PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G36540.2
Common NameAP22.3, BEE2, BHLH58, EN80
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 302aa    MW: 34398.5 Da    PI: 7.0568
Description BR enhanced expression 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G36540.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH26.51.2e-08152198555
                  HHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   5 hnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  h+ +Er RR++i +++  L++++P +    +k   Ka +L + ++Y++sLq
  AT4G36540.2 152 HSLAERARREKISKKMKCLQDIVPGC----NKVTGKAGMLDEIINYVQSLQ 198
                  999**********************9....677*****************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474593.79E-16145215IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.30E-7145202No hitNo description
PROSITE profilePS5088814.634147197IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.107.1E-17149214IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000102.7E-6152198IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.6E-10153203IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006351Biological Processtranscription, DNA-templated
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 302 aa     Download sequence    Send to blast
MDLSVLDRLK WLQQQQMVSP EFLQILGSDG REELKRVESY LGNNNDELQS FRHFPEFGPD  60
YDTTDGCISR TSSFHMEPVK NNGHSRAITL QNKRKPEGKT EKREKKKIKA EDETEPSMKG  120
KSNMSNTETS SEIQKPDYIH VRARRGEATD RHSLAERARR EKISKKMKCL QDIVPGCNKV  180
TGKAGMLDEI INYVQSLQQQ VEFLSMKLSV INPELECHID DLSAKQAYFT GPPEGDSKQS  240
IMADFRSFPL HQQGSLDYSV INSDHTTSLG AKDHTSSSWE THSQCLYNSL RTDSVSNFFS  300
LK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.254030.0leaf
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1865168830.0
Genevisible246275_at0.0
Expression AtlasAT4G36540-
AtGenExpressAT4G36540-
ATTED-IIAT4G36540-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in stems and flowers. {ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtPositive regulator of brassinosteroid signaling. {ECO:0000269|PubMed:12454087}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G36540.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by flagellin, jasmonic acid (JA), brassinosteroid and cytokinin, and repressed by abscisic acid. Insensitive to gibberellic acid. {ECO:0000269|PubMed:12454087, ECO:0000269|PubMed:12679534}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDbrassinosteroid
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G63090, AT1G30330
IntActSearch Q93VJ4
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No visible phenotype. Redundant with BEE1 and BEE3. {ECO:0000269|PubMed:12454087}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G36540
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF3702310.0AF370231.1 Arabidopsis thaliana unknown protein (At4g36540) mRNA, complete cds.
GenBankAY0591220.0AY059122.1 Arabidopsis thaliana unknown protein (At4g36540) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_849508.10.0BR enhanced expression 2
SwissprotQ93VJ40.0BEE2_ARATH; Transcription factor BEE 2
TrEMBLA0A178URK00.0A0A178URK0_ARATH; BEE2
STRINGAT4G36540.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Friedrichsen DM, et al.
    Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth.
    Genetics, 2002. 162(3): p. 1445-56
    [PMID:12454087]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  6. Hannah MA,Heyer AG,Hincha DK
    A global survey of gene regulation during cold acclimation in Arabidopsis thaliana.
    PLoS Genet., 2005. 1(2): p. e26
    [PMID:16121258]
  7. Husbands A,Bell EM,Shuai B,Smith HM,Springer PS
    LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins.
    Nucleic Acids Res., 2007. 35(19): p. 6663-71
    [PMID:17913740]
  8. Huang D,Wu W,Abrams SR,Cutler AJ
    The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors.
    J. Exp. Bot., 2008. 59(11): p. 2991-3007
    [PMID:18552355]
  9. Hu J, et al.
    Transcriptional responses of Arabidopsis thaliana during wilt disease caused by the soil-borne phytopathogenic bacterium, Ralstonia solanacearum.
    PLoS ONE, 2008. 3(7): p. e2589
    [PMID:18596930]
  10. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  11. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  12. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  13. Cifuentes-Esquivel N, et al.
    The bHLH proteins BEE and BIM positively modulate the shade avoidance syndrome in Arabidopsis seedlings.
    Plant J., 2013. 75(6): p. 989-1002
    [PMID:23763263]
  14. Singh M,Gupta A,Singh D,Khurana JP,Laxmi A
    Arabidopsis RSS1 Mediates Cross-Talk Between Glucose and Light Signaling During Hypocotyl Elongation Growth.
    Sci Rep, 2017. 7(1): p. 16101
    [PMID:29170398]