cells'.
    Nucleic Acids Res., 2006. 34(6): p. 1892-9
    [PMID:16598073]
  11. Ru P,Xu L,Ma H,Huang H
    Plant fertility defects induced by the enhanced expression of microRNA167.
    Cell Res., 2006. 16(5): p. 457-65
    [PMID:16699541]
  12. Goetz M,Vivian-Smith A,Johnson SD,Koltunow AM
    AUXIN RESPONSE FACTOR8 is a negative regulator of fruit initiation in Arabidopsis.
    Plant Cell, 2006. 18(8): p. 1873-86
    [PMID:16829592]
  13. Wu MF,Tian Q,Reed JW
    Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction.
    Development, 2006. 133(21): p. 4211-8
    [PMID:17021043]
  14. Goetz M, et al.
    Expression of aberrant forms of AUXIN RESPONSE FACTOR8 stimulates parthenocarpy in Arabidopsis and tomato.
    Plant Physiol., 2007. 145(2): p. 351-66
    [PMID:17766399]
  15. Gorguet B, et al.
    Mapping and characterization of novel parthenocarpy QTLs in tomato.
    Theor. Appl. Genet., 2008. 116(6): p. 755-67
    [PMID:18231773]
  16. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  17. Swaminathan K,Peterson K,Jack T
    The plant B3 superfamily.
    Trends Plant Sci., 2008. 13(12): p. 647-55
    [PMID:18986826]
  18. Gutierrez L, et al.
    Phenotypic plasticity of adventitious rooting in Arabidopsis is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and microRNA abundance.
    Plant Cell, 2009. 21(10): p. 3119-32
    [PMID:19820192]
  19. Tabata R, et al.
    Arabidopsis auxin response factor6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes.
    Plant Cell Physiol., 2010. 51(1): p. 164-75
    [PMID:20007966]
  20. Jung JH,Lee M,Park CM
    A transcriptional feedback loop modulating signaling crosstalks between auxin and brassinosteroid in Arabidopsis.
    Mol. Cells, 2010. 29(5): p. 449-56
    [PMID:20396969]
  21. Sakata T, et al.
    Auxins reverse plant male sterility caused by high temperatures.
    Proc. Natl. Acad. Sci. U.S.A., 2010. 107(19): p. 8569-74
    [PMID:20421476]
  22. Varaud E, et al.
    AUXIN RESPONSE FACTOR8 regulates Arabidopsis petal growth by interacting with the bHLH transcription factor BIGPETALp.
    Plant Cell, 2011. 23(3): p. 973-83
    [PMID:21421811]
  23. Jay F, et al.
    Misregulation of AUXIN RESPONSE FACTOR 8 underlies the developmental abnormalities caused by three distinct viral silencing suppressors in Arabidopsis.
    PLoS Pathog., 2011. 7(5): p. e1002035
    [PMID:21589905]
  24. Crawford BC,Yanofsky MF
    HALF FILLED promotes reproductive tract development and fertilization efficiency in Arabidopsis thaliana.
    Development, 2011. 138(14): p. 2999-3009
    [PMID:21693516]
  25. Vernoux T, et al.
    The auxin signalling network translates dynamic input into robust patterning at the shoot apex.
    Mol. Syst. Biol., 2011. 7: p. 508
    [PMID:21734647]
  26. Reeves PH, et al.
    A regulatory network for coordinated flower maturation.
    PLoS Genet., 2012. 8(2): p. e1002506
    [PMID:22346763]
  27. Gutierrez L, et al.
    Auxin controls Arabidopsis adventitious root initiation by regulating jasmonic acid homeostasis.
    Plant Cell, 2012. 24(6): p. 2515-27
    [PMID:22730403]
  28. Wang J,Yan DW,Yuan TT,Gao X,Lu YT
    A gain-of-function mutation in IAA8 alters Arabidopsis floral organ development by change of jasmonic acid level.
    Plant Mol. Biol., 2013. 82(1-2): p. 71-83
    [PMID:23483289]
  29. Piya S,Shrestha SK,Binder B,Stewart CN,Hewezi T
    Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis.
    Front Plant Sci, 2014. 5: p. 744
    [PMID:25566309]
  30. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]