PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G32880.1
Common NameATHB8, ATHB-8, HB8, HB-8, T16I18.90
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HD-ZIP
Protein Properties Length: 833aa    MW: 91996.2 Da    PI: 6.2358
Description homeobox gene 8
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G32880.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Homeobox58.89.2e-191573357
                 --SS--HHHHHHHHHHHHHSSS--HHHHHHHHHHC....TS-HHHHHHHHHHHHHHHHC CS
     Homeobox  3 kRttftkeqleeLeelFeknrypsaeereeLAkkl....gLterqVkvWFqNrRakekk 57
                 k  ++t+eq+e+Le+l++ +++ps  +r++L +++    +++ +q+kvWFqNrR +ek+
  AT4G32880.1 15 KYVRYTPEQVEALERLYNDCPKPSSMRRQQLIRECpilsNIEPKQIKVWFQNRRCREKQ 73
                 5679*****************************************************97 PP

2START1931.5e-601603682205
                  HHHHHHHHHHHHHHHC-TT-EEEEEXCCTTEEEEEEESSS.SCEEEEEEEECCSCHHHHHHHHHCCCGGCT-TT-SEEEEEEEECTT..EEEEEEEEX CS
        START   2 laeeaaqelvkkalaeepgWvkssesengdevlqkfeeskvdsgealrasgvvdmvlallveellddkeqWdetlakaetlevissg..galqlmvae 97 
                  +a e+++e+++ka+ ++  Wv+++ +++g++++ +++ s++++g a+ra+g+v  +++  v+e+l+dk++W +++++ ++++v+s++  g+l+l++++
  AT4G32880.1 160 IADETLTEFISKATGTAVEWVQMPGMKPGPDSIGIVAISHGCTGIAARACGLVGLDPT-RVAEILKDKPCWLRDCRSLDIVNVLSTAngGTLELIYMQ 256
                  68899*****************************************************.9999****99*****************999********* PP

                  XTTXX-SSX.EEEEEEEEEEE.TTS-EEEEEEEEE-TTS--....-TTSEE-EESSEEEEEEEECTCEEEEEEEE-EE--SSXXHHHHHHHHHHHHHH CS
        START  98 lqalsplvp.RdfvfvRyirqlgagdwvivdvSvdseqkppe...sssvvRaellpSgiliepksnghskvtwvehvdlkgrlphwllrslvksglae 191
                  l+a+++l+p Rdf+++Ry+  +++g++vi+++S++++q+ p+   s+++vRae lpSg+li+p+++g+s +++v+h+dl+ ++++++lrsl++s++  
  AT4G32880.1 257 LYAPTTLAPaRDFWMLRYTSVMEDGSLVICERSLNNTQNGPSmppSPHFVRAEILPSGYLIRPCEGGGSILHIVDHFDLEPWSVPEVLRSLYESSTLL 354
                  ****************************************99999***************************************************** PP

                  HHHHHHHHTXXXXX CS
        START 192 gaktwvatlqrqce 205
                  +++t++a+l+++++
  AT4G32880.1 355 AQRTTMAALRYLRQ 368
                  *********99876 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5007115.6941074IPR001356Homeobox domain
SMARTSM003891.7E-151278IPR001356Homeobox domain
SuperFamilySSF466891.8E-161478IPR009057Homeodomain-like
CDDcd000861.19E-161575No hitNo description
PfamPF000462.7E-161673IPR001356Homeobox domain
Gene3DG3DSA:1.10.10.603.5E-181773IPR009057Homeodomain-like
CDDcd146861.14E-667106No hitNo description
PROSITE profilePS5084826.637150378IPR002913START domain
CDDcd088752.34E-81154369No hitNo description
Gene3DG3DSA:3.30.530.206.2E-24158364IPR023393START-like domain
SuperFamilySSF559618.38E-37159370No hitNo description
SMARTSM002344.0E-42159369IPR002913START domain
PfamPF018524.8E-58160368IPR002913START domain
PfamPF086708.6E-47691832IPR013978MEKHLA
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0008284Biological Processpositive regulation of cell proliferation
GO:0009733Biological Processresponse to auxin
GO:0010067Biological Processprocambium histogenesis
GO:0010072Biological Processprimary shoot apical meristem specification
GO:0010089Biological Processxylem development
GO:0045597Biological Processpositive regulation of cell differentiation
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008289Molecular Functionlipid binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000026anatomyprimary root tip
PO:0000036anatomyleaf vascular system
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0003021anatomycentral root cap of primary root
PO:0005352anatomyxylem
PO:0005597anatomycambium
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009015anatomyportion of vascular tissue
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009052anatomyflower pedicel
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0020149anatomyquiescent center
PO:0025022anatomycollective leaf structure
PO:0025275anatomyprocambium
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 833 aa     Download sequence    Send to blast
MGGGSNNSHN MDNGKYVRYT PEQVEALERL YNDCPKPSSM RRQQLIRECP ILSNIEPKQI  60
KVWFQNRRCR EKQRKEASRL QAVNRKLTAM NKLLMEENDR LQKQVSHLVY ENSYFRQHPQ  120
NQGNLATTDT SCESVVTSGQ HHLTPQHQPR DASPAGLLSI ADETLTEFIS KATGTAVEWV  180
QMPGMKPGPD SIGIVAISHG CTGIAARACG LVGLDPTRVA EILKDKPCWL RDCRSLDIVN  240
VLSTANGGTL ELIYMQLYAP TTLAPARDFW MLRYTSVMED GSLVICERSL NNTQNGPSMP  300
PSPHFVRAEI LPSGYLIRPC EGGGSILHIV DHFDLEPWSV PEVLRSLYES STLLAQRTTM  360
AALRYLRQIS QEISQPNVTG WGRRPAALRA LSQRLSKGFN EAVNGFSDEG WSILESDGID  420
DVTLLVNSSP TKMMMTSSLP FANGYTSMPS AVLCAKASML LQNVPPSILL RFLREHRQEW  480
ADNSIDAYSA AAIKAGPCSL PIPRPGSFGG QVILPLAHTI ENEEFMEVIK LESLGHYQED  540
MMMPADIFLL QMCSGVDENA VESCAELIFA PIDASFSDDA PIIPSGFRII PLDSKSEGLS  600
PNRTLDLASA LDVGSRTAGD SCGSRGNSKS VMTIAFQLAF EMHMQENVAS MARQYVRSVI  660
ASVQRVALAL SPSSHQLSGL RPPPASPEAH TLARWISHSY RCYLGVDLLK PHGTDLLKSL  720
WHHPDAVMCC SLKALSPVFT FANQAGLDML ETTLVALQDI TLDKIFDNNN GKKTLSSEFP  780
QIMQQGFMCM DGGICMSSMG RAVTYEKAVG WKVLNDDEDP HCICFMFLNW SFI
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.316280.0bud| floral meristem| leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453517240.0
Genevisible253402_at0.0
Expression AtlasAT4G32880-
AtGenExpressAT4G32880-
ATTED-IIAT4G32880-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in the procambial cells and the developing vascular system of embryos, roots and shoots. Expressed during the early stages of revascularization after cutting experiment. {ECO:0000269|PubMed:12447534, ECO:0000269|PubMed:8575317}.
Functional Description ? help Back to Top
Source Description
TAIRmember of homeodomain-leucine zipper family, acting as a differentiation-promoting transcription factor of the vascular meristems.
UniProtProbable transcription factor involved in the regulation of vascular development. May promote differentiation of precambial and cambial cells. {ECO:0000269|PubMed:11402194, ECO:0000269|PubMed:15598805}.
Function -- GeneRIF ? help Back to Top
  1. Class III HD-Zip AND KANADI genes required for root development [ATHB8]
    [PMID: 15286295]
  2. Data show that the expression of 3 DOF genes DOF2.1 (At2g28510), DOF4.6 (At4g24060) and DOF5.3 (At5g60200) is activated at early vascular development stages overlapping onset of ATHB8 expression.
    [PMID: 20563990]
  3. Here we discuss putative mechanisms by which wide domains of MP expression could activate ATHB8 transcription in single cell files.
    [PMID: 20592815]
  4. Congruence between SHR and ATHB8 expression domains persists under conditions of manipulated vein patterning, suggesting that inception of expression of SHR and ATHB8 identifies transition to a preprocambial cell state that presages vein formation.
    [PMID: 21128301]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G32880.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By auxin. Repressed by miR165. {ECO:0000269|PubMed:17237362, ECO:0000269|PubMed:8575317}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G19850 (A), AT5G11260 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT4G37650(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDauxin
Interaction ? help Back to Top
Source Intact With
IntActSearch Q39123
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G32880
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ4412920.0AJ441292.1 Arabidopsis thaliana mRNA for homeodomain-leucine zipper protein 8 (hb-8 gene).
GenBankAY0996310.0AY099631.1 Arabidopsis thaliana HD-zip transcription factor (athb-8) (At4g32880) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_195014.10.0homeobox-leucine zipper protein ATHB-8
SwissprotQ391230.0ATHB8_ARATH; Homeobox-leucine zipper protein ATHB-8
TrEMBLA0A178UUJ80.0A0A178UUJ8_ARATH; HB-8
STRINGAT4G32880.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM20222678
Representative plantOGRP6511671
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Baima S, et al.
    The arabidopsis ATHB-8 HD-zip protein acts as a differentiation-promoting transcription factor of the vascular meristems.
    Plant Physiol., 2001. 126(2): p. 643-55
    [PMID:11402194]
  3. Ohashi-Ito K,Demura T,Fukuda H
    Promotion of transcript accumulation of novel Zinnia immature xylem-specific HD-Zip III homeobox genes by brassinosteroids.
    Plant Cell Physiol., 2002. 43(10): p. 1146-53
    [PMID:12407194]
  4. Kang J,Dengler N
    Cell cycling frequency and expression of the homeobox gene ATHB-8 during leaf vein development in Arabidopsis.
    Planta, 2002. 216(2): p. 212-9
    [PMID:12447534]
  5. Mattsson J,Ckurshumova W,Berleth T
    Auxin signaling in Arabidopsis leaf vascular development.
    Plant Physiol., 2003. 131(3): p. 1327-39
    [PMID:12644682]
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  7. Hawker NP,Bowman JL
    Roles for Class III HD-Zip and KANADI genes in Arabidopsis root development.
    Plant Physiol., 2004. 135(4): p. 2261-70
    [PMID:15286295]
  8. Prigge MJ, et al.
    Class III homeodomain-leucine zipper gene family members have overlapping, antagonistic, and distinct roles in Arabidopsis development.
    Plant Cell, 2005. 17(1): p. 61-76
    [PMID:15598805]
  9. Williams L,Grigg SP,Xie M,Christensen S,Fletcher JC
    Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHD-ZIP target genes.
    Development, 2005. 132(16): p. 3657-68
    [PMID:16033795]
  10. Lee JY, et al.
    Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(15): p. 6055-60
    [PMID:16581911]
  11. Prigge MJ,Clark SE
    Evolution of the class III HD-Zip gene family in land plants.
    Evol. Dev., 2006 Jul-Aug. 8(4): p. 350-61
    [PMID:16805899]
  12. Alonso-Peral MM, et al.
    The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis.
    Development, 2006. 133(19): p. 3755-66
    [PMID:16943276]
  13. Zhong R,Demura T,Ye ZH
    SND1, a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis.
    Plant Cell, 2006. 18(11): p. 3158-70
    [PMID:17114348]
  14. Zhou GK,Kubo M,Zhong R,Demura T,Ye ZH
    Overexpression of miR165 affects apical meristem formation, organ polarity establishment and vascular development in Arabidopsis.
    Plant Cell Physiol., 2007. 48(3): p. 391-404
    [PMID:17237362]
  15. Petricka JJ,Nelson TM
    Arabidopsis nucleolin affects plant development and patterning.
    Plant Physiol., 2007. 144(1): p. 173-86
    [PMID:17369435]
  16. Chandler JW,Cole M,Flier A,Grewe B,Werr W
    The AP2 transcription factors DORNROSCHEN and DORNROSCHEN-LIKE redundantly control Arabidopsis embryo patterning via interaction with PHAVOLUTA.
    Development, 2007. 134(9): p. 1653-62
    [PMID:17376809]
  17. Kim YS, et al.
    HD-ZIP III activity is modulated by competitive inhibitors via a feedback loop in Arabidopsis shoot apical meristem development.
    Plant Cell, 2008. 20(4): p. 920-33
    [PMID:18408069]
  18. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  19. Donner TJ,Sherr I,Scarpella E
    Regulation of preprocambial cell state acquisition by auxin signaling in Arabidopsis leaves.
    Development, 2009. 136(19): p. 3235-46
    [PMID:19710171]
  20. Roschzttardtz H,Conéjéro G,Curie C,Mari S
    Identification of the endodermal vacuole as the iron storage compartment in the Arabidopsis embryo.
    Plant Physiol., 2009. 151(3): p. 1329-38
    [PMID:19726572]
  21. Ji J, et al.
    WOX4 promotes procambial development.
    Plant Physiol., 2010. 152(3): p. 1346-56
    [PMID:20044450]
  22. Gardiner J,Sherr I,Scarpella E
    Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves.
    Int. J. Dev. Biol., 2010. 54(8-9): p. 1389-96
    [PMID:20563990]
  23. Donner TJ,Sherr I,Scarpella E
    Auxin signal transduction in Arabidopsis vein formation.
    Plant Signal Behav, 2010. 5(1): p. 70-2
    [PMID:20592815]
  24. Hirakawa Y,Kondo Y,Fukuda H
    TDIF peptide signaling regulates vascular stem cell proliferation via the WOX4 homeobox gene in Arabidopsis.
    Plant Cell, 2010. 22(8): p. 2618-29
    [PMID:20729381]
  25. Pullen M,Clark N,Zarinkamar F,Topping J,Lindsey K
    Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.
    PLoS ONE, 2010. 5(8): p. e12227
    [PMID:20808926]
  26. Gardiner J,Donner TJ,Scarpella E
    Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development.
    Dev. Dyn., 2011. 240(1): p. 261-70
    [PMID:21128301]
  27. Agusti J,Lichtenberger R,Schwarz M,Nehlin L,Greb T
    Characterization of transcriptome remodeling during cambium formation identifies MOL1 and RUL1 as opposing regulators of secondary growth.
    PLoS Genet., 2011. 7(2): p. e1001312
    [PMID:21379334]
  28. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  29. Heyndrickx KS,Vandepoele K
    Systematic identification of functional plant modules through the integration of complementary data sources.
    Plant Physiol., 2012. 159(3): p. 884-901
    [PMID:22589469]
  30. Ohashi-Ito K,Oguchi M,Kojima M,Sakakibara H,Fukuda H
    Auxin-associated initiation of vascular cell differentiation by LONESOME HIGHWAY.
    Development, 2013. 140(4): p. 765-9
    [PMID:23362345]
  31. Hategan L,Godza B,Kozma-Bognar L,Bishop GJ,Szekeres M
    Differential expression of the brassinosteroid receptor-encoding BRI1 gene in Arabidopsis.
    Planta, 2014. 239(5): p. 989-1001
    [PMID:24488524]
  32. Baima S, et al.
    Negative feedback regulation of auxin signaling by ATHB8/ACL5-BUD2 transcription module.
    Mol Plant, 2014. 7(6): p. 1006-1025
    [PMID:24777988]
  33. Floyd SK, et al.
    Origin of a novel regulatory module by duplication and degeneration of an ancient plant transcription factor.
    Mol. Phylogenet. Evol., 2014. 81: p. 159-73
    [PMID:25263420]
  34. Kakehi J, et al.
    Mutations in ribosomal proteins, RPL4 and RACK1, suppress the phenotype of a thermospermine-deficient mutant of Arabidopsis thaliana.
    PLoS ONE, 2015. 10(1): p. e0117309
    [PMID:25625317]
  35. Jia X, et al.
    Functional plasticity of miR165/166 in plant development revealed by small tandem target mimic.
    Plant Sci., 2015. 233: p. 11-21
    [PMID:25711809]
  36. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  37. Liu S, et al.
    Expression of wild-type PtrIAA14.1, a poplar Aux/IAA gene causes morphological changes in Arabidopsis.
    Front Plant Sci, 2015. 6: p. 388
    [PMID:26082787]
  38. Yamaguchi YL, et al.
    Root-Knot and Cyst Nematodes Activate Procambium-Associated Genes in Arabidopsis Roots.
    Front Plant Sci, 2017. 8: p. 1195
    [PMID:28747918]
  39. Yamazaki K, et al.
    Suppression of DELLA signaling induces procambial cell formation in culture.
    Plant J., 2018. 94(1): p. 48-59
    [PMID:29383774]
  40. S
    Expression patterns of novel genes encoding homeodomain leucine-zipper proteins in Arabidopsis thaliana.
    Plant Mol. Biol., 1994. 26(1): p. 145-54
    [PMID:7948864]
  41. Baima S, et al.
    The expression of the Athb-8 homeobox gene is restricted to provascular cells in Arabidopsis thaliana.
    Development, 1995. 121(12): p. 4171-82
    [PMID:8575317]
  42. Sessa G,Steindler C,Morelli G,Ruberti I
    The Arabidopsis Athb-8, -9 and -14 genes are members of a small gene family coding for highly related HD-ZIP proteins.
    Plant Mol. Biol., 1998. 38(4): p. 609-22
    [PMID:9747806]