PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G46400.1
Common NameATWRKY46, F11C10.9, WRKY46
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 295aa    MW: 33634.7 Da    PI: 5.9371
Description WRKY DNA-binding protein 46
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G46400.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                  dDg++WrKYGqKe++gs++pr+YYrCt++   +C + k+v++s++dp+++e++Y g+H+++
                  8***************************9999***************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA: domain
PROSITE profilePS5081121.02398161IPR003657WRKY domain
SuperFamilySSF1182904.97E-2498164IPR003657WRKY domain
SMARTSM007746.1E-36103165IPR003657WRKY domain
PfamPF031066.5E-23104163IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010200Biological Processresponse to chitin
GO:0048527Biological Processlateral root development
GO:0005730Cellular Componentnucleolus
GO:0000987Molecular Functioncore promoter proximal region sequence-specific DNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 295 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.265100.0root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT2G46400-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root (PubMed:24773321). Mostly expressed in roots, at lower levels in leaves and petioles, and, to a lower extent, in stems, flowers and siliques (PubMed:22325892). {ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321}.
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group III
UniProtTranscription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). {ECO:0000250, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321, ECO:0000269|PubMed:28576847}.
Function -- GeneRIF ? help Back to Top
  1. WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and regulate salicylic acid synthesis upon pathogen attack. [WRKY46]
    [PMID: 21595875]
  2. WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense.
    [PMID: 22325892]
  3. WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. Mutation of WRKY46 leads to increased Al resistance.
    [PMID: 24118304]
  4. WRKY46 plays dual roles in regulating plant responses to drought and salt stress and light-dependent stomatal opening in guard cells.
    [PMID: 24773321]
  5. results demonstrate that WRKY46 contributes to the feedforward inhibition of osmotic/salt stress-dependent LR inhibition via regulation of ABA signaling and auxin homeostasis
    [PMID: 26252246]
  6. WRKY46 plays an important role in the control of root-to-shoot iron translocation under iron deficiency condition via direct regulation of VITL1 transcript levels.WRKY46 binds to the promoter region of VITL1.
    [PMID: 27208259]
  7. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses
    [PMID: 28576847]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments (PubMed:24773321). Induced by salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora (PubMed:22316300). Triggered by P.syringae (PubMed:22325892). {ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Decreased tolerance to dehydration and salt stress. {ECO:0000269|PubMed:24773321}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G46400
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0462750.0AY046275.1 Arabidopsis thaliana WRKY transcription factor 46 (WRKY46) mRNA, complete cds.
GenBankAY0808120.0AY080812.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.
GenBankAY1172100.0AY117210.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_182163.10.0WRKY DNA-binding protein 46
SwissprotQ9SKD90.0WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBLA0A178W0500.0A0A178W050_ARATH; WRKY46
STRINGAT2G46400.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  4. Zhu J, et al.
    An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2004. 101(26): p. 9873-8
  5. Guan Y,Nothnagel EA
    Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures.
    Plant Physiol., 2004. 135(3): p. 1346-66
  6. Hass C, et al.
    The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis.
    EMBO J., 2004. 23(16): p. 3290-302
  7. Chang S,Pikaard CS
    Transcript profiling in Arabidopsis reveals complex responses to global inhibition of DNA methylation and histone deacetylation.
    J. Biol. Chem., 2005. 280(1): p. 796-804
  8. Koroleva OA,Tomlinson ML,Leader D,Shaw P,Doonan JH
    High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions.
    Plant J., 2005. 41(1): p. 162-74
  9. Lee BH,Henderson DA,Zhu JK
    The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell, 2005. 17(11): p. 3155-75
  10. Suzuki N, et al.
    Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c.
    Plant Physiol., 2005. 139(3): p. 1313-22
  11. Taki N, et al.
    12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis.
    Plant Physiol., 2005. 139(3): p. 1268-83
  12. Stein M, et al.
    Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration.
    Plant Cell, 2006. 18(3): p. 731-46
  13. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
  14. He P, et al.
    Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity.
    Cell, 2006. 125(3): p. 563-75
  15. Mosher RA,Durrant WE,Wang D,Song J,Dong X
    A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.
    Plant Cell, 2006. 18(7): p. 1750-65
  16. Tosti N, et al.
    Gene expression profiles of O3-treated Arabidopsis plants.
    Plant Cell Environ., 2006. 29(9): p. 1686-702
  17. Lee DJ, et al.
    Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
    Mol. Genet. Genomics, 2007. 277(2): p. 115-37
  18. Qutob D, et al.
    Phytotoxicity and innate immune responses induced by Nep1-like proteins.
    Plant Cell, 2006. 18(12): p. 3721-44
  19. Lee KP,Kim C,Landgraf F,Apel K
    EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from the plastid to the nucleus of Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(24): p. 10270-5
  20. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  21. Chawade A,Br
    Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors.
    BMC Genomics, 2007. 8: p. 304
  22. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  23. van Verk MC,Bol JF,Linthorst HJ
    WRKY transcription factors involved in activation of SA biosynthesis genes.
    BMC Plant Biol., 2011. 11: p. 89
  24. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
  25. Moreau M, et al.
    EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora.
    Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30
  26. Hu Y,Dong Q,Yu D
    Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae.
    Plant Sci., 2012. 185-186: p. 288-97
  27. Renault H, et al.
    γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots.
    Plant Cell Environ., 2013. 36(5): p. 1009-18
  28. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  29. Ding ZJ,Yan JY,Xu XY,Li GX,Zheng SJ
    WRKY46 functions as a transcriptional repressor of ALMT1, regulating aluminum-induced malate secretion in Arabidopsis.
    Plant J., 2013. 76(5): p. 825-35
  30. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  31. Ding ZJ, et al.
    Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis.
    Plant J., 2014. 79(1): p. 13-27
  32. Ding ZJ, et al.
    Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis.
    Plant J., 2015. 84(1): p. 56-69
  33. Sheikh AH, et al.
    Regulation of WRKY46 Transcription Factor Function by Mitogen-Activated Protein Kinases in Arabidopsis thaliana.
    Front Plant Sci, 2016. 7: p. 61
  34. Yan JY, et al.
    A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency.
    Plant Physiol., 2016. 171(3): p. 2017-27
  35. Chen J, et al.
    Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses.
    Plant Cell, 2017. 29(6): p. 1425-1439
  36. Ma J, et al.
    Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana.
    Front Plant Sci, 2017. 8: p. 2036