PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G00050.1
Common NameBHLH16, EN108, F6N15.11, UNE10
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 399aa    MW: 43365.2 Da    PI: 7.5542
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G00050.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH49.29.8e-16218263555
                  HHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   5 hnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  hn+ Er+RRd+iN+++ +L++l+P++      K +Ka++L +++eY+k+Lq
  AT4G00050.1 218 HNQSERKRRDKINQRMKTLQKLVPNS-----SKTDKASMLDEVIEYLKQLQ 263
                  9************************8.....7******************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474591.22E-18211274IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088817.815213262IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000835.72E-17218267No hitNo description
PfamPF000102.6E-13218263IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.107.2E-18218272IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003537.4E-18219268IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009567Biological Processdouble fertilization forming a zygote and endosperm
GO:0005634Cellular Componentnucleus
GO:0009506Cellular Componentplasmodesma
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 399 aa     Download sequence    Send to blast
MSQCVPNCHI DDTPAAATTT VRSTTAADIP ILDYEVAELT WENGQLGLHG LGPPRVTASS  60
TKYSTGAGGT LESIVDQATR LPNPKPTDEL VPWFHHRSSR AAMAMDALVP CSNLVHEQQS  120
KPGGVGSTRV GSCSDGRTMG GGKRARVAPE WSGGGSQRLT MDTYDVGFTS TSMGSHDNTI  180
DDHDSVCHSR PQMEDEEEKK AGGKSSVSTK RSRAAAIHNQ SERKRRDKIN QRMKTLQKLV  240
PNSSKTDKAS MLDEVIEYLK QLQAQVSMMS RMNMPSMMLP MAMQQQQQLQ MSLMSNPMGL  300
GMGMGMPGLG LLDLNSMNRA AASAPNIHAN MMPNPFLPMN CPSWDASSND SRFQSPLIPD  360
PMSAFLACST QPTTMEAYSR MATLYQQMQQ QLPPPSNPK
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1221226ERKRRD
2222227RKRRDK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.447790.0silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible255694_at0.0
Expression AtlasAT4G00050-
AtGenExpressAT4G00050-
ATTED-IIAT4G00050-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in stems and flowers. {ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtRequired during the fertilization of ovules by pollen. {ECO:0000269|PubMed:15634699}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00034PBM25215497Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G00050.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By cold treatment. {ECO:0000269|PubMed:12679534}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G14920
IntActSearch Q8GZ38
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Plants exhibit unfertilized ovules but normal pollen tube attraction. {ECO:0000269|PubMed:15634699}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G00050
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK1172290.0AK117229.1 Arabidopsis thaliana At4g00050 mRNA for putative bHLH transcription factor (bHLH016), complete cds, clone: RAFL16-78-C08.
GenBankBT0259710.0BT025971.1 Arabidopsis thaliana At4g00050 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191916.30.0basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ8GZ380.0UNE10_ARATH; Transcription factor UNE10
TrEMBLA0A178V2U00.0A0A178V2U0_ARATH; UNE10
STRINGAT4G00050.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM39192451
Representative plantOGRP80031014
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
    [PMID:11910074]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Devlin PF,Yanovsky MJ,Kay SA
    A genomic analysis of the shade avoidance response in Arabidopsis.
    Plant Physiol., 2003. 133(4): p. 1617-29
    [PMID:14645734]
  7. Becher M,Talke IN,Krall L,Kr
    Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri.
    Plant J., 2004. 37(2): p. 251-68
    [PMID:14690509]
  8. Tepperman JM, et al.
    Expression profiling of phyB mutant demonstrates substantial contribution of other phytochromes to red-light-regulated gene expression during seedling de-etiolation.
    Plant J., 2004. 38(5): p. 725-39
    [PMID:15144375]
  9. Pagnussat GC, et al.
    Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis.
    Development, 2005. 132(3): p. 603-14
    [PMID:15634699]
  10. Leivar P, et al.
    The Arabidopsis phytochrome-interacting factor PIF7, together with PIF3 and PIF4, regulates responses to prolonged red light by modulating phyB levels.
    Plant Cell, 2008. 20(2): p. 337-52
    [PMID:18252845]
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  12. Jaspers P, et al.
    Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors.
    Plant J., 2009. 60(2): p. 268-79
    [PMID:19548978]
  13. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  14. Hanada K, et al.
    Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.
    Mol. Biol. Evol., 2011. 28(1): p. 377-82
    [PMID:20736450]
  15. Fernandez-Calvino L, et al.
    Arabidopsis plasmodesmal proteome.
    PLoS ONE, 2011. 6(4): p. e18880
    [PMID:21533090]
  16. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  17. Mar
    Large-scale identification of gibberellin-related transcription factors defines group VII ETHYLENE RESPONSE FACTORS as functional DELLA partners.
    Plant Physiol., 2014. 166(2): p. 1022-32
    [PMID:25118255]
  18. Qiu Y, et al.
    HEMERA Couples the Proteolysis and Transcriptional Activity of PHYTOCHROME INTERACTING FACTORs in Arabidopsis Photomorphogenesis.
    Plant Cell, 2015. 27(5): p. 1409-27
    [PMID:25944101]