PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G01250.1
Common NameA_IG002N01.6, AtWRKY22, F2N1.6, WRKY22
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 298aa    MW: 32255.6 Da    PI: 7.566
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G01250.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY98.15.6e-31128186259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  +D ++WrKYGqK++kgs++pr+YYrC+++ gC ++k+ver+++dpk++++tY+ eHnh+
  AT4G01250.1 128 SDVWAWRKYGQKPIKGSPYPRGYYRCSTSkGCLARKQVERNRSDPKMFIVTYTAEHNHP 186
                  69************************9988****************************8 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.801.8E-30117188IPR003657WRKY domain
PROSITE profilePS5081136.77122188IPR003657WRKY domain
SuperFamilySSF1182903.92E-26124188IPR003657WRKY domain
SMARTSM007748.4E-36127187IPR003657WRKY domain
PfamPF031067.0E-26129186IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0006952Biological Processdefense response
GO:0010150Biological Processleaf senescence
GO:0010200Biological Processresponse to chitin
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 298 aa     Download sequence    Send to blast
MADDWDLHAV VRGCSAVSSS ATTTVYSPGV SSHTNPIFTV GRQSNAVSFG EIRDLYTPFT  60
QESVVSSFSC INYPEEPRKP QNQKRPLSLS ASSGSVTSKP SGSNTSRSKR RKIQHKKVCH  120
VAAEALNSDV WAWRKYGQKP IKGSPYPRGY YRCSTSKGCL ARKQVERNRS DPKMFIVTYT  180
AEHNHPAPTH RNSLAGSTRQ KPSDQQTSKS PTTTIATYSS SPVTSADEFV LPVEDHLAVG  240
DLDGEEDLLS LSDTVVSDDF FDGLEEFAAG DSFSGNSAPA SFDLSWVVNS AATTTGGI
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj2_A3e-181261881677Probable WRKY transcription factor 4
2lex_A3e-181261881677Probable WRKY transcription factor 4
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.38030.0bud| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible255568_at0.0
Expression AtlasAT4G01250-
AtGenExpressAT4G01250-
ATTED-IIAT4G01250-
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group II-e
UniProtTranscription factor involved in the expression of defense genes in innate immune response of plants. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Activates WRKY 29, SIRK and its own promoters. {ECO:0000269|PubMed:11875555}.
Function -- GeneRIF ? help Back to Top
  1. AtWRKY22 participates in the dark-induced senescence signal transduction pathway
    [PMID: 21359674]
  2. Data indicate that WRKY22 T-DNA insertion mutants wrky22-1 and wrky22-2 had lower disease resistance and lower induction of innate immunity markers, such as FLG22-INDUCED RECEPTOR-LIKE KINASE1 (FRK1) and WRKY53, after submergence.
    [PMID: 23897923]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00422DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G01250.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced after flagellin treatment. {ECO:0000269|PubMed:11875555}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G19190(A), AT4G23550(A)
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G01250
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0794040.0AY079404.1 Arabidopsis thaliana putative DNA-binding protein (At4g01250) mRNA, complete cds.
GenBankAY0459090.0AY045909.1 Arabidopsis thaliana putative DNA-binding protein (At4g01250) mRNA, complete cds.
GenBankAF4423920.0AF442392.1 Arabidopsis thaliana WRKY transcription factor 22 (WRKY22) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_192034.10.0WRKY transcription factor 22
SwissprotO046090.0WRK22_ARATH; WRKY transcription factor 22
TrEMBLD7M4X40.0D7M4X4_ARALL; WRKY transcription factor 22
STRINGAT4G01250.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP1417875
MalvidsOGEM78102741
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Asai T, et al.
    MAP kinase signalling cascade in Arabidopsis innate immunity.
    Nature, 2002. 415(6875): p. 977-83
    [PMID:11875555]
  4. Folta KM,Pontin MA,Karlin-Neumann G,Bottini R,Spalding EP
    Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light.
    Plant J., 2003. 36(2): p. 203-14
    [PMID:14535885]
  5. Dal Bosco C, et al.
    Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana.
    J. Biol. Chem., 2004. 279(2): p. 1060-9
    [PMID:14576160]
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  7. Hoth S, et al.
    Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana.
    FEBS Lett., 2003. 554(3): p. 373-80
    [PMID:14623097]
  8. Zipfel C, et al.
    Bacterial disease resistance in Arabidopsis through flagellin perception.
    Nature, 2004. 428(6984): p. 764-7
    [PMID:15085136]
  9. Navarro L, et al.
    The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis.
    Plant Physiol., 2004. 135(2): p. 1113-28
    [PMID:15181213]
  10. Hampton CR, et al.
    Cesium toxicity in Arabidopsis.
    Plant Physiol., 2004. 136(3): p. 3824-37
    [PMID:15489280]
  11. Monte E, et al.
    The phytochrome-interacting transcription factor, PIF3, acts early, selectively, and positively in light-induced chloroplast development.
    Proc. Natl. Acad. Sci. U.S.A., 2004. 101(46): p. 16091-8
    [PMID:15505214]
  12. Miao Y,Laun T,Zimmermann P,Zentgraf U
    Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis.
    Plant Mol. Biol., 2004. 55(6): p. 853-67
    [PMID:15604721]
  13. Xie Z, et al.
    Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells.
    Plant Physiol., 2005. 137(1): p. 176-89
    [PMID:15618416]
  14. Lee D,Polisensky DH,Braam J
    Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes.
    New Phytol., 2005. 165(2): p. 429-44
    [PMID:15720654]
  15. Lee BH,Henderson DA,Zhu JK
    The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell, 2005. 17(11): p. 3155-75
    [PMID:16214899]
  16. Phelps-Durr TL,Thomas J,Vahab P,Timmermans MC
    Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis.
    Plant Cell, 2005. 17(11): p. 2886-98
    [PMID:16243907]
  17. Suzuki N, et al.
    Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c.
    Plant Physiol., 2005. 139(3): p. 1313-22
    [PMID:16244138]
  18. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
    [PMID:16553893]
  19. Thilmony R,Underwood W,He SY
    Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.
    Plant J., 2006. 46(1): p. 34-53
    [PMID:16553894]
  20. Tosti N, et al.
    Gene expression profiles of O3-treated Arabidopsis plants.
    Plant Cell Environ., 2006. 29(9): p. 1686-702
    [PMID:16913859]
  21. M
    The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin.
    Plant J., 2006. 48(4): p. 485-98
    [PMID:17059410]
  22. Serrano M, et al.
    Chemical interference of pathogen-associated molecular pattern-triggered immune responses in Arabidopsis reveals a potential role for fatty-acid synthase type II complex-derived lipid signals.
    J. Biol. Chem., 2007. 282(9): p. 6803-11
    [PMID:17166839]
  23. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  24. Chawade A,Br
    Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors.
    BMC Genomics, 2007. 8: p. 304
    [PMID:17764576]
  25. Xu L, et al.
    Di- and tri- but not monomethylation on histone H3 lysine 36 marks active transcription of genes involved in flowering time regulation and other processes in Arabidopsis thaliana.
    Mol. Cell. Biol., 2008. 28(4): p. 1348-60
    [PMID:18070919]
  26. Wan J, et al.
    A LysM receptor-like kinase plays a critical role in chitin signaling and fungal resistance in Arabidopsis.
    Plant Cell, 2008. 20(2): p. 471-81
    [PMID:18263776]
  27. Yamaguchi Y,Huffaker A,Bryan AC,Tax FE,Ryan CA
    PEPR2 is a second receptor for the Pep1 and Pep2 peptides and contributes to defense responses in Arabidopsis.
    Plant Cell, 2010. 22(2): p. 508-22
    [PMID:20179141]
  28. Wan J,Zhang S,Stacey G
    Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin.
    Mol. Plant Pathol., 2004. 5(2): p. 125-35
    [PMID:20565589]
  29. Zhou X,Jiang Y,Yu D
    WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis.
    Mol. Cells, 2011. 31(4): p. 303-13
    [PMID:21359674]
  30. Schikora A, et al.
    N-acyl-homoserine lactone confers resistance toward biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6.
    Plant Physiol., 2011. 157(3): p. 1407-18
    [PMID:21940998]
  31. Gonz
    The conjugated auxin indole-3-acetic acid-aspartic acid promotes plant disease development.
    Plant Cell, 2012. 24(2): p. 762-77
    [PMID:22374398]
  32. Göhre V,Jones AM,Sklená? J,Robatzek S,Weber AP
    Molecular crosstalk between PAMP-triggered immunity and photosynthesis.
    Mol. Plant Microbe Interact., 2012. 25(8): p. 1083-92
    [PMID:22550958]
  33. Hsu FC, et al.
    Submergence confers immunity mediated by the WRKY22 transcription factor in Arabidopsis.
    Plant Cell, 2013. 25(7): p. 2699-713
    [PMID:23897923]
  34. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]