PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G25710.1
Common NameATAIG1, BHLH32, EN54, K13N2.1, TMO5
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 344aa    MW: 39496.8 Da    PI: 5.9901
Description basic helix-loop-helix 32
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G25710.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH43.94.2e-14134180354
                  HHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
          HLH   3 rahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                  ++h+e+ErrRR+riN+ +++Lr++lP+      +K +Ka+ L ++++++k+L
  AT3G25710.1 134 KSHSEAERRRRERINTHLAKLRSILPNT-----TKTDKASLLAEVIQHMKEL 180
                  79*************************9.....8****************99 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088816.85131180IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.93E-4134183No hitNo description
PfamPF000101.2E-11134180IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.106.0E-15135191IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474599.55E-15135191IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.3E-13137186IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009718Biological Processanthocyanin-containing compound biosynthetic process
GO:0016036Biological Processcellular response to phosphate starvation
GO:0080147Biological Processroot hair cell development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000026anatomyprimary root tip
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000272anatomyprotoxylem
PO:0000293anatomyguard cell
PO:0008011anatomyembryo vascular system
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 344 aa     Download sequence    Send to blast
MYAMKEEDCL QTFHNLQDYQ DQFHLHHHPQ ILPWSSTSLP SFDPLHFPSN PTRYSDPVHY  60
FNRRASSSSS SFDYNDGFVS PPPSMDHPQN HLRILSEALG PIMRRGSSFG FDGEIMGKLS  120
AQEVMDAKAL AASKSHSEAE RRRRERINTH LAKLRSILPN TTKTDKASLL AEVIQHMKEL  180
KRQTSQITDT YQVPTECDDL TVDSSYNDEE GNLVIRASFC CQDRTDLMHD VINALKSLRL  240
RTLKAEIATV GGRVKNILFL SREYDDEEDH DSYRRNFDGD DVEDYDEERM MNNRVSSIEE  300
ALKAVIEKCV HNNDESNDNN NLEKSSSGGI KRQRTSKMVN RCYN
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1139144ERRRRE
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.221480.0bud| root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306879730.0
Genevisible257642_at1e-179
Expression AtlasAT3G25710-
AtGenExpressAT3G25710-
ATTED-IIAT3G25710-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: At the globular stage, expressed in cells adjacent to the hypophysis and at later embryonic stages, specific for vascular tissues. {ECO:0000269|PubMed:20220754}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a basic helixloophelix transcription factor that is expressed in the hypophysis-adjacent embryo cells, and is required and partially sufficient for MP-dependent root initiation. Involved in response to phosphate starvation. Negative regulator of root hair development, anthocyanin formation and Pi content.
UniProtTranscription factor required for MONOPTEROS-dependent root initiation in embryo. Transcriptionally controlled by MONOPTEROS. {ECO:0000269|PubMed:20220754}.
Function -- GeneRIF ? help Back to Top
  1. TARGET OF MP, TMO5 and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation. [TMO5]
    [PMID: 20220754]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G25710.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT5G42800(R)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G41315
IntActSearch Q9LS08
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No visible phenotype, probably due to redundancy with BHLH30. {ECO:0000269|PubMed:20220754}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G25710
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0588400.0AY058840.1 Arabidopsis thaliana AT3g25710/K13N2_1 mRNA, complete cds.
GenBankAY0790330.0AY079033.1 Arabidopsis thaliana AT3g25710/K13N2_1 mRNA, complete cds.
GenBankAY0863830.0AY086383.1 Arabidopsis thaliana clone 247881 mRNA, complete sequence.
GenBankDQ2056790.0DQ205679.1 Arabidopsis thaliana ABA-regulated protein AIG1 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_189199.10.0basic helix-loop-helix 32
SwissprotQ9LS080.0BH032_ARATH; Transcription factor AIG1
TrEMBLA0A178VA880.0A0A178VA88_ARATH; TMO5
STRINGAT3G25710.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM139491725
Representative plantOGRP37715106
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  3. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Kim J,Kim HY
    Molecular characterization of a bHLH transcription factor involved in Arabidopsis abscisic acid-mediated response.
    Biochim. Biophys. Acta, 2006 Mar-Apr. 1759(3-4): p. 191-4
    [PMID:16730809]
  7. Mace DL, et al.
    Quantification of transcription factor expression from Arabidopsis images.
    Bioinformatics, 2006. 22(14): p. e323-31
    [PMID:16873489]
  8. Chen ZH,Nimmo GA,Jenkins GI,Nimmo HG
    BHLH32 modulates several biochemical and morphological processes that respond to Pi starvation in Arabidopsis.
    Biochem. J., 2007. 405(1): p. 191-8
    [PMID:17376028]
  9. Ohashi-Ito K,Bergmann DC
    Regulation of the Arabidopsis root vascular initial population by LONESOME HIGHWAY.
    Development, 2007. 134(16): p. 2959-68
    [PMID:17626058]
  10. Vald
    Transcriptional regulation and signaling in phosphorus starvation: what about legumes?
    J Integr Plant Biol, 2008. 50(10): p. 1213-22
    [PMID:19017108]
  11. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  12. Schlereth A, et al.
    MONOPTEROS controls embryonic root initiation by regulating a mobile transcription factor.
    Nature, 2010. 464(7290): p. 913-6
    [PMID:20220754]
  13. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  14. Saiga S, et al.
    Control of embryonic meristem initiation in Arabidopsis by PHD-finger protein complexes.
    Development, 2012. 139(8): p. 1391-8
    [PMID:22378640]
  15. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  16. Ohashi-Ito K, et al.
    A bHLH complex activates vascular cell division via cytokinin action in root apical meristem.
    Curr. Biol., 2014. 24(17): p. 2053-8
    [PMID:25131670]
  17. Jost R, et al.
    Differentiating phosphate-dependent and phosphate-independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphite.
    J. Exp. Bot., 2015. 66(9): p. 2501-14
    [PMID:25697796]
  18. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  19. Vera-Sirera F, et al.
    A bHLH-Based Feedback Loop Restricts Vascular Cell Proliferation in Plants.
    Dev. Cell, 2015. 35(4): p. 432-43
    [PMID:26609958]
  20. Katayama H, et al.
    A Negative Feedback Loop Controlling bHLH Complexes Is Involved in Vascular Cell Division and Differentiation in the Root Apical Meristem.
    Curr. Biol., 2015. 25(23): p. 3144-50
    [PMID:26616019]
  21. Ezer D, et al.
    The G-Box Transcriptional Regulatory Code in Arabidopsis.
    Plant Physiol., 2017. 175(2): p. 628-640
    [PMID:28864470]