PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G06960.1
Common NameBZIP26, MOJ9.13, OBF5, TGA5
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 330aa    MW: 36890.4 Da    PI: 8.9213
Description OCS-element binding factor 5
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G06960.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_130.76.6e-104586445
                 XCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1  4 lkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaL 45
                  k  rr+++NReAAr+sR+RKka++++Le+   +L++  ++L
  AT5G06960.1 45 QKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQEL 86
                 5888*************************9877777766555 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.20.5.1709.3E-83989No hitNo description
SMARTSM003381.9E-742105IPR004827Basic-leucine zipper domain
PROSITE profilePS502179.4384488IPR004827Basic-leucine zipper domain
PfamPF001706.4E-74686IPR004827Basic-leucine zipper domain
SuperFamilySSF579595.56E-74689No hitNo description
PROSITE patternPS0003604964IPR004827Basic-leucine zipper domain
PfamPF141442.7E-32128204IPR025422Transcription factor TGA like domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009410Biological Processresponse to xenobiotic stimulus
GO:0009814Biological Processdefense response, incompatible interaction
GO:0009863Biological Processsalicylic acid mediated signaling pathway
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0042802Molecular Functionidentical protein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 330 aa     Download sequence    Send to blast
MGDTSPRTSV STDGDTDHNN LMFDEGHLGI GASDSSDRSK SKMDQKTLRR LAQNREAARK  60
SRLRKKAYVQ QLENSRLKLT QLEQELQRAR QQGVFISSSG DQAHSTAGDG AMAFDVEYRR  120
WQEDKNRQMK ELSSAIDSHA TDSELRIIVD GVIAHYEELY RIKGNAAKSD VFHLLSGMWK  180
TPAERCFLWL GGFRSSELLK LIASQLEPLT EQQSLDINNL QQSSQQAEDA LSQGMDNLQQ  240
SLADTLSSGT LGSSSSGNVA SYMGQMAMAM GKLGTLEGFI RQADNLRLQT YQQMVRLLTT  300
RQSARALLAV HNYTLRLRAL SSLWLARPRE
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.54080.0flower| root| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453577260.0
Genevisible250655_at0.0
Expression AtlasAT5G06960-
AtGenExpressAT5G06960-
ATTED-IIAT5G06960-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Predominantly expressed in roots.
Functional Description ? help Back to Top
Source Description
TAIREncodes a basic leucine zipper (B-ZIP) containing protein that interacts with NPR1 to promote expression of salicylic acid induced genes. Binds the ocs-element.
UniProtTranscriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.
Function -- GeneRIF ? help Back to Top
  1. data show TGA factors, which are essential for the salicylic acid (SA)-dependent establishment of systemic acquired resistance, play a pivotal role in the activation of the jasmonic acid/ethylene pathway, both after pathogen infection & hormone treatment
    [PMID: 19832945]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00493DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G06960.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G14610(A), AT5G44420(R)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDsalicylic acid
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G06960
IntActSearch Q39163
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G06960
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0588300.0AY058830.1 Arabidopsis thaliana AT5g06960/MOJ9_13 mRNA, complete cds.
GenBankAY1438640.0AY143864.1 Arabidopsis thaliana At5g06960/MOJ9_13 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_196313.10.0OCS-element binding factor 5
RefseqNP_974745.10.0OCS-element binding factor 5
SwissprotQ391630.0TGA5_ARATH; Transcription factor TGA5
TrEMBLA0A178UIF00.0A0A178UIF0_ARATH; TGA5
STRINGAT5G06960.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM66728133
Representative plantOGRP7221667
Publications ? help Back to Top
  1. Zhang Y,Fan W,Kinkema M,Li X,Dong X
    Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.
    Proc. Natl. Acad. Sci. U.S.A., 1999. 96(11): p. 6523-8
    [PMID:10339621]
  2. Zhou JM, et al.
    NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid.
    Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202
    [PMID:10659709]
  3. Després C,DeLong C,Glaze S,Liu E,Fobert PR
    The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors.
    Plant Cell, 2000. 12(2): p. 279-90
    [PMID:10662863]
  4. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  5. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  6. Kim HS,Delaney TP
    Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica.
    Plant J., 2002. 32(2): p. 151-63
    [PMID:12383081]
  7. Zhang Y,Tessaro MJ,Lassner M,Li X
    Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance.
    Plant Cell, 2003. 15(11): p. 2647-53
    [PMID:14576289]
  8. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  9. Liu G,Holub EB,Alonso JM,Ecker JR,Fobert PR
    An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.
    Plant J., 2005. 41(2): p. 304-18
    [PMID:15634206]
  10. Kang HG,Klessig DF
    Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2.
    Plant Mol. Biol., 2005. 57(4): p. 541-57
    [PMID:15821979]
  11. Zhang Y, et al.
    Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs.
    Plant J., 2006. 48(5): p. 647-56
    [PMID:17076807]
  12. Churchman ML, et al.
    SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana.
    Plant Cell, 2006. 18(11): p. 3145-57
    [PMID:17098811]
  13. Rochon A,Boyle P,Wignes T,Fobert PR,Després C
    The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines.
    Plant Cell, 2006. 18(12): p. 3670-85
    [PMID:17172357]
  14. Ndamukong I, et al.
    SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription.
    Plant J., 2007. 50(1): p. 128-39
    [PMID:17397508]
  15. Mueller S, et al.
    General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis.
    Plant Cell, 2008. 20(3): p. 768-85
    [PMID:18334669]
  16. Manzano C,Abraham Z,L
    Identification of ubiquitinated proteins in Arabidopsis.
    Plant Mol. Biol., 2008. 68(1-2): p. 145-58
    [PMID:18535787]
  17. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  18. Fode B,Siemsen T,Thurow C,Weigel R,Gatz C
    The Arabidopsis GRAS protein SCL14 interacts with class II TGA transcription factors and is essential for the activation of stress-inducible promoters.
    Plant Cell, 2008. 20(11): p. 3122-35
    [PMID:18984675]
  19. Li S, et al.
    Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana.
    Plant Cell, 2009. 21(2): p. 429-41
    [PMID:19218396]
  20. Zander M,La Camera S,Lamotte O,Métraux JP,Gatz C
    Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses.
    Plant J., 2010. 61(2): p. 200-10
    [PMID:19832945]
  21. Matthes MC, et al.
    The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence.
    Planta, 2010. 232(5): p. 1163-80
    [PMID:20711606]
  22. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  23. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
    [PMID:22065421]
  24. Zander M,Chen S,Imkampe J,Thurow C,Gatz C
    Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interacting glutaredoxins depends on their C-terminal ALWL motif.
    Mol Plant, 2012. 5(4): p. 831-40
    [PMID:22207719]
  25. Heyndrickx KS,Vandepoele K
    Systematic identification of functional plant modules through the integration of complementary data sources.
    Plant Physiol., 2012. 159(3): p. 884-901
    [PMID:22589469]
  26. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  27. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  28. Zander M,Thurow C,Gatz C
    TGA Transcription Factors Activate the Salicylic Acid-Suppressible Branch of the Ethylene-Induced Defense Program by Regulating ORA59 Expression.
    Plant Physiol., 2014. 165(4): p. 1671-1683
    [PMID:24989234]
  29. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  30. Walper E,Weiste C,Mueller MJ,Hamberg M,Dröge-Laser W
    Screen Identifying Arabidopsis Transcription Factors Involved in the Response to 9-Lipoxygenase-Derived Oxylipins.
    PLoS ONE, 2016. 11(4): p. e0153216
    [PMID:27073862]
  31. Huang LJ, et al.
    Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana.
    BMC Plant Biol., 2016. 16(1): p. 200
    [PMID:27624344]
  32. Lam E,Lam YK
    Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity.
    Nucleic Acids Res., 1995. 23(18): p. 3778-85
    [PMID:7479010]
  33. Zhang B,Foley RC,Singh KB
    Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins.
    Plant J., 1993. 4(4): p. 711-6
    [PMID:8252072]
  34. Zhang B,Chen W,Foley RC,Büttner M,Singh KB
    Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences.
    Plant Cell, 1995. 7(12): p. 2241-52
    [PMID:8718629]
  35. Xiang C,Miao Z,Lam E
    DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.
    Plant Mol. Biol., 1997. 34(3): p. 403-15
    [PMID:9225852]