PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G61630.1
Common NameCRF6, ERF067, F15G16.20
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 315aa    MW: 35573.3 Da    PI: 4.6884
Description cytokinin response factor 6
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G61630.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP259.48.5e-19103153155
          AP2   1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  ++y+GVr+++ +g+++AeIrdps+   r+r++lg+f taeeAa a+++a+  l+g
  AT3G61630.1 103 KKYRGVRQRP-WGKFAAEIRDPSS---RTRIWLGTFVTAEEAAIAYDRAAIHLKG 153
                  59********.**********965...4**********************99987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000182.26E-31103160No hitNo description
SuperFamilySSF541718.5E-21104161IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.102.6E-30104160IPR001471AP2/ERF domain
PfamPF008471.5E-11104153IPR001471AP2/ERF domain
PROSITE profilePS5103221.931104161IPR001471AP2/ERF domain
SMARTSM003807.5E-36104167IPR001471AP2/ERF domain
PRINTSPR003671.6E-10105116IPR001471AP2/ERF domain
PRINTSPR003671.6E-10127143IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009736Biological Processcytokinin-activated signaling pathway
GO:0009873Biological Processethylene-activated signaling pathway
GO:0042991Biological Processtranscription factor import into nucleus
GO:0048825Biological Processcotyledon development
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 315 aa     Download sequence    Send to blast
MERRTRRVKF TENRTVTNVA ATPSNGSPRL VRITVTDPFA TDSSSDDDDN NNVTVVPRVK  60
RYVKEIRFCQ GESSSSTAAR KGKHKEEESV VVEDDVSTSV KPKKYRGVRQ RPWGKFAAEI  120
RDPSSRTRIW LGTFVTAEEA AIAYDRAAIH LKGPKALTNF LTPPTPTPVI DLQTVSACDY  180
GRDSRQSLHS PTSVLRFNVN EETEHEIEAI ELSPERKSTV IKEEEESSAG LVFPDPYLLP  240
DLSLAGECFW DTEIAPDLLF LDEETKIQST LLPNTEVSKQ GENETEDFEF GLIDDFESSP  300
WDVDHFFDHH HHSFD
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2gcc_A2e-23101160262ATERF1
3gcc_A2e-23101160262ATERF1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.342580.0leaf| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453397430.0
Genevisible251282_at0.0
Expression AtlasAT3G61630-
AtGenExpressAT3G61630-
ATTED-IIAT3G61630-
Functional Description ? help Back to Top
Source Description
TAIRCRF6 encodes one of the six cytokinin response factors. CRF5 belongs to the AP2/ERF superfamily of the transcriptional factors. CRF proteins rapidly relocalize to the nucleus in response to cytokinin. Analysis of loos-of-function mutants revealed that the CRFs function redundantly to regulate the development of embryos, cotyledons and leaves.
UniProtComponent of the cytokinin signaling pathway involved in cotyledons, leaves, and embryos development. Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
Function -- GeneRIF ? help Back to Top
  1. CRF6 functions to regulate developmental senescence negatively and may have a similar role in response to stress.
    [PMID: 23539244]
  2. CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development via transcriptional regulation of PIN-FORMED auxin transporters.
    [PMID: 26541513]
  3. Data show that cytokinin response factor 6 (CRF6) functions during oxidative stress as a negative regulator to control this cytokinin-associated module of CRF6-dependent genes and establishes a novel connection between cytokinin and oxidative stress response.
    [PMID: 27550996]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G61630.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By cytokinins. {ECO:0000269|PubMed:16832061}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G61630, AT4G11140, AT4G23750, AT4G27950, AT4G31920, AT5G53290, AT1G22985, AT1G71130
IntActSearch Q9M374
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G61630
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAL1329590.0AL132959.1 Arabidopsis thaliana DNA chromosome 3, BAC clone F15G16.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191722.10.0cytokinin response factor 6
SwissprotQ9M3740.0CRF6_ARATH; Ethylene-responsive transcription factor CRF6
TrEMBLA0A178VFH20.0A0A178VFH2_ARATH; CRF6
STRINGAT3G61630.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM25782571
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Castelli V, et al.
    Whole genome sequence comparisons and "full-length" cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation.
    Genome Res., 2004. 14(3): p. 406-13
    [PMID:14993207]
  3. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  4. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
    [PMID:16553893]
  5. Rashotte AM, et al.
    A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(29): p. 11081-5
    [PMID:16832061]
  6. Culligan KM,Robertson CE,Foreman J,Doerner P,Britt AB
    ATR and ATM play both distinct and additive roles in response to ionizing radiation.
    Plant J., 2006. 48(6): p. 947-61
    [PMID:17227549]
  7. Ramel F, et al.
    Genome-wide interacting effects of sucrose and herbicide-mediated stress in Arabidopsis thaliana: novel insights into atrazine toxicity and sucrose-induced tolerance.
    BMC Genomics, 2007. 8: p. 450
    [PMID:18053238]
  8. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  9. Inz
    A subcellular localization compendium of hydrogen peroxide-induced proteins.
    Plant Cell Environ., 2012. 35(2): p. 308-20
    [PMID:21443605]
  10. Cutcliffe JW,Hellmann E,Heyl A,Rashotte AM
    CRFs form protein-protein interactions with each other and with members of the cytokinin signalling pathway in Arabidopsis via the CRF domain.
    J. Exp. Bot., 2011. 62(14): p. 4995-5002
    [PMID:21705390]
  11. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  12. Zwack PJ,Robinson BR,Risley MG,Rashotte AM
    Cytokinin response factor 6 negatively regulates leaf senescence and is induced in response to cytokinin and numerous abiotic stresses.
    Plant Cell Physiol., 2013. 54(6): p. 971-81
    [PMID:23539244]
  13. Zwack PJ,Rashotte AM
    Cytokinin inhibition of leaf senescence.
    Plant Signal Behav, 2013. 8(7): p. e24737
    [PMID:23656876]
  14. Šimášková M, et al.
    Cytokinin response factors regulate PIN-FORMED auxin transporters.
    Nat Commun, 2015. 6: p. 8717
    [PMID:26541513]
  15. Zwack PJ, et al.
    Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.
    Plant Physiol., 2016. 172(2): p. 1249-1258
    [PMID:27550996]