PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G45420.1
Common NameASL20, F4L23.7, LBD18
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family LBD
Protein Properties Length: 262aa    MW: 27218.6 Da    PI: 8.0852
Description LOB domain-containing protein 18
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G45420.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1DUF260128.53e-40371371100
       DUF260   1 aCaaCkvlrrkCakdCvlapyfpaeq.pkkfanvhklFGasnvlkllkalpeeeredamsslvyeAearardPvyGavgvilklqqqleqlkaelall 97 
                  +C aCk+lrrkC+++C++apyf +eq ++ fa+vhk+FGasnv+kll ++p ++r+da+ +++yeA+ar+rdP+yG+v++i++lqqq+ +l+ae+++l
  AT2G45420.1  37 PCGACKFLRRKCVPGCIFAPYFDSEQgSAYFAAVHKVFGASNVSKLLLHIPVHRRSDAVVTICYEAQARIRDPIYGCVAHIFALQQQVVNLQAEVSYL 134
                  7***********************998899******************************************************************99 PP

       DUF260  98 kee 100
                  +++
  AT2G45420.1 135 QAH 137
                  986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PIRSFPIRSF0381276.8E-1391262IPR017394LOB domain-containing protein 18/20
PROSITE profilePS5089122.86636138IPR004883Lateral organ boundaries, LOB
PfamPF031952.9E-3937135IPR004883Lateral organ boundaries, LOB
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010089Biological Processxylem development
GO:0010311Biological Processlateral root formation
GO:0005634Cellular Componentnucleus
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000084anatomyplant sperm cell
PO:0000290anatomytracheary element
Sequence ? help Back to Top
Protein Sequence    Length: 262 aa     Download sequence    Send to blast
MSGGGNTITA VGGGGGGCGG GGSSGGGGSS GGGGGGPCGA CKFLRRKCVP GCIFAPYFDS  60
EQGSAYFAAV HKVFGASNVS KLLLHIPVHR RSDAVVTICY EAQARIRDPI YGCVAHIFAL  120
QQQVVNLQAE VSYLQAHLAS LELPQPQTRP QPMPQPQPLF FTPPPPLAIT DLPASVSPLP  180
STYDLASIFD QTTSSSAWAT QQRRFIDPRH QYGVSSSSSS VAVGLGGENS HDLQALAHEL  240
LHRQGSPPPA ATDHSPSRTM SR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.213650.0seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO425704260.0
Genevisible245140_at0.0
Expression AtlasAT2G45420-
AtGenExpressAT2G45420-
ATTED-IIAT2G45420-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: During lateral root formation, expressed in the lateral root primordia, and the developing, emerged, and mature lateral roots. {ECO:0000269|PubMed:19717544}.
UniprotTISSUE SPECIFICITY: Expressed in roots, stems, leaves and flowers (PubMed:12068116, PubMed:24484953). Expressed in vascular tissues of hypocotyls, leaves, roots, developing floral organs and siliques (PubMed:19088331). {ECO:0000269|PubMed:12068116, ECO:0000269|PubMed:19088331, ECO:0000269|PubMed:24484953}.
Functional Description ? help Back to Top
Source Description
UniProtInvolved in the positive regulation of tracheary element (TE) differentiation. Involved in a positive feedback loop that maintains or promotes NAC030/VND7 expression that regulates TE differentiation-related genes (PubMed:19088331). Functions in the initiation and emergence of lateral roots, in conjunction with LBD16, downstream of ARF7 and ARF19 (PubMed:19717544, PubMed:23749813). Transcriptional activator that directly regulates EXPA14, a gene encoding a cell wall-loosening factor that promotes lateral root emergence. Activates EXPA14 by directly binding to a specific region of its promoter (PubMed:22974309). Transcriptional activator that directly regulates EXPA17, a gene encoding a cell wall-loosening factor that promotes lateral root emergence (PubMed:23872272). Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development (PubMed:26059335). {ECO:0000269|PubMed:19088331, ECO:0000269|PubMed:19717544, ECO:0000269|PubMed:22974309, ECO:0000269|PubMed:23749813, ECO:0000269|PubMed:23872272, ECO:0000269|PubMed:26059335}.
Function -- GeneRIF ? help Back to Top
  1. LBD16 and LBD18 might function in a combinatorial manner in lateral root formation.
    [PMID: 19717544]
  2. E2Fa expression is regulated by the LATERAL ORGAN BOUNDARY DOMAIN18/LATERAL ORGAN BOUNDARY DOMAIN33 (LBD18/LBD33) dimer that is, in turn, regulated by the auxin signaling pathway.
    [PMID: 22003076]
  3. Data show that overexpression of each of the LBD genes promotes callus formation in the absence of exogenous phytohormones, while suppression of LBD function inhibits the callus induction process.
    [PMID: 22508267]
  4. LBD16, LBD29, and LBD18 are involved in cell cycle progression of the pericycle in response to auxin.
    [PMID: 22699776]
  5. This study revealed the molecular function of LBD18 as a specific DNA-binding transcription factor that activates EXP14 expression by directly binding to its promoter.
    [PMID: 22974309]
  6. the proline mutation completely abolished LBD18 DNA-binding activity.
    [PMID: 23430048]
  7. PUCHI co-acts with LBD16 and LBD18 to control lateral root primordium development and lateral root emergence.
    [PMID: 23749813]
  8. Results suggest that LBD18 up-regulates EXPA17 to enhance cell separation to promote lateral roots (LRs) emergence in Arabidopsis.
    [PMID: 23872272]
  9. GBF INTERACTING PROTEIN1 (GIP1) interacts with LBD18 in yeast and Arabidopsis protoplasts. Reverse-transcription-pcr analysis showed overlapping expression of GIP1 and LBD18 in roots, aerial parts, and rosette leaves of Arabidopsis.
    [PMID: 24484953]
  10. Results suggest that Lateral Organ Boundaries Domain16 and 18 (LBD16 and LBD18) are important regulators of lateral root (LR) initiation and development downstream of AUXIN1 (AUX1) and LIKE-AUXIN3 (LAX3) auxin influx carriers.
    [PMID: 26059335]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00314DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G45420.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By auxin. {ECO:0000269|PubMed:15659631, ECO:0000269|PubMed:19717544, ECO:0000269|PubMed:23749813}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT5G20730 (A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDauxin
Interaction ? help Back to Top
Source Intact With
BioGRIDAT2G45420
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Reduced number of lateral roots. {ECO:0000269|PubMed:19717544}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G45420
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0257160.0BT025716.1 Arabidopsis thaliana At2g45420 mRNA, complete cds.
GenBankAB4738530.0AB473853.1 Arabidopsis thaliana ASL20 mRNA for ASYMMETRIC LEAVES2-like 20 protein, complete cds.
GenBankAF4478910.0AF447891.1 Arabidopsis thaliana LOB DOMAIN 18 (LBD18) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_850436.10.0LOB domain-containing protein 18
SwissprotO221310.0LBD18_ARATH; LOB domain-containing protein 18
TrEMBLV4N2111e-143V4N211_EUTSA; Uncharacterized protein
STRINGAT2G45420.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP40515100
MalvidsOGEM10828359
Publications ? help Back to Top
  1. Iwakawa H, et al.
    The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper.
    Plant Cell Physiol., 2002. 43(5): p. 467-78
    [PMID:12040093]
  2. Shuai B,Reynaga-Peña CG,Springer PS
    The lateral organ boundaries gene defines a novel, plant-specific gene family.
    Plant Physiol., 2002. 129(2): p. 747-61
    [PMID:12068116]
  3. Goda H, et al.
    Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis.
    Plant Physiol., 2004. 134(4): p. 1555-73
    [PMID:15047898]
  4. Redman JC,Haas BJ,Tanimoto G,Town CD
    Development and evaluation of an Arabidopsis whole genome Affymetrix probe array.
    Plant J., 2004. 38(3): p. 545-61
    [PMID:15086809]
  5. Okushima Y, et al.
    Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.
    Plant Cell, 2005. 17(2): p. 444-63
    [PMID:15659631]
  6. Soyano T,Thitamadee S,Machida Y,Chua NH
    ASYMMETRIC LEAVES2-LIKE19/LATERAL ORGAN BOUNDARIES DOMAIN30 and ASL20/LBD18 regulate tracheary element differentiation in Arabidopsis.
    Plant Cell, 2008. 20(12): p. 3359-73
    [PMID:19088331]
  7. Matsumura Y,Iwakawa H,Machida Y,Machida C
    Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and molecular comparisons between AS2 and other family members.
    Plant J., 2009. 58(3): p. 525-37
    [PMID:19154202]
  8. Lee DJ,Park JW,Lee HW,Kim J
    Genome-wide analysis of the auxin-responsive transcriptome downstream of iaa1 and its expression analysis reveal the diversity and complexity of auxin-regulated gene expression.
    J. Exp. Bot., 2009. 60(13): p. 3935-57
    [PMID:19654206]
  9. Lee HW,Kim NY,Lee DJ,Kim J
    LBD18/ASL20 regulates lateral root formation in combination with LBD16/ASL18 downstream of ARF7 and ARF19 in Arabidopsis.
    Plant Physiol., 2009. 151(3): p. 1377-89
    [PMID:19717544]
  10. Berckmans B, et al.
    Auxin-dependent cell cycle reactivation through transcriptional regulation of Arabidopsis E2Fa by lateral organ boundary proteins.
    Plant Cell, 2011. 23(10): p. 3671-83
    [PMID:22003076]
  11. Fan M,Xu C,Xu K,Hu Y
    LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration.
    Cell Res., 2012. 22(7): p. 1169-80
    [PMID:22508267]
  12. Feng Z,Zhu J,Du X,Cui X
    Effects of three auxin-inducible LBD members on lateral root formation in Arabidopsis thaliana.
    Planta, 2012. 236(4): p. 1227-37
    [PMID:22699776]
  13. Lee HW,Kim MJ,Kim NY,Lee SH,Kim J
    LBD18 acts as a transcriptional activator that directly binds to the EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis.
    Plant J., 2013. 73(2): p. 212-24
    [PMID:22974309]
  14. Osakabe Y, et al.
    Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis.
    Plant Cell, 2013. 25(2): p. 609-24
    [PMID:23396830]
  15. Lee HW,Kim MJ,Park MY,Han KH,Kim J
    The conserved proline residue in the LOB domain of LBD18 is critical for DNA-binding and biological function.
    Mol Plant, 2013. 6(5): p. 1722-5
    [PMID:23430048]
  16. Kang NY,Lee HW,Kim J
    The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20 downstream of ARF7 and ARF19 to regulate lateral root development in Arabidopsis.
    Plant Cell Physiol., 2013. 54(8): p. 1326-34
    [PMID:23749813]
  17. Lee HW,Kim J
    EXPANSINA17 up-regulated by LBD18/ASL20 promotes lateral root formation during the auxin response.
    Plant Cell Physiol., 2013. 54(10): p. 1600-11
    [PMID:23872272]
  18. Lee HW,Park JH,Park MY,Kim J
    GIP1 may act as a coactivator that enhances transcriptional activity of LBD18 in Arabidopsis.
    J. Plant Physiol., 2014. 171(5): p. 14-8
    [PMID:24484953]
  19. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  20. Lee HW,Cho C,Kim J
    Lateral Organ Boundaries Domain16 and 18 Act Downstream of the AUXIN1 and LIKE-AUXIN3 Auxin Influx Carriers to Control Lateral Root Development in Arabidopsis.
    Plant Physiol., 2015. 168(4): p. 1792-806
    [PMID:26059335]