PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G03720.1
Common NameAT-HSFA3, F17C15.140, HSF17, HSFA3
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 412aa    MW: 46458.1 Da    PI: 5.131
Description heat shock transcription factor A3
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G03720.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind106.42.3e-33561472102
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k++++++d++l+ +isw  +g sfvv+d+ efa+ +Lp+ Fkh+nf+SFvRQLn+YgF+k+++++         weF+++ F +gkk+ll++i+
   AT5G03720.1  56 FLSKTFDLVDDPTLDPVISWGLTGASFVVWDPLEFARIILPRNFKHNNFSSFVRQLNTYGFRKIDTDK---------WEFANEAFLRGKKHLLKNIH 143
                   9****************************************************************999.........******************** PP

                   XXXX CS
  HSF_DNA-bind  99 rkks 102
                   r++s
   AT5G03720.1 144 RRRS 147
                   *987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF467855.85E-3351145IPR011991Winged helix-turn-helix DNA-binding domain
SMARTSM004155.9E-5552145IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene3DG3DSA:1.10.10.103.2E-3553145IPR011991Winged helix-turn-helix DNA-binding domain
PRINTSPR000562.1E-175679IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004472.6E-2956145IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.1E-1794106IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS00434095119IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.1E-17107119IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009408Biological Processresponse to heat
GO:0010200Biological Processresponse to chitin
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000293anatomyguard cell
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 412 aa     Download sequence    Send to blast
MSPKKDAVSK PTPISVPVSR RSDIPGSLYV DTDMGFSGSP LPMPLDILQG NPIPPFLSKT  60
FDLVDDPTLD PVISWGLTGA SFVVWDPLEF ARIILPRNFK HNNFSSFVRQ LNTYGFRKID  120
TDKWEFANEA FLRGKKHLLK NIHRRRSPQS NQTCCSSTSQ SQGSPTEVGG EIEKLRKERR  180
ALMEEMVELQ QQSRGTARHV DTVNQRLKAA EQRQKQLLSF LAKLFQNRGF LERLKNFKGK  240
EKGGALGLEK ARKKFIKHHQ QPQDSPTGGE VVKYEADDWE RLLMYDEETE NTKGLGGMTS  300
SDPKGKNLMY PSEEEMSKPD YLMSFPSPEG LIKQEETTWS MGFDTTIPSF SNTDAWGNTM  360
DYNDVSEFGF AAETTSDGLP DVCWEQFAAG ITETGFNWPT GDDDDNTPMN DP
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ldu_A7e-245315017125Heat shock factor protein 1
5d5u_B8e-245314526129Heat shock factor protein 1
5d5v_B8e-245314526129Heat shock factor protein 1
5d5v_D8e-245314526129Heat shock factor protein 1
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453576040.0
Genevisible250910_at0.0
Expression AtlasAT5G03720-
AtGenExpressAT5G03720-
ATTED-IIAT5G03720-
Functional Description ? help Back to Top
Source Description
TAIRMember of Heat Stress Transcription Factor (Hsf) family. Expression is regulated by DREB2A and in turn HSFA3 regulates the expression of hsps Hsp18.1-CI and Hsp26.5-MII35S. Involved in establishing thermotolerence.
UniProtTranscriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress response. Activated by DREB2A under heat stress. {ECO:0000269|PubMed:17999647, ECO:0000269|PubMed:18261981}.
Function -- GeneRIF ? help Back to Top
  1. Altogether these data demonstrate that HsfA3 is transcriptionally controlled by DREB2A and important for the establishment of thermotolerance.
    [PMID: 17999647]
  2. These results indicate that HsfA3 regulates expression of many heat-inducible genes in the transcriptional cascade downstream of the DREB2A stress-regulatory system and functions in acquisition of thermotolerance under the control of the DREB2A cascade.
    [PMID: 18261981]
  3. these results indicate that DREB2C and HsfA3 are key players in regulating the heat tolerance of Arabidopsis.
    [PMID: 20849812]
  4. Findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis.
    [PMID: 27109422]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G03720.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By heat stress. {ECO:0000269|PubMed:17999647, ECO:0000269|PubMed:18261981}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G03720
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK1173180.0AK117318.1 Arabidopsis thaliana At5g03720 mRNA for putative heat shock transcription factor, complete cds, clone: RAFL16-88-H09.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001318473.10.0heat shock transcription factor A3
RefseqNP_195992.20.0heat shock transcription factor A3
SwissprotQ8GYY10.0HSFA3_ARATH; Heat stress transcription factor A-3
TrEMBLA0A178UHR40.0A0A178UHR4_ARATH; HSFA3
STRINGAT5G03720.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM109502529
Representative plantOGRP10024911
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
    [PMID:11910074]
  4. Qin F, et al.
    Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L.
    Plant J., 2007. 50(1): p. 54-69
    [PMID:17346263]
  5. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  6. Schramm F, et al.
    A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis.
    Plant J., 2008. 53(2): p. 264-74
    [PMID:17999647]
  7. Larkindale J,Vierling E
    Core genome responses involved in acclimation to high temperature.
    Plant Physiol., 2008. 146(2): p. 748-61
    [PMID:18055584]
  8. Yoshida T, et al.
    Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system.
    Biochem. Biophys. Res. Commun., 2008. 368(3): p. 515-21
    [PMID:18261981]
  9. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  10. Chen H, et al.
    Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response.
    Biochem. Biophys. Res. Commun., 2010. 401(2): p. 238-44
    [PMID:20849812]
  11. Hwang JE, et al.
    Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress.
    Mol. Cells, 2012. 33(2): p. 135-40
    [PMID:22286229]
  12. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  13. Jung HS, et al.
    Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light.
    Proc. Natl. Acad. Sci. U.S.A., 2013. 110(35): p. 14474-9
    [PMID:23918368]
  14. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  15. Nie S,Yue H,Xing D
    A Potential Role for Mitochondrial Produced Reactive Oxygen Species in Salicylic Acid-Mediated Plant Acquired Thermotolerance.
    Plant Physiol., 2016.
    [PMID:26099269]
  16. Hu Z, et al.
    Histone acetyltransferase GCN5 is essential for heat stress-responsive gene activation and thermotolerance in Arabidopsis.
    Plant J., 2015. 84(6): p. 1178-91
    [PMID:26576681]
  17. Song C,Chung WS,Lim CO
    Overexpression of Heat Shock Factor Gene HsfA3 Increases Galactinol Levels and Oxidative Stress Tolerance in Arabidopsis.
    Mol. Cells, 2016. 39(6): p. 477-83
    [PMID:27109422]
  18. Kim GD,Cho YH,Lee BH,Yoo SD
    STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance.
    Plant Physiol., 2017. 173(4): p. 2370-2382
    [PMID:28223317]
  19. Song C,Lee J,Kim T,Hong JC,Lim CO
    VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis.
    Planta, 2018. 247(6): p. 1439-1448
    [PMID:29536220]