PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G48590.1
Common NameATHAP5A, HAP5A, NFYC1, NF-YC1, T8P19.100
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family NF-YC
Protein Properties Length: 234aa    MW: 25328.5 Da    PI: 4.832
Description nuclear factor Y, subunit C1
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G48590.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1NF-YC178.27.5e-56431471102
        NF-YC   1 qlksfwekq...iekatdfknhelPlarikkilkadedvkmisaeaPvllskacelfileltlrswlhaeenkrrtlkksdiaaavtrtdifdflvdi 95 
                  ql+ fw++q   ie+++dfknh+lPlarikki+kadedv+misaeaP+l++kacelfilelt+rswlhaeenkrrtl+k+diaaa+trtdifdflvdi
  AT3G48590.1  43 QLQLFWTYQrqeIEQVNDFKNHQLPLARIKKIMKADEDVRMISAEAPILFAKACELFILELTIRSWLHAEENKRRTLQKNDIAAAITRTDIFDFLVDI 140
                  79*****998899************************************************************************************* PP

        NF-YC  96 vprdelk 102
                  vprde+k
  AT3G48590.1 141 VPRDEIK 147
                  ****975 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF471136.04E-3426137IPR009072Histone-fold
PfamPF008081.6E-2065128IPR003958Transcription factor CBF/NF-Y/archaeal histone domain
Gene3DG3DSA:1.10.20.106.9E-3766143IPR009072Histone-fold
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0016602Cellular ComponentCCAAT-binding factor complex
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046982Molecular Functionprotein heterodimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
PO:0009046anatomyflower
PO:0007131developmental stageseedling development stage
Sequence ? help Back to Top
Protein Sequence    Length: 234 aa     Download sequence    Send to blast
MDTNNQQPPP SAAGIPPPPP GTTISAAGGG ASYHHLLQQQ QQQLQLFWTY QRQEIEQVND  60
FKNHQLPLAR IKKIMKADED VRMISAEAPI LFAKACELFI LELTIRSWLH AEENKRRTLQ  120
KNDIAAAITR TDIFDFLVDI VPRDEIKDEA AVLGGGMVVA PTASGVPYYY PPMGQPAGPG  180
GMMIGRPAMD PNGVYVQPPS QAWQSVWQTS TGTGDDVSYG SGGSSGQGNL DGQG
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4g91_C5e-444414319119HapE
4g92_C5e-444414319119HapE
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1115121RRTLQKN
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.230090.0flower
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1865108030.0
Genevisible247388_s_at5e-68
Expression AtlasAT3G48590-
AtGenExpressAT3G48590-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Ubiquitous. Present in etiolated seedlings. {ECO:0000269|PubMed:11250072, ECO:0000269|PubMed:17322342, ECO:0000269|PubMed:9662544}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a protein with similarity to a subunit of the CCAAT promoter motif binding complex of yeast.One of two members of this class (HAP5A) and expressed in vegetative and reproductive tissues.
UniProtStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. {ECO:0000250}.
Function -- GeneRIF ? help Back to Top
  1. AtHAP5A modulates freezing stress resistance in Arabidopsis through binding to the CCAAT motif of AtXTH21.
    [PMID: 24739069]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G48590.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G53340, AT4G14540, AT5G06510, AT5G12840, AT5G15840, AT5G47640, AT5G47670, AT1G09030, AT1G17590, AT1G21970, AT1G28050, AT1G30500, AT1G72830
IntActSearch Q9SMP0
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G48590
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0012060.0BT001206.1 Arabidopsis thaliana transcription factor Hap5a (At3g48590) mRNA, complete cds.
GenBankAY0929970.0AY092997.1 Arabidopsis thaliana transcription factor Hap5a (At3g48590) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_190428.11e-174nuclear transcription factor Y subunit C-1
SwissprotQ9SMP01e-175NFYC1_ARATH; Nuclear transcription factor Y subunit C-1
TrEMBLR0FL351e-150R0FL35_9BRAS; Uncharacterized protein
STRINGAT3G48590.11e-173(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP31517117
MalvidsOGEM47828160
Publications ? help Back to Top
  1. Taira T, et al.
    Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y.
    J. Biol. Chem., 1999. 274(34): p. 24270-9
    [PMID:10446203]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Gusmaroli G,Tonelli C,Mantovani R
    Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana.
    Gene, 2001. 264(2): p. 173-85
    [PMID:11250072]
  4. Gusmaroli G,Tonelli C,Mantovani R
    Regulation of novel members of the Arabidopsis thaliana CCAAT-binding nuclear factor Y subunits.
    Gene, 2002. 283(1-2): p. 41-8
    [PMID:11867211]
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  6. Ben-Naim O, et al.
    The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA.
    Plant J., 2006. 46(3): p. 462-76
    [PMID:16623906]
  7. Wenkel S, et al.
    CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis.
    Plant Cell, 2006. 18(11): p. 2971-84
    [PMID:17138697]
  8. Warpeha KM, et al.
    The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis.
    Plant Physiol., 2007. 143(4): p. 1590-600
    [PMID:17322342]
  9. Yamamoto A, et al.
    Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors.
    Plant J., 2009. 58(5): p. 843-56
    [PMID:19207209]
  10. Hackenberg D, et al.
    Studies on differential nuclear translocation mechanism and assembly of the three subunits of the Arabidopsis thaliana transcription factor NF-Y.
    Mol Plant, 2012. 5(4): p. 876-88
    [PMID:22199235]
  11. Calvenzani V, et al.
    Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
    PLoS ONE, 2012. 7(8): p. e42902
    [PMID:22912760]
  12. Shi H, et al.
    AtHAP5A modulates freezing stress resistance in Arabidopsis through binding to CCAAT motif of AtXTH21.
    New Phytol., 2014. 203(2): p. 554-67
    [PMID:24739069]
  13. Hou X, et al.
    Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis.
    Nat Commun, 2014. 5: p. 4601
    [PMID:25105952]
  14. Edwards D,Murray JA,Smith AG
    Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis.
    Plant Physiol., 1998. 117(3): p. 1015-22
    [PMID:9662544]