PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G06590.2
Common NameAIF2, BHLH148, EN143, F5E6.8
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 221aa    MW: 24256 Da    PI: 12.3375
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G06590.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH14.47.1e-051641981655
                  HHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH  16 iNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  +N +   L +l+P +     +K s   iLe+A++YI+ L+
  AT3G06590.2 164 VNRKVRVLGRLVPGC-----GKQSVPVILEEATDYIQALE 198
                  566667778888888.....9****************985 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474596.19E-9145213IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088811.077148197IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.108.0E-7163207IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 221 aa     Download sequence    Send to blast
MASLISDIEP PTSTTSDLVR RKKRSSASSA ASSRSSASSV SGEIHARWRS EKQQRIYSAK  60
LFQALQQVRL NSSASTSSSP TAQKRGKAVR EAADRALAVS ARGRTLWSRA ILANRIKLKF  120
RKQRRPRATM AIPAMTTVVS SSSNRSRKRR VSVLRLNKKS IPDVNRKVRV LGRLVPGCGK  180
QSVPVILEEA TDYIQALEMQ VRAMNSLVQL LSSYGSAPPP I
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.252330.0flower| root| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible258511_at0.0
Expression AtlasAT3G06590-
AtGenExpressAT3G06590-
ATTED-IIAT3G06590-
Functional Description ? help Back to Top
Source Description
UniProtbHLH transcription factor that binds DNA on specific sequence 5'-CANNTG-3' in target gene promoters (PubMed:24009530). Negatively regulates brassinosteroid signaling (PubMed:20023194). Together with BHLH148/RITF1, regulates the transcription of several genes involved in the detoxification of reactive oxygen species (ROS) generated by salt (NaCl) stress. Confers tolerance to salt and to the oxidative stress-inducing reagents hydrogen peroxide H(2)O(2) and methyl viologen (MV) (PubMed:24009530). {ECO:0000269|PubMed:20023194, ECO:0000269|PubMed:24009530}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G06590.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Slightly induced by salt (NaCl) stress. {ECO:0000269|PubMed:24009530}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G34530, AT4G36540, AT5G15160, AT1G59640
IntActSearch Q9C8Z9
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G06590
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC0205800.0AC020580.11 Arabidopsis thaliana chromosome 3 BAC F5E6 genomic sequence, complete sequence.
GenBankAY0812880.0AY081288.1 Arabidopsis thaliana unknown protein (At3g06590) mRNA, complete cds.
GenBankAY0933900.0AY093390.1 Arabidopsis thaliana unknown protein (At3g06590) mRNA, complete cds.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001325894.11e-154basic helix-loop-helix (bHLH) DNA-binding superfamily protein
RefseqNP_001325895.11e-154basic helix-loop-helix (bHLH) DNA-binding superfamily protein
RefseqNP_566287.11e-154basic helix-loop-helix (bHLH) DNA-binding superfamily protein
RefseqNP_974239.11e-154basic helix-loop-helix (bHLH) DNA-binding superfamily protein
SwissprotQ9C8Z91e-156BH148_ARATH; Transcription factor bHLH148
TrEMBLA0A178VAS21e-152A0A178VAS2_ARATH; AIF2
STRINGAT3G06590.21e-154(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  2. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  3. Dal Bosco C, et al.
    Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana.
    J. Biol. Chem., 2004. 279(2): p. 1060-9
    [PMID:14576160]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  6. Fizames C, et al.
    The Arabidopsis root transcriptome by serial analysis of gene expression. Gene identification using the genome sequence.
    Plant Physiol., 2004. 134(1): p. 67-80
    [PMID:14730065]
  7. Wagner R,Pfannschmidt T
    Eukaryotic transcription factors in plastids--Bioinformatic assessment and implications for the evolution of gene expression machineries in plants.
    Gene, 2006. 381: p. 62-70
    [PMID:16934950]
  8. Wang H, et al.
    Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins.
    Plant Cell, 2009. 21(12): p. 3781-91
    [PMID:20023194]
  9. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  10. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  11. Ikeda M,Mitsuda N,Ohme-Takagi M
    ATBS1 INTERACTING FACTORs negatively regulate Arabidopsis cell elongation in the triantagonistic bHLH system.
    Plant Signal Behav, 2013. 8(3): p. e23448
    [PMID:23333962]
  12. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  13. Guan Q,Wu J,Yue X,Zhang Y,Zhu J
    A nuclear calcium-sensing pathway is critical for gene regulation and salt stress tolerance in Arabidopsis.
    PLoS Genet., 2013. 9(8): p. e1003755
    [PMID:24009530]
  14. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  15. Sukweenadhi J, et al.
    Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress.
    Microbiol. Res., 2015. 172: p. 7-15
    [PMID:25721473]