PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G13330.1
Common NameERF113, Rap2.6L, T31B5.150
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 212aa    MW: 24287.7 Da    PI: 9.1153
Description related to AP2 6l
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G13330.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP264.81.7e-203887255
          AP2  2 gykGVrwdkkrgrWvAeIrdpsengkr.krfslgkfgtaeeAakaaiaarkkleg 55
                 +y+GVr+++ +g+W+AeIrdp+    + +r++lg+f taeeAa a+++a++k++g
  AT5G13330.1 38 HYRGVRQRP-WGKWAAEIRDPK----KaARVWLGTFETAEEAALAYDRAALKFKG 87
                 7********.**********94....45************************987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:3.30.730.103.4E-333796IPR001471AP2/ERF domain
SMARTSM003802.6E-3838101IPR001471AP2/ERF domain
SuperFamilySSF541715.36E-233896IPR016177DNA-binding domain
CDDcd000181.06E-333896No hitNo description
PfamPF008472.4E-143887IPR001471AP2/ERF domain
PROSITE profilePS5103224.9743895IPR001471AP2/ERF domain
PRINTSPR003674.0E-123950IPR001471AP2/ERF domain
PRINTSPR003674.0E-126177IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009414Biological Processresponse to water deprivation
GO:0009651Biological Processresponse to salt stress
GO:0009723Biological Processresponse to ethylene
GO:0009737Biological Processresponse to abscisic acid
GO:0009751Biological Processresponse to salicylic acid
GO:0009753Biological Processresponse to jasmonic acid
GO:0009873Biological Processethylene-activated signaling pathway
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0071497Biological Processcellular response to freezing
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001185developmental stageplant embryo globular stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 212 aa     Download sequence    Send to blast
MVSALSRVIE NPTDPPVKQE LDKSDQHQPD QDQPRRRHYR GVRQRPWGKW AAEIRDPKKA  60
ARVWLGTFET AEEAALAYDR AALKFKGTKA KLNFPERVQG PTTTTTISHA PRGVSESMNS  120
PPPRPGPPST TTTSWPMTYN QDILQYAQLL TSNNEVDLSY YTSTLFSQPF STPSSSSSSS  180
QQTQQQQLQQ QQQQREEEEK NYGYNYYNYP RE
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A2e-273798163ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A2e-273798466ATERF1
3gcc_A2e-273798466ATERF1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.320830.0cell culture| flower
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453579620.0
Genevisible250287_at0.0
Expression AtlasAT5G13330-
AtGenExpressAT5G13330-
ATTED-IIAT5G13330-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in pollen grains. {ECO:0000269|PubMed:21069430}.
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-4 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 7 members in this subfamily.
UniProtTranscriptional activator involved in the regulation of plant development and tolerance to abiotic stresses (PubMed:21069430). Acts as positive regulator of tolerance to waterlogging stress. Delays waterlogging-induced premature senescence by regulating stomatal closure and antioxidant enzyme activity. May function through ABI1-mediated abscisic acid (ABA) signaling pathway (PubMed:22661072). Involved in tissue reunion of wounded inflorescence stems. Required for the division of pith cells in the reunion process, which is dependent on polar-transported auxin and the wound-inducible hormones ethylene and jasmonate (PubMed:21911380). Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:21069430, ECO:0000269|PubMed:21911380, ECO:0000269|PubMed:22661072}.
Function -- GeneRIF ? help Back to Top
  1. Data demonstrate that RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340) are transactivators, they exhibit tissue specific expression, and participate in developmental processes as well as biotic/abiotic stress signaling.
    [PMID: 21069430]
  2. Data show that two plant-specific transcription factor genes, ANAC071 (At4g17980) and RAP2.6L (At5g13330) were abundantly expressed.
    [PMID: 21911380]
  3. These results suggest that RAP2.6L overexpression delays waterlogging induced premature senescence and might function through ABI1-mediated abscissic acid signaling pathway.
    [PMID: 22661072]
  4. Results suggest that jasmonic acid (JA) and RAP2.6L, induced by grafting, are not necessary for cell proliferation process in healing.
    [PMID: 29344830]
  5. RAP2.6L expression is regulated by AMP1.
    [PMID: 29884678]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G13330.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by salt stress (PubMed:16133218, PubMed:21069430). Induced drought stress, jasmonate (JA), salicylic acid (SA), abscisic acid (ABA) and ethylene. Down-regulated by freezing stress (PubMed:21069430). Induced by wounding in the flowering stem (PubMed:21911380). Induced by waterlogging. {ECO:0000269|PubMed:16133218, ECO:0000269|PubMed:21069430, ECO:0000269|PubMed:21911380}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9LYU3
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G13330
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY5608610.0AY560861.1 Arabidopsis thaliana putative AP2/EREBP transcription factor (At5g13330) mRNA, complete cds.
GenBankBT0116090.0BT011609.1 Arabidopsis thaliana At5g13330 mRNA, complete cds.
GenBankBT0122500.0BT012250.1 Arabidopsis thaliana At5g13330 gene, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_196837.11e-155related to AP2 6l
SwissprotQ9LYU31e-156EF113_ARATH; Ethylene-responsive transcription factor ERF113
TrEMBLA0A178UKL81e-154A0A178UKL8_ARATH; Rap2.6L
STRINGAT5G13330.11e-155(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM10281650
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Sun K,Cui Y,Hauser BA
    Environmental stress alters genes expression and induces ovule abortion: reactive oxygen species appear as ovules commit to abort.
    Planta, 2005. 222(4): p. 632-42
    [PMID:16133218]
  3. Brenner WG,Romanov GA,K
    Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades.
    Plant J., 2005. 44(2): p. 314-33
    [PMID:16212609]
  4. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  5. Cai S,Lashbrook CC
    Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2.
    Plant Physiol., 2008. 146(3): p. 1305-21
    [PMID:18192438]
  6. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  7. Krishnaswamy SS, et al.
    Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana.
    BMC Plant Biol., 2008. 8: p. 91
    [PMID:18783601]
  8. Van Leene J, et al.
    Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.
    Mol. Syst. Biol., 2010. 6: p. 397
    [PMID:20706207]
  9. Krishnaswamy S,Verma S,Rahman MH,Kav NN
    Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis.
    Plant Mol. Biol., 2011. 75(1-2): p. 107-27
    [PMID:21069430]
  10. Asahina M, et al.
    Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2011. 108(38): p. 16128-32
    [PMID:21911380]
  11. Liu P,Sun F,Gao R,Dong H
    RAP2.6L overexpression delays waterlogging induced premature senescence by increasing stomatal closure more than antioxidant enzyme activity.
    Plant Mol. Biol., 2012. 79(6): p. 609-22
    [PMID:22661072]
  12. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  13. Huang Z,Zhao P,Medina J,Meilan R,Woeste K
    Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling.
    PLoS ONE, 2013. 8(11): p. e75857
    [PMID:24265672]
  14. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  15. Asahina M,Satoh S
    Molecular and physiological mechanisms regulating tissue reunion in incised plant tissues.
    J. Plant Res., 2015. 128(3): p. 381-8
    [PMID:25736731]
  16. Hickman R, et al.
    Architecture and Dynamics of the Jasmonic Acid Gene Regulatory Network.
    Plant Cell, 2017. 29(9): p. 2086-2105
    [PMID:28827376]
  17. Li B, et al.
    Network-Guided Discovery of Extensive Epistasis between Transcription Factors Involved in Aliphatic Glucosinolate Biosynthesis.
    Plant Cell, 2018. 30(1): p. 178-195
    [PMID:29317470]
  18. Matsuoka K, et al.
    RAP2.6L and jasmonic acid-responsive genes are expressed upon Arabidopsis hypocotyl grafting but are not needed for cell proliferation related to healing.
    Plant Mol. Biol., 2018. 96(6): p. 531-542
    [PMID:29344830]
  19. Yang S,Poretska O,Sieberer T
    ALTERED MERISTEM PROGRAM1 Restricts Shoot Meristem Proliferation and Regeneration by Limiting HD-ZIP III-Mediated Expression of RAP2.6L.
    Plant Physiol., 2018. 177(4): p. 1580-1594
    [PMID:29884678]
  20. Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD
    The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81
    [PMID:9192694]