PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G42430.1
Common NameASL18, LBD16, MHK10.15
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family LBD
Protein Properties Length: 245aa    MW: 26465.4 Da    PI: 7.865
Description lateral organ boundaries-domain 16
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G42430.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1DUF2601223.1e-38151151100
       DUF260   1 aCaaCkvlrrkCakdCvlapyfpaeq.pkkfanvhklFGasnvlkllkalpeeeredamsslvyeAearardPvyGavgvilklqqqleqlkaelall 97 
                  +C aCk+lrrkCa+dC++apyf +eq +++fa++hk+FGasnv+kll ++p ++r +a+ +++yeA+ar++dPvyG+v++i++lqqq++ l++++ ++
  AT2G42430.1  15 PCGACKFLRRKCASDCIFAPYFSSEQgAARFAAIHKVFGASNVSKLLLNVPIHDRCEAVVTIAYEAQARLHDPVYGCVSHIFALQQQVAFLQSQVMQM 112
                  7***********************9989******************************************************************9999 PP

       DUF260  98 kee 100
                  k++
  AT2G42430.1 113 KAQ 115
                  887 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5089122.86614116IPR004883Lateral organ boundaries, LOB
PfamPF031954.2E-3715113IPR004883Lateral organ boundaries, LOB
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010311Biological Processlateral root formation
GO:0005634Cellular Componentnucleus
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
Sequence ? help Back to Top
Protein Sequence    Length: 245 aa     Download sequence    Send to blast
MASSGNGTTA GTGSPCGACK FLRRKCASDC IFAPYFSSEQ GAARFAAIHK VFGASNVSKL  60
LLNVPIHDRC EAVVTIAYEA QARLHDPVYG CVSHIFALQQ QVAFLQSQVM QMKAQIAGHQ  120
TSAAGDLRHS SESTNQFMTW QQTSVSPIGS AYSTPYNHHQ PYYGHVNPNN PVSPQSSLEE  180
SFSNTSSDVT TTANVRETHH QTGGGVYGHD GIGFHEGYPN KKRSVSYCSS DLGELQALAL  240
RMMKN
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.133750.0root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible265856_at0.0
Expression AtlasAT2G42430-
AtGenExpressAT2G42430-
ATTED-IIAT2G42430-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: During lateral root formation, expressed in the lateral root primordia, and the developing, emerged, and mature lateral roots. {ECO:0000269|PubMed:19717544}.
UniprotTISSUE SPECIFICITY: Expressed in roots and faintly in shoots. {ECO:0000269|PubMed:12068116}.
Functional Description ? help Back to Top
Source Description
TAIRLOB-domain protein gene LBD16. This gene contains one auxin-responsive element (AuxRE).
UniProtTranscriptional activator (PubMed:19717544, PubMed:22974309). Involved in lateral root formation. Regulated by the transcriptional activators ARF7 and ARF19 (PubMed:17259263). Functions in the initiation and emergence of lateral roots, in conjunction with LBD18, downstream of ARF7 and ARF19 (PubMed:19717544, PubMed:23749813). Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development (PubMed:26059335). {ECO:0000269|PubMed:17259263, ECO:0000269|PubMed:19717544, ECO:0000269|PubMed:22974309, ECO:0000269|PubMed:23749813, ECO:0000269|PubMed:26059335}.
Function -- GeneRIF ? help Back to Top
  1. LBD16 and LBD18 might function in a combinatorial manner in lateral root formation.
    [PMID: 19717544]
  2. Results indicate that the localized activity of LBD16/ASL18 and its related LBD/ASLs is involved in the symmetry breaking of lateral root (LR) founder cells for LR initiation, a key step for constructing the plant root system.
    [PMID: 22278921]
  3. Data show that overexpression of each of the LBD genes promotes callus formation in the absence of exogenous phytohormones, while suppression of LBD function inhibits the callus induction process.
    [PMID: 22508267]
  4. LBD16, LBD29, and LBD18 are involved in cell cycle progression of the pericycle in response to auxin.
    [PMID: 22699776]
  5. PUCHI co-acts with LBD16 and LBD18 to control lateral root primordium development and lateral root emergence.
    [PMID: 23749813]
  6. LBD16 is a key molecular player in the xylem pole pericycle cells during giant cells and gall development.
    [PMID: 24803293]
  7. Results suggest that Lateral Organ Boundaries Domain16 and 18 (LBD16 and LBD18) are important regulators of lateral root (LR) initiation and development downstream of AUXIN1 (AUX1) and LIKE-AUXIN3 (LAX3) auxin influx carriers.
    [PMID: 26059335]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00309DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G42430.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By auxin. {ECO:0000269|PubMed:15659631, ECO:0000269|PubMed:17259263, ECO:0000269|PubMed:23749813}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G19220 (A), AT5G20730 (A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDauxin
Interaction ? help Back to Top
Source Intact With
BioGRIDAT2G42430, AT2G45420
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Reduced number of lateral roots. {ECO:0000269|PubMed:19717544}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G42430
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0008660.0BT000866.1 Arabidopsis thaliana At2g42430/MHK10.15 mRNA, complete cds.
GenBankAB4738510.0AB473851.1 Arabidopsis thaliana ASL18 mRNA for ASYMMETRIC LEAVES2-like 18 protein, complete cds.
GenBankAF4478900.0AF447890.1 Arabidopsis thaliana LOB DOMAIN 16 (LBD16) mRNA, complete cds.
GenBankAF4103340.0AF410334.1 Arabidopsis thaliana At2g42430/MHK10.15 mRNA, complete cds.
GenBankAF3453390.0AF345339.1 Arabidopsis thaliana MHK10.15/At2g42430 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_565973.10.0LOB domain-containing protein 16
SwissprotQ9SLB70.0LBD16_ARATH; LOB domain-containing protein 16
TrEMBLD7LI591e-176D7LI59_ARALL; Putative uncharacterized protein
STRINGAT2G42430.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP40515100
MalvidsOGEM10828359
Publications ? help Back to Top
  1. Iwakawa H, et al.
    The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper.
    Plant Cell Physiol., 2002. 43(5): p. 467-78
    [PMID:12040093]
  2. Shuai B,Reynaga-Peña CG,Springer PS
    The lateral organ boundaries gene defines a novel, plant-specific gene family.
    Plant Physiol., 2002. 129(2): p. 747-61
    [PMID:12068116]
  3. Xiao YL,Malik M,Whitelaw CA,Town CD
    Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2 of Arabidopsis.
    Plant Physiol., 2002. 130(4): p. 2118-28
    [PMID:12481096]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Goda H, et al.
    Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis.
    Plant Physiol., 2004. 134(4): p. 1555-73
    [PMID:15047898]
  6. Okushima Y, et al.
    Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.
    Plant Cell, 2005. 17(2): p. 444-63
    [PMID:15659631]
  7. Laplaze L, et al.
    GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana.
    J. Exp. Bot., 2005. 56(419): p. 2433-42
    [PMID:16043452]
  8. Okushima Y,Fukaki H,Onoda M,Theologis A,Tasaka M
    ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis.
    Plant Cell, 2007. 19(1): p. 118-30
    [PMID:17259263]
  9. Matsumura Y,Iwakawa H,Machida Y,Machida C
    Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and molecular comparisons between AS2 and other family members.
    Plant J., 2009. 58(3): p. 525-37
    [PMID:19154202]
  10. Lee HW,Kim NY,Lee DJ,Kim J
    LBD18/ASL20 regulates lateral root formation in combination with LBD16/ASL18 downstream of ARF7 and ARF19 in Arabidopsis.
    Plant Physiol., 2009. 151(3): p. 1377-89
    [PMID:19717544]
  11. Ikeyama Y,Tasaka M,Fukaki H
    RLF, a cytochrome b(5)-like heme/steroid binding domain protein, controls lateral root formation independently of ARF7/19-mediated auxin signaling in Arabidopsis thaliana.
    Plant J., 2010. 62(5): p. 865-75
    [PMID:20230485]
  12. Goh T,Joi S,Mimura T,Fukaki H
    The establishment of asymmetry in Arabidopsis lateral root founder cells is regulated by LBD16/ASL18 and related LBD/ASL proteins.
    Development, 2012. 139(5): p. 883-93
    [PMID:22278921]
  13. Fan M,Xu C,Xu K,Hu Y
    LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration.
    Cell Res., 2012. 22(7): p. 1169-80
    [PMID:22508267]
  14. Kim MJ,Kim J
    Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis.
    J. Plant Physiol., 2012. 169(12): p. 1221-6
    [PMID:22591857]
  15. Feng Z,Zhu J,Du X,Cui X
    Effects of three auxin-inducible LBD members on lateral root formation in Arabidopsis thaliana.
    Planta, 2012. 236(4): p. 1227-37
    [PMID:22699776]
  16. Lee HW,Kim MJ,Kim NY,Lee SH,Kim J
    LBD18 acts as a transcriptional activator that directly binds to the EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis.
    Plant J., 2013. 73(2): p. 212-24
    [PMID:22974309]
  17. M
    Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling.
    J. Exp. Bot., 2012. 63(18): p. 6325-34
    [PMID:23095998]
  18. Kim J,Lee HW
    Direct activation of EXPANSIN14 by LBD18 in the gene regulatory network of lateral root formation in Arabidopsis.
    Plant Signal Behav, 2013. 8(2): p. e22979
    [PMID:23299420]
  19. Lee HW,Kim MJ,Park MY,Han KH,Kim J
    The conserved proline residue in the LOB domain of LBD18 is critical for DNA-binding and biological function.
    Mol Plant, 2013. 6(5): p. 1722-5
    [PMID:23430048]
  20. Kang NY,Lee HW,Kim J
    The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20 downstream of ARF7 and ARF19 to regulate lateral root development in Arabidopsis.
    Plant Cell Physiol., 2013. 54(8): p. 1326-34
    [PMID:23749813]
  21. Cabrera J, et al.
    A role for LATERAL ORGAN BOUNDARIES-DOMAIN 16 during the interaction Arabidopsis-Meloidogyne spp. provides a molecular link between lateral root and root-knot nematode feeding site development.
    New Phytol., 2014. 203(2): p. 632-45
    [PMID:24803293]
  22. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  23. Lee HW,Cho C,Kim J
    Lateral Organ Boundaries Domain16 and 18 Act Downstream of the AUXIN1 and LIKE-AUXIN3 Auxin Influx Carriers to Control Lateral Root Development in Arabidopsis.
    Plant Physiol., 2015. 168(4): p. 1792-806
    [PMID:26059335]