PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G56400.1
Common NameATWRKY70, T5P19.50, WRKY70
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 294aa    MW: 32935.6 Da    PI: 6.2319
Description WRKY DNA-binding protein 70
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G56400.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY84.11.3e-26120180259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                  +D ++WrKYGqKe+ +++fprsY+rCt++   gC+++k+v++ + +pk++ itY g+H+++
  AT3G56400.1 120 EDAFSWRKYGQKEILNAKFPRSYFRCTHKytqGCKATKQVQKVELEPKMFSITYIGNHTCN 180
                  8***************************99999**************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.804.5E-26106180IPR003657WRKY domain
PROSITE profilePS5081120.656114182IPR003657WRKY domain
SuperFamilySSF1182901.31E-23114181IPR003657WRKY domain
SMARTSM007741.2E-37119181IPR003657WRKY domain
PfamPF031062.3E-24120179IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009862Biological Processsystemic acquired resistance, salicylic acid mediated signaling pathway
GO:0009864Biological Processinduced systemic resistance, jasmonic acid mediated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0050832Biological Processdefense response to fungus
GO:1900056Biological Processnegative regulation of leaf senescence
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 294 aa     Download sequence    Send to blast
MDTNKAKKLK VMNQLVEGHD LTTQLQQLLS QPGSGLEDLV AKILVCFNNT ISVLDTFEPI  60
SSSSSLAAVE GSQNASCDND GKFEDSGDSR KRLGPVKGKR GCYKRKKRSE TCTIESTILE  120
DAFSWRKYGQ KEILNAKFPR SYFRCTHKYT QGCKATKQVQ KVELEPKMFS ITYIGNHTCN  180
TNAETPKSKT CDHHDEIFMD SEDHKSPSLS TSMKEEDNPH RHHGSSTEND LSLVWPEMVF  240
EEDYHHQASY VNGKTSTSID VLGSQDLMVF GGGGDFEFSE NEHFSIFSSC SNLS
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
189107RKRLGPVKGKRGCYKRKKR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.52420.0leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO145326170.0
Genevisible251705_at0.0
Expression AtlasAT3G56400-
AtGenExpressAT3G56400-
ATTED-IIAT3G56400-
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group III. Function as activator of SA-dependent defense genes and a repressor of JA-regulated genes. WRKY70-controlled suppression of JA-signaling is partly executed by NPR1.
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.
Function -- GeneRIF ? help Back to Top
  1. WRKY70 has a pivotal role in determining the balance between SA-dependent and JA-dependent defense pathways.
    [PMID: 16623907]
  2. To expand understanding on how transcriptional control coordinates leaf senescence, AtWRKY70, a gene encoding a WRKY transcription factor that functions as a negative regulator of developmental senescence was characterized.
    [PMID: 17310369]
  3. The inactivation of the WRKY70 gene in wrky70-1 mutant does not alter the responses of both jasmonic acid (JA) and salicylic acid (SA), and that wrky70 mutation is unable to restore the coi1 mutant in JA responses.
    [PMID: 18713432]
  4. WRKY70 is required for snc2-1D-mediated resistance.
    [PMID: 20841424]
  5. WRKY54 and WRKY70 are negative regulators of leaf senescence.
    [PMID: 22268143]
  6. WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense.
    [PMID: 22325892]
  7. AtMYB44 modulates antagonistic interaction by activating SA-mediated defenses and repressing JA-mediated defenses through direct control of WRKY70
    [PMID: 23067202]
  8. AtMYB44 regulates defense responses by transcriptional activation of downstream gene, WRKY70.
    [PMID: 23603962]
  9. WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling.
    [PMID: 23815736]
  10. Data indicate that the nucleotides CGAC are essential for WRKY70-activated gene expression.
    [PMID: 24104863]
  11. A lower expression level of WRKY70 leads to significantly higher MYC2 expression through salicylic acid (SA)-jasmonic acid (JA) cross-talk.
    [PMID: 25339349]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00408DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G56400.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT2G24570 (R), AT4G31550 (R)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G19670(R), AT1G75040(A), AT2G14560(A), AT2G14610(A), AT2G18660(A), AT3G57260(A), AT4G24240(R), AT5G44420(R)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDjasmonic acid, salicylic acid
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G24110
IntActSearch Q9LY00
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G56400
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY1425660.0AY142566.1 Arabidopsis thaliana putative DNA-binding protein (At3g56400) mRNA, complete cds.
GenBankAY0399330.0AY039933.1 Arabidopsis thaliana putative DNA-binding protein (At3g56400) mRNA, complete cds.
GenBankAF4211570.0AF421157.1 Arabidopsis thaliana WRKY transcription factor 70 (WRKY70) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191199.10.0WRKY transcription factor 70
SwissprotQ9LY000.0WRK70_ARATH; Probable WRKY transcription factor 70
TrEMBLD7LVF50.0D7LVF5_ARALL; WRKY DNA-binding protein 70
STRINGAT3G56400.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP1417875
MalvidsOGEM40612757
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  4. Li J,Brader G,Palva ET
    The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense.
    Plant Cell, 2004. 16(2): p. 319-31
    [PMID:14742872]
  5. Dong X
    NPR1, all things considered.
    Curr. Opin. Plant Biol., 2004. 7(5): p. 547-52
    [PMID:15337097]
  6. Lorenzo O,Solano R
    Molecular players regulating the jasmonate signalling network.
    Curr. Opin. Plant Biol., 2005. 8(5): p. 532-40
    [PMID:16039901]
  7. McGrath KC, et al.
    Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression.
    Plant Physiol., 2005. 139(2): p. 949-59
    [PMID:16183832]
  8. Li J,Brader G,Kariola T,Palva ET
    WRKY70 modulates the selection of signaling pathways in plant defense.
    Plant J., 2006. 46(3): p. 477-91
    [PMID:16623907]
  9. Brodersen P, et al.
    Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4.
    Plant J., 2006. 47(4): p. 532-46
    [PMID:16813576]
  10. AbuQamar S, et al.
    Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection.
    Plant J., 2006. 48(1): p. 28-44
    [PMID:16925600]
  11. Kim KC,Fan B,Chen Z
    Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae.
    Plant Physiol., 2006. 142(3): p. 1180-92
    [PMID:16963526]
  12. Wang D,Amornsiripanitch N,Dong X
    A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants.
    PLoS Pathog., 2006. 2(11): p. e123
    [PMID:17096590]
  13. Journot-Catalino N,Somssich IE,Roby D,Kroj T
    The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana.
    Plant Cell, 2006. 18(11): p. 3289-302
    [PMID:17114354]
  14. Ciftci-Yilmaz S, et al.
    The EAR-motif of the Cys2/His2-type zinc finger protein Zat7 plays a key role in the defense response of Arabidopsis to salinity stress.
    J. Biol. Chem., 2007. 282(12): p. 9260-8
    [PMID:17259181]
  15. Ulker B,Shahid Mukhtar M,Somssich IE
    The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways.
    Planta, 2007. 226(1): p. 125-37
    [PMID:17310369]
  16. Knoth C,Ringler J,Dangl JL,Eulgem T
    Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica.
    Mol. Plant Microbe Interact., 2007. 20(2): p. 120-8
    [PMID:17313163]
  17. Miao Y,Zentgraf U
    The antagonist function of Arabidopsis WRKY53 and ESR/ESP in leaf senescence is modulated by the jasmonic and salicylic acid equilibrium.
    Plant Cell, 2007. 19(3): p. 819-30
    [PMID:17369373]
  18. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  19. Alvarez-Venegas R,Abdallat AA,Guo M,Alfano JR,Avramova Z
    Epigenetic control of a transcription factor at the cross section of two antagonistic pathways.
    Epigenetics, 2007 Apr-Jun. 2(2): p. 106-13
    [PMID:17965588]
  20. Meier S, et al.
    Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides.
    BMC Plant Biol., 2008. 8: p. 24
    [PMID:18307823]
  21. Koornneef A,Pieterse CM
    Cross talk in defense signaling.
    Plant Physiol., 2008. 146(3): p. 839-44
    [PMID:18316638]
  22. Knoth C,Eulgem T
    The oomycete response gene LURP1 is required for defense against Hyaloperonospora parasitica in Arabidopsis thaliana.
    Plant J., 2008. 55(1): p. 53-64
    [PMID:18346188]
  23. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  24. Kim CY,Bove J,Assmann SM
    Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.
    New Phytol., 2008. 180(1): p. 57-70
    [PMID:18705666]
  25. Ren CM, et al.
    Transcription factor WRKY70 displays important but no indispensable roles in jasmonate and salicylic acid signaling.
    J Integr Plant Biol, 2008. 50(5): p. 630-7
    [PMID:18713432]
  26. Ishikawa K, et al.
    AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1-dependent salicylic acid signaling.
    Plant Physiol., 2010. 152(4): p. 2000-12
    [PMID:20181750]
  27. Zhang Y, et al.
    Arabidopsis snc2-1D activates receptor-like protein-mediated immunity transduced through WRKY70.
    Plant Cell, 2010. 22(9): p. 3153-63
    [PMID:20841424]
  28. Ndamukong I,Jones DR,Lapko H,Divecha N,Avramova Z
    Phosphatidylinositol 5-phosphate links dehydration stress to the activity of ARABIDOPSIS TRITHORAX-LIKE factor ATX1.
    PLoS ONE, 2010. 5(10): p. e13396
    [PMID:20967218]
  29. Shang J, et al.
    A broad-spectrum, efficient and nontransgenic approach to control plant viruses by application of salicylic acid and jasmonic acid.
    Planta, 2011. 233(2): p. 299-308
    [PMID:21046144]
  30. Zhou X,Jiang Y,Yu D
    WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis.
    Mol. Cells, 2011. 31(4): p. 303-13
    [PMID:21359674]
  31. Sharon M,Freeman S,Sneh B
    Assessment of resistance pathways induced in Arabidopsis thaliana by hypovirulent Rhizoctonia spp. isolates.
    Phytopathology, 2011. 101(7): p. 828-38
    [PMID:21385012]
  32. Atamian HS,Eulgem T,Kaloshian I
    SlWRKY70 is required for Mi-1-mediated resistance to aphids and nematodes in tomato.
    Planta, 2012. 235(2): p. 299-309
    [PMID:21898085]
  33. von Saint Paul V, et al.
    The Arabidopsis glucosyltransferase UGT76B1 conjugates isoleucic acid and modulates plant defense and senescence.
    Plant Cell, 2011. 23(11): p. 4124-45
    [PMID:22080599]
  34. Besseau S,Li J,Palva ET
    WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana.
    J. Exp. Bot., 2012. 63(7): p. 2667-79
    [PMID:22268143]
  35. Moreau M, et al.
    EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora.
    Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30
    [PMID:22316300]
  36. Hu Y,Dong Q,Yu D
    Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae.
    Plant Sci., 2012. 185-186: p. 288-97
    [PMID:22325892]
  37. Mafra V, et al.
    Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions.
    PLoS ONE, 2012. 7(2): p. e31263
    [PMID:22347455]
  38. Shim JS, et al.
    AtMYB44 regulates WRKY70 expression and modulates antagonistic interaction between salicylic acid and jasmonic acid signaling.
    Plant J., 2013. 73(3): p. 483-95
    [PMID:23067202]
  39. Shim JS,Choi YD
    Direct regulation of WRKY70 by AtMYB44 in plant defense responses.
    Plant Signal Behav, 2013. 8(6): p. e20783
    [PMID:23603962]
  40. Li J, et al.
    Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis.
    New Phytol., 2013. 200(2): p. 457-72
    [PMID:23815736]
  41. Machens F,Becker M,Umrath F,Hehl R
    Identification of a novel type of WRKY transcription factor binding site in elicitor-responsive cis-sequences from Arabidopsis thaliana.
    Plant Mol. Biol., 2014. 84(4-5): p. 371-85
    [PMID:24104863]
  42. Kroes A,van Loon JJ,Dicke M
    Density-dependent interference of aphids with caterpillar-induced defenses in Arabidopsis: involvement of phytohormones and transcription factors.
    Plant Cell Physiol., 2015. 56(1): p. 98-106
    [PMID:25339349]
  43. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]