PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G28770.2
Common NameAtbZIP63, BZIP63, BZO2H3, T32B20.4
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 314aa    MW: 34311.3 Da    PI: 8.495
Description bZIP family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G28770.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_148.32.2e-15152203455
                  XCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1   4 lkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkke 55 
                  +kr +r+ +NRe+ArrsR+RK+a + eLe+ v +L  eN++L k l   +++
  AT5G28770.2 152 VKRVKRMLSNRESARRSRRRKQAHLSELETQVSQLRVENSKLMKGLTDVTQT 203
                  79****************************************9988777665 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003381.5E-17149213IPR004827Basic-leucine zipper domain
Gene3DG3DSA:1.20.5.1701.4E-11151203No hitNo description
PROSITE profilePS5021710.921151214IPR004827Basic-leucine zipper domain
PfamPF001702.0E-13152199IPR004827Basic-leucine zipper domain
SuperFamilySSF579595.27E-12153202No hitNo description
PROSITE patternPS000360156171IPR004827Basic-leucine zipper domain
PfamPF124983.0E-12220268IPR020983Basic leucine-zipper, C-terminal
PfamPF124982.4E-5280309IPR020983Basic leucine-zipper, C-terminal
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0071215Biological Processcellular response to abscisic acid stimulus
GO:0071333Biological Processcellular response to glucose stimulus
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0042802Molecular Functionidentical protein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0046982Molecular Functionprotein heterodimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 314 aa     Download sequence    Send to blast
MEKVFSDEEI SGNHHWSVNG MTSLNRSASE WAFNRFIQES SAAADDGEST TACGVSVSSP  60
PNVPVDSEEY RAFLKSKLNL ACAAVAMKRG TFIKPQDTSG RSDNGGANES EQASLASSKA  120
TPMMSSAITS GSELSGDEEE ADGETNMNPT NVKRVKRMLS NRESARRSRR RKQAHLSELE  180
TQVSQLRVEN SKLMKGLTDV TQTFNDASVE NRVLKANIET LRAKVKMAEE TVKRLTGFNP  240
MFHNMPQIVS TVSLPSETSN SPDTTSSQVT TPEIISSGNK GKALIGCKMN RTASMRRVES  300
LEHLQKRIRS VGDQ
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1165172RRSRRRKQ
2167172SRRRKQ
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.157540.0leaf| root| seed| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible245925_at0.0
Expression AtlasAT5G28770-
AtGenExpressAT5G28770-
ATTED-IIAT5G28770-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Present in silique valves, vasculature and funiculi. {ECO:0000269|PubMed:18841482}.
UniprotTISSUE SPECIFICITY: Expressed in roots, shoots, young leaves, pollen, and flowers. {ECO:0000269|PubMed:12657652, ECO:0000269|PubMed:18841482}.
Functional Description ? help Back to Top
Source Description
TAIRbZIP protein BZO2H3 mRNA, partial cds
UniProtTranscription factor. {ECO:0000250}.
Function -- GeneRIF ? help Back to Top
  1. Data show that phosphorylation-mimicking serine substitutions strongly interfere with the DNA binding of two prototypical Arabidopsis bZIPs, namely AtZIP63 and HY5.
    [PMID: 20047775]
  2. Data show that AtbZIP1 can bind ACGT-based motifs in vitro and that the binding characteristics appear to be affected by the heterodimerization between AtbZIP1 and the C-group AtbZIPs, including AtbZIP10 and AtbZIP63.
    [PMID: 20080816]
  3. AtbZIP63 is an important node of the glucose-abscisic acid interaction network.
    [PMID: 21844310]
  4. ARR18 interaction negatively interferes with the transcriptional activity of bZIP63 on the PDH1 promoter.
    [PMID: 24948556]
  5. Phosphorylation of bZIP63 by SnRK1 changed its dimerization preference, thereby affecting target gene expression and ultimately primary metabolism.
    [PMID: 26263501]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00040PBM25215497Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G28770.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Strongly repressed by glucose. {ECO:0000269|PubMed:18841482}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G28770, AT5G49450, AT5G58080, AT1G75390
IntActSearch B9DGI8
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G28770
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK3171690.0AK317169.1 Arabidopsis thaliana AT5G28770 mRNA, complete cds, clone: RAFL21-54-H13.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_568508.20.0basic leucine zipper 63
SwissprotB9DGI80.0BZP63_ARATH; Basic leucine zipper 63
TrEMBLD7M6Y60.0D7M6Y6_ARALL; Putative uncharacterized protein
STRINGAT5G28770.20.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP18391639
MalvidsOGEM68462743
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  3. Vincentz M,Bandeira-Kobarg C,Gauer L,Schl
    Evolutionary pattern of angiosperm bZIP factors homologous to the maize Opaque2 regulatory protein.
    J. Mol. Evol., 2003. 56(1): p. 105-16
    [PMID:12569427]
  4. Lara P, et al.
    Synergistic activation of seed storage protein gene expression in Arabidopsis by ABI3 and two bZIPs related to OPAQUE2.
    J. Biol. Chem., 2003. 278(23): p. 21003-11
    [PMID:12657652]
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  6. Satoh R,Fujita Y,Nakashima K,Shinozaki K,Yamaguchi-Shinozaki K
    A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis.
    Plant Cell Physiol., 2004. 45(3): p. 309-17
    [PMID:15047879]
  7. Deppmann CD, et al.
    Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana: a comparison to Homo sapiens B-ZIP motifs.
    Nucleic Acids Res., 2004. 32(11): p. 3435-45
    [PMID:15226410]
  8. Contento AL,Kim SJ,Bassham DC
    Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation.
    Plant Physiol., 2004. 135(4): p. 2330-47
    [PMID:15310832]
  9. Walter M, et al.
    Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
    Plant J., 2004. 40(3): p. 428-38
    [PMID:15469500]
  10. Nawy T, et al.
    Transcriptional profile of the Arabidopsis root quiescent center.
    Plant Cell, 2005. 17(7): p. 1908-25
    [PMID:15937229]
  11. Ehlert A, et al.
    Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors.
    Plant J., 2006. 46(5): p. 890-900
    [PMID:16709202]
  12. Deppmann CD,Alvania RS,Taparowsky EJ
    Cross-species annotation of basic leucine zipper factor interactions: Insight into the evolution of closed interaction networks.
    Mol. Biol. Evol., 2006. 23(8): p. 1480-92
    [PMID:16731568]
  13. Weltmeier F, et al.
    Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors.
    EMBO J., 2006. 25(13): p. 3133-43
    [PMID:16810321]
  14. Kaminaka H, et al.
    bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection.
    EMBO J., 2006. 25(18): p. 4400-11
    [PMID:16957775]
  15. Osuna D, et al.
    Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings.
    Plant J., 2007. 49(3): p. 463-91
    [PMID:17217462]
  16. Peng M,Bi YM,Zhu T,Rothstein SJ
    Genome-wide analysis of Arabidopsis responsive transcriptome to nitrogen limitation and its regulation by the ubiquitin ligase gene NLA.
    Plant Mol. Biol., 2007. 65(6): p. 775-97
    [PMID:17885809]
  17. Usadel B, et al.
    Global transcript levels respond to small changes of the carbon status during progressive exhaustion of carbohydrates in Arabidopsis rosettes.
    Plant Physiol., 2008. 146(4): p. 1834-61
    [PMID:18305208]
  18. Weltmeier F, et al.
    Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and development.
    Plant Mol. Biol., 2009. 69(1-2): p. 107-19
    [PMID:18841482]
  19. Oh SI, et al.
    The Arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5'-methylthioadenosine nucleosidase in a calcium-dependent manner.
    Plant Physiol., 2008. 148(4): p. 1883-96
    [PMID:18945934]
  20. Saez A,Rodrigues A,Santiago J,Rubio S,Rodriguez PL
    HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis.
    Plant Cell, 2008. 20(11): p. 2972-88
    [PMID:19033529]
  21. Kirchler T, et al.
    The role of phosphorylatable serine residues in the DNA-binding domain of Arabidopsis bZIP transcription factors.
    Eur. J. Cell Biol., 2010 Feb-Mar. 89(2-3): p. 175-83
    [PMID:20047775]
  22. Kang SG,Price J,Lin PC,Hong JC,Jang JC
    The arabidopsis bZIP1 transcription factor is involved in sugar signaling, protein networking, and DNA binding.
    Mol Plant, 2010. 3(2): p. 361-73
    [PMID:20080816]
  23. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  24. Matiolli CC, et al.
    The Arabidopsis bZIP gene AtbZIP63 is a sensitive integrator of transient abscisic acid and glucose signals.
    Plant Physiol., 2011. 157(2): p. 692-705
    [PMID:21844310]
  25. Song QX, et al.
    Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants.
    J. Exp. Bot., 2013. 64(14): p. 4329-41
    [PMID:23963672]
  26. Veerabagu M, et al.
    The interaction of the Arabidopsis response regulator ARR18 with bZIP63 mediates the regulation of PROLINE DEHYDROGENASE expression.
    Mol Plant, 2014. 7(10): p. 1560-77
    [PMID:24948556]