PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G00480.1
Common NameATMYC1, BHLH12, EN58, F6N23.22, myc1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 526aa    MW: 59987.8 Da    PI: 6.703
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G00480.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH18.53.6e-063463831355
                  HHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS
          HLH  13 RdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 
                  R++ N++f+ Lr+++P+ +       ++K +iL  +++Y+++L+
  AT4G00480.1 346 RRKENEKFSVLRTMVPTvN------EVDKESILNNTIKYLQELE 383
                  6777*************65......8***************995 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142152.1E-4923216IPR025610Transcription factor MYC/MYB N-terminal
PROSITE profilePS5088811.274333382IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.9E-8339388IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000832.19E-5346387No hitNo description
Gene3DG3DSA:4.10.280.105.7E-9346393IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474599.55E-9346397IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0048629Biological Processtrichome patterning
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000256anatomyroot hair cell
PO:0000262anatomytrichoblast
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 526 aa     Download sequence    Send to blast
MSLTMADGVE AAAGRSKRQN SLLRKQLALA VRSVQWSYAI FWSSSLTQPG VLEWGEGCYN  60
GDMKKRKKSY ESHYKYGLQK SKELRKLYLS MLEGDSGTTV STTHDNLNDD DDNCHSTSMM  120
LSPDDLSDEE WYYLVSMSYV FSPSQCLPGR ASATGETIWL CNAQYAENKL FSRSLLARSA  180
SIQTVVCFPY LGGVIELGVT ELISEDHNLL RNIKSCLMEI SAHQDNDDEK KMEIKISEEK  240
HQLPLGISDE DLHYKRTIST VLNYSADRSG KNDKNIRHRQ PNIVTSEPGS SFLRWKQCEQ  300
QVSGFVQKKK SQNVLRKILH DVPLMHTKRM FPSQNSGLNQ DDPSDRRKEN EKFSVLRTMV  360
PTVNEVDKES ILNNTIKYLQ ELEARVEELE SCMGSVNFVE RQRKTTENLN DSVLIEETSG  420
NYDDSTKIDD NSGETEQVTV FRDKTHLRVK LKETEVVIEV RCSYRDYIVA DIMETLSNLH  480
MDAFSVRSHT LNKFLTLNLK AKFRGAAVAS VGMIKRELRR VIGDLF
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4rqw_A2e-12232179192Transcription factor MYC3
4rqw_B2e-12232179192Transcription factor MYC3
4rs9_A2e-12232179192Transcription factor MYC3
4yz6_A2e-12232179192Transcription factor MYC3
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
16367KKRKK
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible255675_at0.0
Expression AtlasAT4G00480-
AtGenExpressAT4G00480-
ATTED-IIAT4G00480-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Mostly expressed in developing seeds. Also detected in stems and leaves. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:8980509}.
Functional Description ? help Back to Top
Source Description
TAIRMYC-related protein with a basic helix-loop-helix motif at the C-terminus and a region similar to the maize B/R family at the N-terminus
UniProtTrancsription activator, when associated with MYB75/PAP1 or MYB90/PAP2. {ECO:0000269|PubMed:15361138}.
Function -- GeneRIF ? help Back to Top
  1. Natural allelic variation defines a role for ATMYC1: trichome cell fate determination
    [PMID: 21695236]
  2. AtMYC1 is an important regulator of trichome and root hair initiation
    [PMID: 22334670]
  3. AtMYC1 protein is predominantly localised in the cytoplasm and can relocate GL1 from the nucleus into the cytoplasm.
    [PMID: 23900543]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G00480.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By cold, UV, flagellin, jasmonic acid (JA), and salicylic acid (SA) treatments. {ECO:0000269|PubMed:12679534}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction -- BIND ? help Back to Top
Source Intact With Description
BINDAT1G56650MYC1 (AtBHLH012) interacts with PAP1.
BINDAT1G66370MYC1 (AtBHLH012) interacts with MYB113.
BINDAT1G66380MYC1 (AtBHLH012) interacts with MYB114.
BINDAT1G66390MYC1 (AtBHLH012) interacts with PAP2.
BINDAT4G38620MYC1 (AtBHLH012) interacts with MYB4.
BINDAT5G14750MYC1 (AtBHLH012) interacts with WER.
BINDAT5G35550MYC1 (AtBHLH012) interacts with TT2.
BINDAT5G40330MYC1 (AtBHLH012) interacts with AtMYB23.
BINDAT5G52600MYC1 (AtBHLH012) interacts with MYB82.
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G38620, AT5G14750, AT5G35550, AT5G40330, AT5G53200, AT1G56650, AT1G66370, AT1G66380, AT1G66390
IntActSearch Q8W2F1
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G00480
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB4936640.0AB493664.1 Arabidopsis thaliana At4g00480 mRNA for hypothetical protein, partial cds, clone: RAAt4g00480.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191957.20.0basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ8W2F10.0BH012_ARATH; Transcription factor MYC1
TrEMBLC0SVG10.0C0SVG1_ARATH; MYC1 (Fragment)
STRINGAT4G00480.20.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  3. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  4. Ramsay NA,Walker AR,Mooney M,Gray JC
    Two basic-helix-loop-helix genes (MYC-146 and GL3) from Arabidopsis can activate anthocyanin biosynthesis in a white-flowered Matthiola incana mutant.
    Plant Mol. Biol., 2003. 52(3): p. 679-88
    [PMID:12956536]
  5. Folta KM,Pontin MA,Karlin-Neumann G,Bottini R,Spalding EP
    Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light.
    Plant J., 2003. 36(2): p. 203-14
    [PMID:14535885]
  6. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  7. Baudry A, et al.
    TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana.
    Plant J., 2004. 39(3): p. 366-80
    [PMID:15255866]
  8. Zimmermann IM,Heim MA,Weisshaar B,Uhrig JF
    Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins.
    Plant J., 2004. 40(1): p. 22-34
    [PMID:15361138]
  9. Morohashi K,Grotewold E
    A systems approach reveals regulatory circuitry for Arabidopsis trichome initiation by the GL3 and GL1 selectors.
    PLoS Genet., 2009. 5(2): p. e1000396
    [PMID:19247443]
  10. Hichri I, et al.
    The basic helix-loop-helix transcription factor MYC1 is involved in the regulation of the flavonoid biosynthesis pathway in grapevine.
    Mol Plant, 2010. 3(3): p. 509-23
    [PMID:20118183]
  11. Symonds VV,Hatlestad G,Lloyd AM
    Natural allelic variation defines a role for ATMYC1: trichome cell fate determination.
    PLoS Genet., 2011. 7(6): p. e1002069
    [PMID:21695236]
  12. Bruex A, et al.
    A gene regulatory network for root epidermis cell differentiation in Arabidopsis.
    PLoS Genet., 2012. 8(1): p. e1002446
    [PMID:22253603]
  13. Zhao H, et al.
    A single amino acid substitution in IIIf subfamily of basic helix-loop-helix transcription factor AtMYC1 leads to trichome and root hair patterning defects by abolishing its interaction with partner proteins in Arabidopsis.
    J. Biol. Chem., 2012. 287(17): p. 14109-21
    [PMID:22334670]
  14. Pesch M,Schulthei
    Mutual control of intracellular localisation of the patterning proteins AtMYC1, GL1 and TRY/CPC in Arabidopsis.
    Development, 2013. 140(16): p. 3456-67
    [PMID:23900543]
  15. Urao T,Yamaguchi-Shinozaki K,Mitsukawa N,Shibata D,Shinozaki K
    Molecular cloning and characterization of a gene that encodes a MYC-related protein in Arabidopsis.
    Plant Mol. Biol., 1996. 32(3): p. 571-6
    [PMID:8980509]