PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G17880.1
Common NameBHLH4, EN37, MYC4, T6K21.60
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 589aa    MW: 64588.6 Da    PI: 4.6347
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G17880.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH406.8e-13416461454
                  HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
          HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                  +h e+Er+RR+++N++f  Lr ++P+       K++Ka+ L  A+ YI++L
  AT4G17880.1 416 NHVEAERQRREKLNQRFYSLRAVVPNV-----SKMDKASLLGDAISYISEL 461
                  799***********************6.....5****************99 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142154.1E-5563250IPR025610Transcription factor MYC/MYB N-terminal
PROSITE profilePS5088817.187412461IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.07E-14415466No hitNo description
SuperFamilySSF474591.44E-18415482IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000102.6E-10416461IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.1E-18416481IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003538.2E-17418467IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006952Biological Processdefense response
GO:0009718Biological Processanthocyanin-containing compound biosynthetic process
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043425Molecular FunctionbHLH transcription factor binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000003anatomywhole plant
PO:0000013anatomycauline leaf
PO:0000034anatomyvascular system
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 589 aa     Download sequence    Send to blast
MSPTNVQVTD YHLNQSKTDT TNLWSTDDDA SVMEAFIGGG SDHSSLFPPL PPPPLPQVNE  60
DNLQQRLQAL IEGANENWTY AVFWQSSHGF AGEDNNNNNT VLLGWGDGYY KGEEEKSRKK  120
KSNPASAAEQ EHRKRVIREL NSLISGGVGG GDEAGDEEVT DTEWFFLVSM TQSFVKGTGL  180
PGQAFSNSDT IWLSGSNALA GSSCERARQG QIYGLQTMVC VATENGVVEL GSSEIIHQSS  240
DLVDKVDTFF NFNNGGGEFG SWAFNLNPDQ GENDPGLWIS EPNGVDSGLV AAPVMNNGGN  300
DSTSNSDSQP ISKLCNGSSV ENPNPKVLKS CEMVNFKNGI ENGQEEDSSN KKRSPVSNNE  360
EGMLSFTSVL PCDSNHSDLE ASVAKEAESN RVVVEPEKKP RKRGRKPANG REEPLNHVEA  420
ERQRREKLNQ RFYSLRAVVP NVSKMDKASL LGDAISYISE LKSKLQKAES DKEELQKQID  480
VMNKEAGNAK SSVKDRKCLN QESSVLIEME VDVKIIGWDA MIRIQCSKRN HPGAKFMEAL  540
KELDLEVNHA SLSVVNDLMI QQATVKMGNQ FFTQDQLKVA LTEKVGECP
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4rru_A4e-95182461227Transcription factor MYC3
4ywc_A4e-95182461227Transcription factor MYC3
4ywc_B4e-95182461227Transcription factor MYC3
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1397405KKPRKRGRK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.283160.0flower| seed| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible254693_at0.0
Expression AtlasAT4G17880-
AtGenExpressAT4G17880-
ATTED-IIAT4G17880-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively at low levels. Preferentially expressed in vascular tissues. {ECO:0000269|PubMed:12509522, ECO:0000269|PubMed:21335373}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in jasmonic acid (JA) gene regulation. With MYC2 and MYC3, controls additively subsets of JA-dependent responses. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the G-box (5'-CACGTG-3') of promoters. Activates multiple TIFY/JAZ promoters. {ECO:0000269|PubMed:21321051, ECO:0000269|PubMed:21335373, ECO:0000269|PubMed:23943862}.
Function -- GeneRIF ? help Back to Top
  1. results suggest that MYC3 and MYC4 are JAZ-interacting transcription factors that regulate jasmonate responses
    [PMID: 21321051]
  2. MYC3 and MYC4, act additively with MYC2 in the activation of jasmonic acid responses.
    [PMID: 21335373]
  3. MYC2/MYC3/MYC4 are necessary for direct transcriptional activation of GS biosynthesis genes.
    [PMID: 23943862]
  4. regulator of glucosinolate biosynthesis
    [PMID: 25049362]
  5. The function of MYC2, MYC3, and MYC4 in seed development and seed storage protein accumulation
    [PMID: 27415132]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00086PBM26531826Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G17880.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By UV treatment. Not induced by jasmonic acid. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:21335373}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G17880, AT5G07690, AT5G07700, AT5G40350, AT5G46760, AT5G60890, AT5G61420, AT1G18570, AT1G32640, AT1G74080
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Minor effect on jasmonic acid response and no effect on glucosinolate biosynthesis. Myc2 and myc4 double mutant has an increased insensitivity to jasmonic acid. Myc2, myc3 and myc4 triple mutant has no jasmonate-related defense response, is devoid of glucosinolates and is extremely susceptible to generalist herbivores. {ECO:0000269|PubMed:21321051, ECO:0000269|PubMed:21335373, ECO:0000269|PubMed:23943862}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G17880
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2215070.0AK221507.1 Arabidopsis thaliana mRNA for putative transcription factor BHLH4, complete cds, clone: RAFL07-57-K04.
GenBankAL0218890.0AL021889.2 Arabidopsis thaliana DNA chromosome 4, BAC clone T6K21 (ESSA project).
GenBankAL1615470.0AL161547.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 47.
GenBankCP0026870.0CP002687.1 Arabidopsis thaliana chromosome 4 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_193522.10.0Basic helix-loop-helix (bHLH) DNA-binding family protein
SwissprotO496870.0MYC4_ARATH; Transcription factor MYC4
TrEMBLA0A178URZ00.0A0A178URZ0_ARATH; MYC4
STRINGAT4G17880.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM12652896
Representative plantOGRP37331224
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Abe H, et al.
    Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell, 2003. 15(1): p. 63-78
    [PMID:12509522]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Lorenzo O,Chico JM,S
    JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis.
    Plant Cell, 2004. 16(7): p. 1938-50
    [PMID:15208388]
  7. Nagata T,Yamada H,Du Z,Todoriki S,Kikuchi S
    Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis.
    J. Agric. Food Chem., 2005. 53(4): p. 1022-30
    [PMID:15713015]
  8. Devoto A, et al.
    Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
    Plant Mol. Biol., 2005. 58(4): p. 497-513
    [PMID:16021335]
  9. Hanada K, et al.
    Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.
    Mol. Biol. Evol., 2011. 28(1): p. 377-82
    [PMID:20736450]
  10. Niu Y,Figueroa P,Browse J
    Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis.
    J. Exp. Bot., 2011. 62(6): p. 2143-54
    [PMID:21321051]
  11. Fern
    The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses.
    Plant Cell, 2011. 23(2): p. 701-15
    [PMID:21335373]
  12. Tominaga-Wada R,Iwata M,Nukumizu Y,Wada T
    Analysis of IIId, IIIe and IVa group basic-helix-loop-helix proteins expressed in Arabidopsis root epidermis.
    Plant Sci., 2011. 181(4): p. 471-8
    [PMID:21889054]
  13. Figueroa P,Browse J
    The Arabidopsis JAZ2 promoter contains a G-Box and thymidine-rich module that are necessary and sufficient for jasmonate-dependent activation by MYC transcription factors and repression by JAZ proteins.
    Plant Cell Physiol., 2012. 53(2): p. 330-43
    [PMID:22173100]

  14. MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis.
    Plant Physiol., 2012. 160(1): p. 541-55
    [PMID:22822211]
  15. Kazan K,Manners JM
    MYC2: the master in action.
    Mol Plant, 2013. 6(3): p. 686-703
    [PMID:23142764]
  16. Moreno JE, et al.
    Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10.
    Plant Physiol., 2013. 162(2): p. 1006-17
    [PMID:23632853]
  17. Schweizer F, et al.
    Arabidopsis basic helix-loop-helix transcription factors MYC2, MYC3, and MYC4 regulate glucosinolate biosynthesis, insect performance, and feeding behavior.
    Plant Cell, 2013. 25(8): p. 3117-32
    [PMID:23943862]
  18. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  19. Song S, et al.
    Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis.
    Plant Cell, 2014. 26(1): p. 263-79
    [PMID:24399301]
  20. Chico JM, et al.
    Repression of Jasmonate-Dependent Defenses by Shade Involves Differential Regulation of Protein Stability of MYC Transcription Factors and Their JAZ Repressors in Arabidopsis.
    Plant Cell, 2014. 26(5): p. 1967-1980
    [PMID:24824488]
  21. Frerigmann H,Berger B,Gigolashvili T
    bHLH05 is an interaction partner of MYB51 and a novel regulator of glucosinolate biosynthesis in Arabidopsis.
    Plant Physiol., 2014. 166(1): p. 349-69
    [PMID:25049362]
  22. Qi T,Huang H,Song S,Xie D
    Regulation of Jasmonate-Mediated Stamen Development and Seed Production by a bHLH-MYB Complex in Arabidopsis.
    Plant Cell, 2015. 27(6): p. 1620-33
    [PMID:26002869]
  23. Gasperini D, et al.
    Multilayered Organization of Jasmonate Signalling in the Regulation of Root Growth.
    PLoS Genet., 2015. 11(6): p. e1005300
    [PMID:26070206]
  24. Qi T, et al.
    Regulation of Jasmonate-Induced Leaf Senescence by Antagonism between bHLH Subgroup IIIe and IIId Factors in Arabidopsis.
    Plant Cell, 2015. 27(6): p. 1634-49
    [PMID:26071420]
  25. Pauwels L, et al.
    The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.
    Plant Physiol., 2015. 169(2): p. 1405-17
    [PMID:26320228]
  26. de Torres Zabala M, et al.
    Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection.
    New Phytol., 2016. 209(3): p. 1120-34
    [PMID:26428397]
  27. Yu J, et al.
    JAZ7 negatively regulates dark-induced leaf senescence in Arabidopsis.
    J. Exp. Bot., 2016. 67(3): p. 751-62
    [PMID:26547795]
  28. Chen X,Huang H,Qi T,Liu B,Song S
    New perspective of the bHLH-MYB complex in jasmonate-regulated plant fertility in arabidopsis.
    Plant Signal Behav, 2016. 11(2): p. e1135280
    [PMID:26829586]
  29. Schmiesing A,Emonet A,Gouhier-Darimont C,Reymond P
    Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract.
    Plant Physiol., 2016. 170(4): p. 2432-43
    [PMID:26884488]
  30. Thatcher LF, et al.
    Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum.
    J. Exp. Bot., 2016. 67(8): p. 2367-86
    [PMID:26896849]
  31. Gao C, et al.
    MYC2, MYC3, and MYC4 function redundantly in seed storage protein accumulation in Arabidopsis.
    Plant Physiol. Biochem., 2016. 108: p. 63-70
    [PMID:27415132]
  32. Gimenez-Ibanez S, et al.
    JAZ2 controls stomata dynamics during bacterial invasion.
    New Phytol., 2017. 213(3): p. 1378-1392
    [PMID:28005270]
  33. Lian TF,Xu YP,Li LF,Su XD
    Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the Mechanism of Enhanced Interaction with DNA.
    Cell Rep, 2017. 19(7): p. 1334-1342
    [PMID:28514654]
  34. Wang H, et al.
    The bHLH Transcription Factors MYC2, MYC3, and MYC4 Are Required for Jasmonate-Mediated Inhibition of Flowering in Arabidopsis.
    Mol Plant, 2017. 10(11): p. 1461-1464
    [PMID:28827172]
  35. Song S, et al.
    MYC5 is Involved in Jasmonate-Regulated Plant Growth, Leaf Senescence and Defense Responses.
    Plant Cell Physiol., 2017. 58(10): p. 1752-1763
    [PMID:29017003]
  36. Li B, et al.
    Network-Guided Discovery of Extensive Epistasis between Transcription Factors Involved in Aliphatic Glucosinolate Biosynthesis.
    Plant Cell, 2018. 30(1): p. 178-195
    [PMID:29317470]
  37. Han X, et al.
    Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons.
    Plant Physiol., 2018. 176(4): p. 2871-2885
    [PMID:29496884]