PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G14230.3
Common NameERF075, MLN21.1, RAP2-2, RAP2.2
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 374aa    MW: 41980.3 Da    PI: 4.6519
Description related to AP2 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G14230.3genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP262.78e-20122171255
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  +y+G+r+++ +g+W+AeIrdp++     r +lg+f+taeeAa+a++aa+++++g
  AT3G14230.3 122 QYRGIRQRP-WGKWAAEIRDPRK---GSREWLGTFDTAEEAARAYDAAARRIRG 171
                  69*******.**********954...39************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5103224.54122179IPR001471AP2/ERF domain
CDDcd000182.43E-17122180No hitNo description
Gene3DG3DSA:3.30.730.102.4E-32122180IPR001471AP2/ERF domain
SuperFamilySSF541712.42E-22122180IPR016177DNA-binding domain
SMARTSM003809.1E-40122185IPR001471AP2/ERF domain
PRINTSPR003673.2E-11123134IPR001471AP2/ERF domain
PfamPF008479.7E-13123171IPR001471AP2/ERF domain
PRINTSPR003673.2E-11145161IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0001666Biological Processresponse to hypoxia
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010468Biological Processregulation of gene expression
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 374 aa     Download sequence    Send to blast
MCGGAIISDF IPPPRSLRVT NEFIWPDLKN KVKASKKRSN KRSDFFDLDD DFEADFQGFK  60
DDSAFDCEDD DDVFVNVKPF VFTATTKPVA SAFVSTGSAY AKKTVESAEQ AEKSSKRKRK  120
NQYRGIRQRP WGKWAAEIRD PRKGSREWLG TFDTAEEAAR AYDAAARRIR GTKAKVNFPE  180
EKNPSVVSQK RPSAKTNNLQ KSVAKPNKSV TLVQQPTHLS QQYCNNSFDN SFGDMSFMEE  240
KPQMYNNQFG LTNSFDAGGN NGYQYFSSDQ GSNSFDCSEF GWSDHGPKTP EISSMLVNNN  300
EASFVEETNA AKKLKPNSDE SDDLMAYLDN ALWDTPLEVE AMLGADAGAV TQEEENPVEL  360
WSLDEINFML EGDF
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A6e-22122179260ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.251460.0bud| flower| leaf| root| seed| silique| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO306830540.0
Genevisible258366_at0.0
Expression AtlasAT3G14230-
AtGenExpressAT3G14230-
ATTED-IIAT3G14230-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Constitutive in flowers, leaves, stems, and roots. {ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:9192694}.
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-2 of ERF/AP2 transcription factor family (RAP2.2). The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.
UniProtTranscription factor involved in carotenoid biosynthesis regulation. Binds to the 5'-ATCTA-3' element present in the promoter of phytoene synthase (PSY) and phytoene desaturase (PDS). Involved in ethylene response and resistance to necrotrophic pathogens. Acts as a downstream regulator in the ethylene signaling pathway. Partially redundant with RAP2-12. {ECO:0000269|PubMed:17873090, ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:22530619}.
Function -- GeneRIF ? help Back to Top
  1. The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription.
    [PMID: 17873090]
  2. Ethylene and RAP2.2, together with oxygen-dependent signal transduction, play an important role in the response to hypoxia.
    [PMID: 20357136]
  3. RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses.
    [PMID: 22530619]
  4. RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation.
    [PMID: 25847219]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G14230.3
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated by darkness, ethylene and Botrytis cinerea. {ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:22530619}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G10585, AT1G53170
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Lethal when homozygous. {ECO:0000269|PubMed:17873090}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G14230
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0003740.0BT000374.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230) mRNA, complete cds.
GenBankAY0545390.0AY054539.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230; MLN21.1) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_850583.10.0ethylene-responsive transcription factor RAP2-2
SwissprotQ9LUM40.0RAP22_ARATH; Ethylene-responsive transcription factor RAP2-2
TrEMBLR0G5V10.0R0G5V1_9BRAS; Uncharacterized protein
STRINGAT3G14230.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Zik M,Irish VF
    Global identification of target genes regulated by APETALA3 and PISTILLATA floral homeotic gene action.
    Plant Cell, 2003. 15(1): p. 207-22
    [PMID:12509532]
  3. Hirai MY, et al.
    Global expression profiling of sulfur-starved Arabidopsis by DNA macroarray reveals the role of O-acetyl-l-serine as a general regulator of gene expression in response to sulfur nutrition.
    Plant J., 2003. 33(4): p. 651-63
    [PMID:12609039]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Hu W,Wang Y,Bowers C,Ma H
    Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis.
    Plant Mol. Biol., 2003. 53(4): p. 545-63
    [PMID:15010618]
  6. Nakayama N, et al.
    Gene trap lines define domains of gene regulation in Arabidopsis petals and stamens.
    Plant Cell, 2005. 17(9): p. 2486-506
    [PMID:16055634]
  7. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  8. Mandaokar A, et al.
    Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling.
    Plant J., 2006. 46(6): p. 984-1008
    [PMID:16805732]
  9. Brodersen P, et al.
    Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4.
    Plant J., 2006. 47(4): p. 532-46
    [PMID:16813576]
  10. Welsch R,Maass D,Voegel T,Dellapenna D,Beyer P
    Transcription factor RAP2.2 and its interacting partner SINAT2: stable elements in the carotenogenesis of Arabidopsis leaves.
    Plant Physiol., 2007. 145(3): p. 1073-85
    [PMID:17873090]
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  12. Hinz M, et al.
    Arabidopsis RAP2.2: an ethylene response transcription factor that is important for hypoxia survival.
    Plant Physiol., 2010. 153(2): p. 757-72
    [PMID:20357136]
  13. Hanada K, et al.
    Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.
    Mol. Biol. Evol., 2011. 28(1): p. 377-82
    [PMID:20736450]
  14. Ou B, et al.
    A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation.
    Mol Plant, 2011. 4(3): p. 546-55
    [PMID:21343311]
  15. Zhao Y, et al.
    Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses.
    New Phytol., 2012. 195(2): p. 450-60
    [PMID:22530619]
  16. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  17. Lumba S, et al.
    A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.
    Dev. Cell, 2014. 29(3): p. 360-72
    [PMID:24823379]
  18. Papdi C, et al.
    The low oxygen, oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3.
    Plant J., 2015. 82(5): p. 772-84
    [PMID:25847219]
  19. Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD
    The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81
    [PMID:9192694]