PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G19580.2
Common NameAZF2, MMB12.27, MMB12.4, ZF2
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family C2H2
Protein Properties Length: 273aa    MW: 29795.7 Da    PI: 8.6023
Description zinc-finger protein 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G19580.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
      zf-C2H2   1 ykCpdCgksFsrksnLkrHirtH 23 
                  ykC+ C k F++   L  H  +H
                  9***********99999998887 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF576671.22E-7105128No hitNo description
Gene3DG3DSA: finger C2H2-type/integrase DNA-binding domain
PROSITE profilePS5015710.887106133IPR007087Zinc finger, C2H2
SMARTSM003550.017106128IPR015880Zinc finger, C2H2-like
PfamPF139127.6E-13106129IPR007087Zinc finger, C2H2
PROSITE patternPS000280108128IPR007087Zinc finger, C2H2
SuperFamilySSF576671.22E-7160187No hitNo description
SMARTSM003550.17165187IPR015880Zinc finger, C2H2-like
PROSITE profilePS501579.099165187IPR007087Zinc finger, C2H2
PfamPF139129.0E-14165189IPR007087Zinc finger, C2H2
PROSITE patternPS000280167187IPR007087Zinc finger, C2H2
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009414Biological Processresponse to water deprivation
GO:0009737Biological Processresponse to abscisic acid
GO:0009738Biological Processabscisic acid-activated signaling pathway
GO:0009793Biological Processembryo development ending in seed dormancy
GO:0010200Biological Processresponse to chitin
GO:0042538Biological Processhyperosmotic salinity response
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008270Molecular Functionzinc ion binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000025anatomyroot tip
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0001054developmental stagevascular leaf senescent stage
PO:0001185developmental stageplant embryo globular stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 273 aa     Download sequence    Send to blast
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.230230.0floral meristem| leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G19580-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, radicles, cotyledons, hypocotyls, leaf veins, stems, sepals, petals, stamens, placenta, funiculi and maturated seeds. {ECO:0000269|PubMed:10806347, ECO:0000269|PubMed:15333755, ECO:0000269|PubMed:20619483}.
Functional Description ? help Back to Top
Source Description
TAIREncodes zinc finger protein. mRNA levels are upregulated in response to ABA, high salt, and mild dessication. The protein is localized to the nucleus and acts as a transcriptional repressor.
UniProtTranscriptional repressor involved in the inhibition of plant growth under abiotic stress conditions. Can repress the expression of various genes, including osmotic stress and abscisic acid-repressive genes and auxin-inducible genes, by binding to their promoter regions in a DNA sequence-specific manner. Acts as a negative regulator of abscisic acid (ABA) signaling during seed germination. Probably involved in jasmonate (JA) early signaling response. May regulate the expression of the JA biosynthesis gene LOX3 and control the expression of TIFY10A/JAZ1, a key repressor in the JA signaling cascade. May act as a positive regulator of leaf senescence. Has been identified as a suppressor of the deficiency of yeast snf4 mutant to grow on non-fermentable carbon source. {ECO:0000269|PubMed:10929106, ECO:0000269|PubMed:15333755, ECO:0000269|PubMed:18216250, ECO:0000269|PubMed:20619483, ECO:0000269|PubMed:21852415, ECO:0000269|PubMed:22709441}.
Function -- GeneRIF ? help Back to Top
  1. Results indicate that AZF2 is a negative regulator of ABA signaling in seeds.
    [PMID: 20619483]
  2. AZF1 and AZF2 function as transcriptional repressors involved in the inhibition of plant growth under abiotic stress conditions.
    [PMID: 21852415]
  3. positive regulator of leaf senescence
    [PMID: 22709441]
  4. Transcript regulation of AtbZIP9 and AtAFZ2 during germination was compatible with the proposed role in transcriptional regulation of AtTrxo1. Transient over-expression of AtbZIP9 and AtAZF2 in Nicotiana benthamiana leaves demonstrated an activation effect of AtbZIP9 and a repressor effect of AtAZF2 on AtTrxo1 promoter-driven reporter expression.
    [PMID: 28184497]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By abscisic acid (ABA), ethylene, salt, cold, dehydration and the monovalent and divalent cations Li(+), Na(+), K(+), Cs(+), Ca(2+) and Mg(2+). {ECO:0000269|PubMed:10806347, ECO:0000269|PubMed:15333755, ECO:0000269|PubMed:20619483, ECO:0000269|PubMed:21852415}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Delayed senescence and hypersensitivity to ABA during seed germination. {ECO:0000269|PubMed:20619483, ECO:0000269|PubMed:22709441}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G19580
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0307300.0AB030730.1 Arabidopsis thaliana AZF2 gene for Cys2/His2-type zinc finger protein 2, complete cds.
GenBankAF2503370.0AF250337.1 Arabidopsis thaliana zinc finger protein AZF2 (AZF2) mRNA, complete cds.
GenBankAP0004170.0AP000417.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MMB12.
GenBankBT0038160.0BT003816.1 Arabidopsis thaliana clone C105170 putative zinc finger protein (At3g19580) mRNA, complete cds.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001118663.10.0zinc-finger protein 2
RefseqNP_188592.10.0zinc-finger protein 2
SwissprotQ9SSW20.0AZF2_ARATH; Zinc finger protein AZF2
TrEMBLA0A178VJ970.0A0A178VJ97_ARATH; ZF2
STRINGAT3G19580.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Sakamoto H,Araki T,Meshi T,Iwabuchi M
    Expression of a subset of the Arabidopsis Cys(2)/His(2)-type zinc-finger protein gene family under water stress.
    Gene, 2000. 248(1-2): p. 23-32
  2. Kleinow T, et al.
    Functional identification of an Arabidopsis snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast.
    Plant J., 2000. 23(1): p. 115-22
  3. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Jiao Y, et al.
    A genome-wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development.
    Plant Physiol., 2003. 133(4): p. 1480-93
  6. Guan Y,Nothnagel EA
    Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures.
    Plant Physiol., 2004. 135(3): p. 1346-66
  7. Englbrecht CC,Schoof H,B
    Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome.
    BMC Genomics, 2004. 5(1): p. 39
  8. Sakamoto H, et al.
    Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions.
    Plant Physiol., 2004. 136(1): p. 2734-46
  9. Hampton CR, et al.
    Cesium toxicity in Arabidopsis.
    Plant Physiol., 2004. 136(3): p. 3824-37
  10. Suzuki N, et al.
    Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c.
    Plant Physiol., 2005. 139(3): p. 1313-22
  11. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
  12. Mandaokar A, et al.
    Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling.
    Plant J., 2006. 46(6): p. 984-1008
  13. Nemhauser JL,Hong F,Chory J
    Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses.
    Cell, 2006. 126(3): p. 467-75
  14. Xu S,Wang X,Chen J
    Zinc finger protein 1 (ThZF1) from salt cress (Thellungiella halophila) is a Cys-2/His-2-type transcription factor involved in drought and salt stress.
    Plant Cell Rep., 2007. 26(4): p. 497-506
  15. Ma S,Bohnert HJ
    Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression.
    Genome Biol., 2007. 8(4): p. R49
  16. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  17. Pauwels L, et al.
    Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells.
    Proc. Natl. Acad. Sci. U.S.A., 2008. 105(4): p. 1380-5
  18. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  19. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
  20. Pauwels L,Goossens A
    Fine-tuning of early events in the jasmonate response.
    Plant Signal Behav, 2008. 3(10): p. 846-7
  21. Drechsel G,Raab S,Hoth S
    Arabidopsis zinc-finger protein 2 is a negative regulator of ABA signaling during seed germination.
    J. Plant Physiol., 2010. 167(16): p. 1418-21
  22. Kodaira KS, et al.
    Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions.
    Plant Physiol., 2011. 157(2): p. 742-56
  23. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
  24. Li Z,Peng J,Wen X,Guo H
    Gene network analysis and functional studies of senescence-associated genes reveal novel regulators of Arabidopsis leaf senescence.
    J Integr Plant Biol, 2012. 54(8): p. 526-39
  25. Schweizer F,Bodenhausen N,Lassueur S,Masclaux FG,Reymond P
    Differential Contribution of Transcription Factors to Arabidopsis thaliana Defense Against Spodoptera littoralis.
    Front Plant Sci, 2013. 4: p. 13
  26. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  27. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  28. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  29. Ortiz-EspĂ­n A, et al.
    Mitochondrial AtTrxo1 is transcriptionally regulated by AtbZIP9 and AtAZF2 and affects seed germination under saline conditions.
    J. Exp. Bot., 2017. 68(5): p. 1025-1038