PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G16820.2
Common NameATHSF3, ATHSFA1B, F5E19.160, HSF18, HSF3, HSFA1B
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 481aa    MW: 53576.4 Da    PI: 4.5193
Description heat shock factor 3
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G16820.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind110.89.4e-35281202103
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k+y++++d+ ++e++sws+ +nsfvv+   ef+k +LpkyFkh+nf+SFvRQLn+YgF+kv+ ++         weF+++ F +g+k+ll++i 
   AT5G16820.2  28 FLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTYGFRKVDPDR---------WEFANEGFLRGRKQLLKSIV 115
                   9****************************************************************999.........******************** PP

                   XXXXX CS
  HSF_DNA-bind  99 rkkse 103
                   r+k +
   AT5G16820.2 116 RRKPS 120
                   *9875 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF467852.31E-3323117IPR011991Winged helix-turn-helix DNA-binding domain
Gene3DG3DSA:1.10.10.101.3E-3724117IPR011991Winged helix-turn-helix DNA-binding domain
SMARTSM004154.1E-5624117IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004472.7E-3028117IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.0E-192851IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.0E-196678IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS0043406791IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.0E-197991IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009408Biological Processresponse to heat
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020100anatomyhypocotyl
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 481 aa     Download sequence    Send to blast
MESVPESVPS PNSNTPSIPP PVNSVPPFLS KTYDMVDDPL TNEVVSWSSG NNSFVVWSAP  60
EFSKVLLPKY FKHNNFSSFV RQLNTYGFRK VDPDRWEFAN EGFLRGRKQL LKSIVRRKPS  120
HVQQNQQQTQ VQSSSVGACV EVGKFGIEEE VERLKRDKNV LMQELVRLRQ QQQATENQLQ  180
NVGQKVQVME QRQQQMMSFL AKAVQSPGFL NQLVQQNNND GNRQIPGSNK KRRLPVDEQE  240
NRGDNVANGL NRQIVRYQPS INEAAQNMLR QFLNTSTSPR YESVSNNPDS FLLGDVPSST  300
SVDNGNPSSR VSGVTLAEFS PNTVQSATNQ VPEASLAHHP QAGLVQPNIG QSPAQGAAPA  360
DSWSPEFDLV GCETDSGECF DPIMAVLDES EGDAISPEGE GKMNELLEGV PKLPGIQDPF  420
WEQFFSVELP AIADTDDILS GSVENNDLVL EQEPNEWTRN EQQMKYLTEQ MGLLSSEAQR  480
K
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ldu_A1e-242312015123Heat shock factor protein 1
5d5u_B1e-241611718129Heat shock factor protein 1
5d5v_B1e-241611718129Heat shock factor protein 1
5d5v_D1e-241611718129Heat shock factor protein 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.53640.0flower| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible246450_at0.0
Expression AtlasAT5G16820-
AtGenExpressAT5G16820-
ATTED-IIAT5G16820-
Functional Description ? help Back to Top
Source Description
TAIREncodes a putative transcription factor whose expression is not induced by heat but whose stable overexpression leads to expression of HSP. Required early in the stress response for transient expression of heat shock genes.
UniProtTranscriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00514DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G16820.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: DNA-binding capacity is reduced by HSBP in vitro. {ECO:0000269|PubMed:20388662}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G07890(A), AT1G56600(A), AT2G22240(A), AT2G47180(A), AT3G09640(A), AT5G12030(A)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G16820
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G16820
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankY140680.0Y14068.1 Arabidopsis thaliana mRNA for heat shock factor 3.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_197184.20.0heat shock factor 3
RefseqNP_850832.10.0heat shock factor 3
SwissprotO818210.0HFA1B_ARATH; Heat stress transcription factor A-1b
TrEMBLA0A178UCE20.0A0A178UCE2_ARATH; HSFA1B
STRINGAT5G16820.20.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Panchuk II,Volkov RA,Sch
    Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis.
    Plant Physiol., 2002. 129(2): p. 838-53
    [PMID:12068123]
  4. Zhu H, et al.
    Syntenic relationships between Medicago truncatula and Arabidopsis reveal extensive divergence of genome organization.
    Plant Physiol., 2003. 131(3): p. 1018-26
    [PMID:12644654]
  5. Lohmann C,Eggers-Schumacher G,Wunderlich M,Sch
    Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis.
    Mol. Genet. Genomics, 2004. 271(1): p. 11-21
    [PMID:14655047]
  6. Rizhsky L,Davletova S,Liang H,Mittler R
    The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis.
    J. Biol. Chem., 2004. 279(12): p. 11736-43
    [PMID:14722088]
  7. Panikulangara TJ,Eggers-Schumacher G,Wunderlich M,Stransky H,Sch
    Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis.
    Plant Physiol., 2004. 136(2): p. 3148-58
    [PMID:15466240]
  8. Busch W,Wunderlich M,Sch
    Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana.
    Plant J., 2005. 41(1): p. 1-14
    [PMID:15610345]
  9. Nishizawa A, et al.
    Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress.
    Plant J., 2006. 48(4): p. 535-47
    [PMID:17059409]
  10. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  11. Nishizawa A,Yabuta Y,Shigeoka S
    Galactinol and raffinose constitute a novel function to protect plants from oxidative damage.
    Plant Physiol., 2008. 147(3): p. 1251-63
    [PMID:18502973]
  12. Li M, et al.
    Detection of in vivo interactions between Arabidopsis class A-HSFs, using a novel BiFC fragment, and identification of novel class B-HSF interacting proteins.
    Eur. J. Cell Biol., 2010 Feb-Mar. 89(2-3): p. 126-32
    [PMID:19945192]
  13. Banti V,Mafessoni F,Loreti E,Alpi A,Perata P
    The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis.
    Plant Physiol., 2010. 152(3): p. 1471-83
    [PMID:20089772]
  14. Xu X, et al.
    Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress.
    Biotechnol. Lett., 2010. 32(7): p. 979-87
    [PMID:20229063]
  15. Hsu SF,Lai HC,Jinn TL
    Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis.
    Plant Physiol., 2010. 153(2): p. 773-84
    [PMID:20388662]
  16. Li M,Berendzen KW,Sch
    Promoter specificity and interactions between early and late Arabidopsis heat shock factors.
    Plant Mol. Biol., 2010. 73(4-5): p. 559-67
    [PMID:20458611]
  17. Hsu SF,Jinn TL
    AtHSBP functions in seed development and the motif is required for subcellular localization and interaction with AtHSFs.
    Plant Signal Behav, 2010. 5(8): p. 1042-4
    [PMID:20657173]
  18. Yoshida T, et al.
    Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression.
    Mol. Genet. Genomics, 2011. 286(5-6): p. 321-32
    [PMID:21931939]
  19. Liu HC,Charng YY
    Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response.
    Plant Signal Behav, 2012. 7(5): p. 547-50
    [PMID:22516818]
  20. Guan Q,Lu X,Zeng H,Zhang Y,Zhu J
    Heat stress induction of miR398 triggers a regulatory loop that is critical for thermotolerance in Arabidopsis.
    Plant J., 2013. 74(5): p. 840-51
    [PMID:23480361]
  21. Bechtold U, et al.
    Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection.
    J. Exp. Bot., 2013. 64(11): p. 3467-81
    [PMID:23828547]
  22. Guan Q,Yue X,Zeng H,Zhu J
    The protein phosphatase RCF2 and its interacting partner NAC019 are critical for heat stress-responsive gene regulation and thermotolerance in Arabidopsis.
    Plant Cell, 2014. 26(1): p. 438-53
    [PMID:24415771]
  23. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  24. Prändl R,Hinderhofer K,Eggers-Schumacher G,Schöffl F
    HSF3, a new heat shock factor from Arabidopsis thaliana, derepresses the heat shock response and confers thermotolerance when overexpressed in transgenic plants.
    Mol. Gen. Genet., 1998. 258(3): p. 269-78
    [PMID:9645433]