PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G12250.5
Common NameBZIP45, F28J15.6, MQC3.8, TGA6
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 303aa    MW: 33963.4 Da    PI: 10.0928
Description TGACG motif-binding factor 6
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G12250.5genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_130.86.5e-101959545
                 CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1  5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaL 45
                 k  rr+++NReAAr+sR+RKka++++Le+   +L++  ++L
  AT3G12250.5 19 KTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQEL 59
                 7889************************9877777766555 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.20.5.1705.4E-8962No hitNo description
SMARTSM003381.8E-61578IPR004827Basic-leucine zipper domain
PROSITE profilePS502179.4381761IPR004827Basic-leucine zipper domain
PfamPF001706.1E-71959IPR004827Basic-leucine zipper domain
SuperFamilySSF579594.92E-71962No hitNo description
PROSITE patternPS0003602237IPR004827Basic-leucine zipper domain
PfamPF141441.7E-34101176IPR025422Transcription factor TGA like domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009410Biological Processresponse to xenobiotic stimulus
GO:0009627Biological Processsystemic acquired resistance
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0016787Molecular Functionhydrolase activity
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0046872Molecular Functionmetal ion binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 303 aa     Download sequence    Send to blast
MHAAASDSSD RSKDKLDQKT LRRLAQNREA ARKSRLRKKA YVQQLENSRL KLTQLEQELQ  60
RARQQGVFIS SSGDQAHSTG GNGALAFDAE HSRWLEEKNR QMNELRSALN AHAGDTELRI  120
IVDGVMAHYE ELFRIKSNAA KNDVFHLLSG MWKTPAERCF LWLGGFRSSE LLKLLANQLE  180
PMTERQVMGI NSLQQTSQQA EDALSQGMES LQQSLADTLS SGTLGSSSSD NVASYMGQMA  240
MAMGQLGTLE GFIRQADNLR LQTLQQMLRV LTTRQSARAL LAIHDYSSRL RALSSLWLAR  300
PRE
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.702070.0floral meristem
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible256269_at0.0
Expression AtlasAT3G12250-
AtGenExpressAT3G12250-
ATTED-IIAT3G12250-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed primarily in roots of young seedlings and later expressed in aging cotyledons, mesophyll cells of hydathodes on leaf margins, vascular tissue and trichomes of senescing rosette leaves. Also detected in young lateral roots and in mature pollen grains.
UniprotTISSUE SPECIFICITY: Expressed predominantly in roots and flowers.
Functional Description ? help Back to Top
Source Description
TAIRbasic leucine zipper transcription factor involved in the activation of SA-responsive genes.
UniProtTranscriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters (By similarity). {ECO:0000250}.
Function -- GeneRIF ? help Back to Top
  1. ABF2, an ABF subfamily member of bZIP proteins interacting with the ABA-responsive elements, is involved in Absicic ascid/stress responses. (bZIP) in arabidopsis.
    [PMID: 15361142]
  2. data show TGA factors, which are essential for the salicylic acid (SA)-dependent establishment of systemic acquired resistance, play a pivotal role in the activation of the jasmonic acid/ethylene pathway, both after pathogen infection & hormone treatment
    [PMID: 19832945]
  3. TGA factors 2, 5, and 6 activate oxylipin-responsive gene expression but impede inhibition of root growth by oxylipins.
    [PMID: 23349138]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00349DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G12250.5
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G14610(A), AT5G44420(R)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDsalicylic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch Q39140
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G12250
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ3205400.0AJ320540.1 Arabidopsis thaliana mRNA for transcription factor TGA6 (AtbZIP45 gene).
GenBankBT0295150.0BT029515.1 Arabidopsis thaliana At3g12250 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001078141.10.0TGACG motif-binding factor 6
SwissprotQ391400.0TGA6_ARATH; Transcription factor TGA6
TrEMBLA0A384KPR60.0A0A384KPR6_ARATH; TGA6
TrEMBLA1A6J50.0A1A6J5_ARATH; At3g12250
TrEMBLA8MR700.0A8MR70_ARATH; TGACG motif-binding factor 6
STRINGAT3G12250.40.0(Arabidopsis thaliana)
STRINGXP_006407352.10.0(Eutrema salsugineum)
Publications ? help Back to Top
  1. Zhang Y,Fan W,Kinkema M,Li X,Dong X
    Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.
    Proc. Natl. Acad. Sci. U.S.A., 1999. 96(11): p. 6523-8
    [PMID:10339621]
  2. Zhou JM, et al.
    NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid.
    Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202
    [PMID:10659709]
  3. Després C,DeLong C,Glaze S,Liu E,Fobert PR
    The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors.
    Plant Cell, 2000. 12(2): p. 279-90
    [PMID:10662863]
  4. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  5. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  6. Zhang Y,Tessaro MJ,Lassner M,Li X
    Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance.
    Plant Cell, 2003. 15(11): p. 2647-53
    [PMID:14576289]
  7. Kim S,Kang JY,Cho DI,Park JH,Kim SY
    ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance.
    Plant J., 2004. 40(1): p. 75-87
    [PMID:15361142]
  8. Liu G,Holub EB,Alonso JM,Ecker JR,Fobert PR
    An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.
    Plant J., 2005. 41(2): p. 304-18
    [PMID:15634206]
  9. Hepworth SR,Zhang Y,McKim S,Li X,Haughn GW
    BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis.
    Plant Cell, 2005. 17(5): p. 1434-48
    [PMID:15805484]
  10. Kang HG,Klessig DF
    Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2.
    Plant Mol. Biol., 2005. 57(4): p. 541-57
    [PMID:15821979]
  11. Zhang Y, et al.
    Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs.
    Plant J., 2006. 48(5): p. 647-56
    [PMID:17076807]
  12. Rochon A,Boyle P,Wignes T,Fobert PR,Després C
    The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines.
    Plant Cell, 2006. 18(12): p. 3670-85
    [PMID:17172357]
  13. Popescu SC, et al.
    Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(11): p. 4730-5
    [PMID:17360592]
  14. Kesarwani M,Yoo J,Dong X
    Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis.
    Plant Physiol., 2007. 144(1): p. 336-46
    [PMID:17369431]
  15. Ndamukong I, et al.
    SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription.
    Plant J., 2007. 50(1): p. 128-39
    [PMID:17397508]
  16. Mueller S, et al.
    General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis.
    Plant Cell, 2008. 20(3): p. 768-85
    [PMID:18334669]
  17. Fode B,Siemsen T,Thurow C,Weigel R,Gatz C
    The Arabidopsis GRAS protein SCL14 interacts with class II TGA transcription factors and is essential for the activation of stress-inducible promoters.
    Plant Cell, 2008. 20(11): p. 3122-35
    [PMID:18984675]
  18. Li S, et al.
    Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana.
    Plant Cell, 2009. 21(2): p. 429-41
    [PMID:19218396]
  19. Zander M,La Camera S,Lamotte O,Métraux JP,Gatz C
    Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses.
    Plant J., 2010. 61(2): p. 200-10
    [PMID:19832945]
  20. Matthes MC, et al.
    The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence.
    Planta, 2010. 232(5): p. 1163-80
    [PMID:20711606]
  21. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
    [PMID:22065421]
  22. Zander M,Chen S,Imkampe J,Thurow C,Gatz C
    Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interacting glutaredoxins depends on their C-terminal ALWL motif.
    Mol Plant, 2012. 5(4): p. 831-40
    [PMID:22207719]
  23. Canet JV,Dob
    The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate.
    BMC Plant Biol., 2012. 12: p. 199
    [PMID:23116333]
  24. Stotz HU,Mueller S,Zoeller M,Mueller MJ,Berger S
    TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins.
    J. Exp. Bot., 2013. 64(4): p. 963-75
    [PMID:23349138]
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  26. Xiang C,Miao Z,Lam E
    DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.
    Plant Mol. Biol., 1997. 34(3): p. 403-15
    [PMID:9225852]