PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G40750.1
Common NameATWRKY54, T7D17.7, WRKY54
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 346aa    MW: 38645.3 Da    PI: 5.1196
Description WRKY DNA-binding protein 54
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G40750.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                  +D y+WrKYGqKe+ +++fprsY+rCt++   gC+++k+v+++++d ++++itY g H+++
                  7***************************9999***************************96 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA: domain
SuperFamilySSF1182907.72E-24145212IPR003657WRKY domain
PROSITE profilePS5081120.266146214IPR003657WRKY domain
SMARTSM007744.7E-36151213IPR003657WRKY domain
PfamPF031061.8E-24152211IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009751Biological Processresponse to salicylic acid
GO:0031347Biological Processregulation of defense response
GO:1900056Biological Processnegative regulation of leaf senescence
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 346 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5w3x_B2e-181462091174Disease resistance protein RRS1
5w3x_D2e-181462091174Disease resistance protein RRS1
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT2G40750-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: In leaves, level increases gradually up to the point of leaf senescence. {ECO:0000269|PubMed:22268143}.
UniprotTISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:22268143}.
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group III
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:22268143). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). Negative regulator of SA biosynthesis (PubMed:28837631). Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways (PubMed:28837631). {ECO:0000250|UniProtKB:Q9SUP6, ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631, ECO:0000303|PubMed:28837631}.
Function -- GeneRIF ? help Back to Top
  1. WRKY54 and WRKY70 are negative regulators of leaf senescence.
    [PMID: 22268143]
  2. WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling.
    [PMID: 23815736]
  3. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses
    [PMID: 28576847]
  4. data suggest that the elevated SA level in the wrky54wrky70 double mutant results in moderate accumulation of H2O2, in promoting cell wall fortification and consequently enhanced resistance to necrotrophs but is not sufficient to trigger hypersensitive reaction (HR)-like cell death and resistance to biotrophs/hemibiotrophs like Pst DC3000
    [PMID: 28837631]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by reactive oxygen species (ROS) and salicylic acid (SA) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Up-regulated by E.amylovora (PubMed:22316300). {ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:23815736}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT2G24570 (R), AT4G31550 (R)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDsalicylic acid
Interaction ? help Back to Top
Source Intact With
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G40750
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4262530.0AF426253.1 Arabidopsis thaliana WRKY transcription factor 54 (WRKY54) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_181607.10.0WRKY DNA-binding protein 54
SwissprotQ93WU80.0WRK54_ARATH; Probable WRKY transcription factor 54
STRINGAT2G40750.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. S
    ABA activates ADPR cyclase and cADPR induces a subset of ABA-responsive genes in Arabidopsis.
    Plant J., 2004. 38(3): p. 381-95
  4. Scheible WR, et al.
    Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen.
    Plant Physiol., 2004. 136(1): p. 2483-99
  5. Wang Y,Joshi T,Zhang XS,Xu D,Chen L
    Inferring gene regulatory networks from multiple microarray datasets.
    Bioinformatics, 2006. 22(19): p. 2413-20
  6. Wang D,Amornsiripanitch N,Dong X
    A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants.
    PLoS Pathog., 2006. 2(11): p. e123
  7. Journot-Catalino N,Somssich IE,Roby D,Kroj T
    The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana.
    Plant Cell, 2006. 18(11): p. 3289-302
  8. Xin Z,Mandaokar A,Chen J,Last RL,Browse J
    Arabidopsis ESK1 encodes a novel regulator of freezing tolerance.
    Plant J., 2007. 49(5): p. 786-99
  9. Krinke O, et al.
    Phosphatidylinositol 4-kinase activation is an early response to salicylic acid in Arabidopsis suspension cells.
    Plant Physiol., 2007. 144(3): p. 1347-59
  10. Che P,Lall S,Howell SH
    Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture.
    Planta, 2007. 226(5): p. 1183-94
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  12. Charron JB,Ouellet F,Houde M,Sarhan F
    The plant Apolipoprotein D ortholog protects Arabidopsis against oxidative stress.
    BMC Plant Biol., 2008. 8: p. 86

  13. Cysteine homeostasis plays an essential role in plant immunity.
    New Phytol., 2012. 193(1): p. 165-77
  14. Besseau S,Li J,Palva ET
    WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana.
    J. Exp. Bot., 2012. 63(7): p. 2667-79
  15. Moreau M, et al.
    EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora.
    Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30
  16. Li J, et al.
    Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis.
    New Phytol., 2013. 200(2): p. 457-72
  17. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  18. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  19. Lee MH,Jeon HS,Kim HG,Park OK
    An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164.
    New Phytol., 2017. 214(1): p. 343-360
  20. Timmermann T, et al.
    Paraburkholderia phytofirmans PsJN Protects Arabidopsis thaliana Against a Virulent Strain of Pseudomonas syringae Through the Activation of Induced Resistance.
    Mol. Plant Microbe Interact., 2017. 30(3): p. 215-230
  21. Chen J, et al.
    Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses.
    Plant Cell, 2017. 29(6): p. 1425-1439
  22. Li J,Zhong R,Palva ET
    WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis.
    PLoS ONE, 2017. 12(8): p. e0183731