PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G36730.1
Common NameAtGBF1, BZIP41, C7A10_630, GBF1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 315aa    MW: 33932.3 Da    PI: 6.3772
Description G-box binding factor 1
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G36730.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_178.11.1e-24220282163
                  XXXXCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1   1 ekelkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkevaklksev 63 
                  e+elkr++rkq+NRe+ArrsR+RK+ae+e+L+++v++L++eN++L++el++l+ e++klkse+
  AT4G36730.1 220 ERELKRQKRKQSNRESARRSRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSEN 282
                  89***********************************************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF077774.7E-30194IPR012900G-box binding protein, multifunctional mosaic region
Gene3DG3DSA:1.20.5.1703.1E-19217280No hitNo description
PfamPF001701.1E-22220282IPR004827Basic-leucine zipper domain
SMARTSM003383.8E-23220284IPR004827Basic-leucine zipper domain
PROSITE profilePS5021713.817222285IPR004827Basic-leucine zipper domain
SuperFamilySSF579594.22E-12223280No hitNo description
CDDcd147025.54E-24225276No hitNo description
PROSITE patternPS000360227242IPR004827Basic-leucine zipper domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010310Biological Processregulation of hydrogen peroxide metabolic process
GO:0010629Biological Processnegative regulation of gene expression
GO:0090342Biological Processregulation of cell aging
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 315 aa     Download sequence    Send to blast
MGTSEDKMPF KTTKPTSSAQ EVPPTPYPDW QNSMQAYYGG GGTPNPFFPS PVGSPSPHPY  60
MWGAQHHMMP PYGTPVPYPA MYPPGAVYAH PSMPMPPNSG PTNKEPAKDQ ASGKKSKGNS  120
KKKAEGGDKA LSGSGNDGAS HSDESVTAGS SDENDENANQ QEQGSIRKPS FGQMLADASS  180
QSTTGEIQGS VPMKPVAPGT NLNIGMDLWS SQAGVPVKDE RELKRQKRKQ SNRESARRSR  240
LRKQAECEQL QQRVESLSNE NQSLRDELQR LSSECDKLKS ENNSIQDELQ RVLGAEAVAN  300
LEQNAAGSKD GEGTN
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1236242RRSRLRK
2236243RRSRLRKQ
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.620.0flower| leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453543670.0
Genevisible246211_at0.0
Expression AtlasAT4G36730-
AtGenExpressAT4G36730-
ATTED-IIAT4G36730-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Found in both light and dark grown leaves.
Functional Description ? help Back to Top
Source Description
TAIRmember of a gene family encoding basic leucine zipper proteins (GBFs) which bind the G-box
UniProtBinds to the G-box motif (5'-CCACGTGG-3') of the rbcS-1A gene promoter (PubMed:1373374). G-box and G-box-like motifs are cis-acting elements defined in promoters of certain plant genes which are regulated by such diverse stimuli as light-induction or hormone control. Binds to the G-box motif 5'-CACGTG-3' of LHCB2.4 (At3g27690) promoter. May act as transcriptional activator in light-regulated expression of LHCB2.4. Probably binds DNA as monomer. DNA-binding activity is redox-dependent (PubMed:22718771). {ECO:0000269|PubMed:1373374, ECO:0000269|PubMed:22718771}.
Function -- GeneRIF ? help Back to Top
  1. GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1
    [PMID: 18930926]
  2. In Arabidopsis, GBF1-related exchange factors for the ARF GTPases (ARF GEFs) GNOM and GNL2 play essential roles in polar tip growth of root hairs and pollen, respectively.
    [PMID: 22138577]
  3. the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development.
    [PMID: 22692212]
  4. Genetic interactions of HY1 and GBF1 in seedling photomorphogenesis.
    [PMID: 22766018]
  5. The DNA binding activity of GBF1 is modulated by heterodimeriation with HY5 and HYH.
    [PMID: 24157608]
  6. GBF1 is a transcriptional repressor of the AtCathB3 gene and affects germination kinetics of A. thaliana seeds. As AtCathB3 is also expressed during post-germination in cotyledons, a role for the AtCathB3-like protease in reserve mobilization is inferred.
    [PMID: 24600022]
  7. This study reveals that MYC2 and GBF1 colocalize and physically interact in the nucleus. This interaction requires the N-terminal domain of each protein.
    [PMID: 26047210]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00039PBM25215497Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G36730.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G29930(A), AT4G10040(A), AT4G35090(R)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G11260, AT5G44190, AT1G32640
IntActSearch P42774
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G36730
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ8432570.0AJ843257.1 Arabidopsis thaliana mRNA for Z-box binding factor 2 protein (ZBF2 gene).
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_195391.10.0G-box binding factor 1
SwissprotP427740.0GBF1_ARATH; G-box-binding factor 1
TrEMBLA0A384KK130.0A0A384KK13_ARATH; GBF1
TrEMBLQ5K1L60.0Q5K1L6_ARATH; Z-box binding factor 2 protein
STRINGAT4G36730.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM72742643
Representative plantOGRP7001767
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Tamai H,Iwabuchi M,Meshi T
    Arabidopsis GARP transcriptional activators interact with the Pro-rich activation domain shared by G-box-binding bZIP factors.
    Plant Cell Physiol., 2002. 43(1): p. 99-107
    [PMID:11828027]
  3. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  4. Schindler U,Menkens AE,Beckmann H,Ecker JR,Cashmore AR
    Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins.
    EMBO J., 1992. 11(4): p. 1261-73
    [PMID:1373374]
  5. Schindler U,Beckmann H,Cashmore AR
    TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG.
    Plant Cell, 1992. 4(10): p. 1309-19
    [PMID:1446171]
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  7. Klimczak LJ,Schindler U,Cashmore AR
    DNA binding activity of the Arabidopsis G-box binding factor GBF1 is stimulated by phosphorylation by casein kinase II from broccoli.
    Plant Cell, 1992. 4(1): p. 87-98
    [PMID:1525562]
  8. Schindler U,Terzaghi W,Beckmann H,Kadesch T,Cashmore AR
    DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1.
    EMBO J., 1992. 11(4): p. 1275-89
    [PMID:1563344]
  9. Sehnke PC,Laughner BJ,Lyerly Linebarger CR,Gurley WB,Ferl RJ
    Identification and characterization of GIP1, an Arabidopsis thaliana protein that enhances the DNA binding affinity and reduces the oligomeric state of G-box binding factors.
    Cell Res., 2005. 15(8): p. 567-75
    [PMID:16117846]
  10. Mallappa C,Yadav V,Negi P,Chattopadhyay S
    A basic leucine zipper transcription factor, G-box-binding factor 1, regulates blue light-mediated photomorphogenic growth in Arabidopsis.
    J. Biol. Chem., 2006. 281(31): p. 22190-9
    [PMID:16638747]
  11. Popescu SC, et al.
    Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(11): p. 4730-5
    [PMID:17360592]
  12. Shen H,Cao K,Wang X
    AtbZIP16 and AtbZIP68, two new members of GBFs, can interact with other G group bZIPs in Arabidopsis thaliana.
    BMB Rep, 2008. 41(2): p. 132-8
    [PMID:18315949]
  13. Mallappa C,Singh A,Ram H,Chattopadhyay S
    GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1.
    J. Biol. Chem., 2008. 283(51): p. 35772-82
    [PMID:18930926]
  14. Welchen E, et al.
    A segment containing a G-box and an ACGT motif confers differential expression characteristics and responses to the Arabidopsis Cytc-2 gene, encoding an isoform of cytochrome c.
    J. Exp. Bot., 2009. 60(3): p. 829-45
    [PMID:19098132]
  15. Nozawa A, et al.
    Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.
    Biosci. Biotechnol. Biochem., 2009. 73(7): p. 1661-4
    [PMID:19584551]
  16. Smykowski A,Zimmermann P,Zentgraf U
    G-Box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis.
    Plant Physiol., 2010. 153(3): p. 1321-31
    [PMID:20484024]
  17. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
    [PMID:22037706]
  18. Richter S, et al.
    Polarized cell growth in Arabidopsis requires endosomal recycling mediated by GBF1-related ARF exchange factors.
    Nat. Cell Biol., 2012. 14(1): p. 80-6
    [PMID:22138577]
  19. Singh A,Ram H,Abbas N,Chattopadhyay S
    Molecular interactions of GBF1 with HY5 and HYH proteins during light-mediated seedling development in Arabidopsis thaliana.
    J. Biol. Chem., 2012. 287(31): p. 25995-6009
    [PMID:22692212]
  20. Shaikhali J, et al.
    Redox-mediated mechanisms regulate DNA binding activity of the G-group of basic region leucine zipper (bZIP) transcription factors in Arabidopsis.
    J. Biol. Chem., 2012. 287(33): p. 27510-25
    [PMID:22718771]
  21. Prasad VB,Gupta N,Nandi A,Chattopadhyay S
    HY1 genetically interacts with GBF1 and regulates the activity of the Z-box containing promoters in light signaling pathways in Arabidopsis thaliana.
    Mech. Dev., 2012 Sep-Dec. 129(9-12): p. 298-307
    [PMID:22766018]
  22. Ram H,Chattopadhyay S
    Molecular interaction of bZIP domains of GBF1, HY5 and HYH in Arabidopsis seedling development.
    Plant Signal Behav, 2013. 8(1): p. e22703
    [PMID:23123453]
  23. Gangappa SN,Srivastava AK,Maurya JP,Ram H,Chattopadhyay S
    Z-box binding transcription factors (ZBFs): a new class of transcription factors in Arabidopsis seedling development.
    Mol Plant, 2013. 6(6): p. 1758-68
    [PMID:24157607]
  24. Ram H,Priya P,Jain M,Chattopadhyay S
    Genome-wide DNA binding of GBF1 is modulated by its heterodimerizing protein partners, HY5 and HYH.
    Mol Plant, 2014. 7(2): p. 448-51
    [PMID:24157608]
  25. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  26. Iglesias-Fern
    The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.
    J. Exp. Bot., 2014. 65(8): p. 2009-21
    [PMID:24600022]
  27. Shaikhali J
    GIP1 protein is a novel cofactor that regulates DNA-binding affinity of redox-regulated members of bZIP transcription factors involved in the early stages of Arabidopsis development.
    Protoplasma, 2015. 252(3): p. 867-83
    [PMID:25387999]
  28. Karumuri S,Bandopadhyay R
    In silico analysis of the structure and interaction of COP1 protein of Arabidopsis thaliana.
    Indian J. Biochem. Biophys., 2014. 51(5): p. 343-9
    [PMID:25630103]
  29. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  30. Maurya JP,Sethi V,Gangappa SN,Gupta N,Chattopadhyay S
    Interaction of MYC2 and GBF1 results in functional antagonism in blue light-mediated Arabidopsis seedling development.
    Plant J., 2015. 83(3): p. 439-50
    [PMID:26047210]
  31. Smykowski A,Zimmermann P,Zentgraf U
    CORRECTION. G-Box Binding Factor1 Reduces CATALASE2 Expression and Regulates the Onset of Leaf Senescence in Arabidopsis.
    Plant Physiol., 2016. 170(2): p. 1164-7
    [PMID:26826725]
  32. Smykowski A,Fischer SM,Zentgraf U
    Phosphorylation Affects DNA-Binding of the Senescence-Regulating bZIP Transcription Factor GBF1.
    Plants (Basel), 2015. 4(3): p. 691-709
    [PMID:27135347]
  33. Giri MK, et al.
    GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana.
    Plant J., 2017. 91(5): p. 802-815
    [PMID:28622438]
  34. Klimczak LJ, et al.
    Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1.
    Plant Cell, 1995. 7(1): p. 105-15
    [PMID:7696877]
  35. Terzaghi WB,Bertekap RL,Cashmore AR
    Intracellular localization of GBF proteins and blue light-induced import of GBF2 fusion proteins into the nucleus of cultured Arabidopsis and soybean cells.
    Plant J., 1997. 11(5): p. 967-82
    [PMID:9193069]