PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G26150.1
Common NameATHSFA2, HSF04, HSFA2, T19L18.4
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 345aa    MW: 39114.1 Da    PI: 4.8984
Description heat shock transcription factor A2
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G26150.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind115.63.1e-36451362102
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k+ye++ed+++++++sws+  nsfvv+d+++f++++Lp+yFkhsnf+SF+RQLn+YgF+k++ ++         weF+++ F +g+k+ll++ik
   AT2G26150.1  45 FLTKTYEMVEDPATDTVVSWSNGRNSFVVWDSHKFSTTLLPRYFKHSNFSSFIRQLNTYGFRKIDPDR---------WEFANEGFLAGQKHLLKNIK 132
                   9****************************************************************999.........******************** PP

                   XXXX CS
  HSF_DNA-bind  99 rkks 102
                   r+++
   AT2G26150.1 133 RRRN 136
                   *986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.10.10.106.1E-3941134IPR011991Winged helix-turn-helix DNA-binding domain
SuperFamilySSF467856.26E-3641134IPR011991Winged helix-turn-helix DNA-binding domain
SMARTSM004151.3E-6041134IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.8E-194568IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004475.1E-3245134IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.8E-198395IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS00434084108IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000562.8E-1996108IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009644Biological Processresponse to high light intensity
GO:0010200Biological Processresponse to chitin
GO:0010286Biological Processheat acclimation
GO:0034605Biological Processcellular response to heat
GO:0034620Biological Processcellular response to unfolded protein
GO:0042542Biological Processresponse to hydrogen peroxide
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0071456Biological Processcellular response to hypoxia
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0016021Cellular Componentintegral component of membrane
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000084anatomyplant sperm cell
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009046anatomyflower
PO:0009052anatomyflower pedicel
PO:0025281anatomypollen
PO:0001185developmental stageplant embryo globular stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 345 aa     Download sequence    Send to blast
MEELKVEMEE ETVTFTGSVA ASSSVGSSSS PRPMEGLNET GPPPFLTKTY EMVEDPATDT  60
VVSWSNGRNS FVVWDSHKFS TTLLPRYFKH SNFSSFIRQL NTYGFRKIDP DRWEFANEGF  120
LAGQKHLLKN IKRRRNMGLQ NVNQQGSGMS CVEVGQYGFD GEVERLKRDH GVLVAEVVRL  180
RQQQHSSKSQ VAAMEQRLLV TEKRQQQMMT FLAKALNNPN FVQQFAVMSK EKKSLFGLDV  240
GRKRRLTSTP SLGTMEENLL HDQEFDRMKD DMEMLFAAAI DDEANNSMPT KEEQCLEAMN  300
VMMRDGNLEA ALDVKVEDLV GSPLDWDSQD LHDMVDQMGF LGSEP
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5d5u_B6e-263613417129Heat shock factor protein 1
5d5v_B6e-263613417129Heat shock factor protein 1
5d5v_D6e-263613417129Heat shock factor protein 1
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1127133LKNIKRR
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible266841_at0.0
Expression AtlasAT2G26150-
AtGenExpressAT2G26150-
ATTED-IIAT2G26150-
Functional Description ? help Back to Top
Source Description
TAIRmember of Heat Stress Transcription Factor (Hsf) family. Involved in response to misfolded protein accumulation in the cytosol. Regulated by alternative splicing and non-sense-mediated decay.
UniProtTranscriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs). {ECO:0000269|PubMed:16649111, ECO:0000269|PubMed:17059409, ECO:0000269|PubMed:17085506}.
Function -- GeneRIF ? help Back to Top
  1. AtHsfA2 has role in linking heat shock with oxidative stress signals in Arabidopsis
    [PMID: 16483133]
  2. The results of this study indicate that HsfA2 is a key regulator in the induction of the defence system under several types of environmental stress.
    [PMID: 17059409]
  3. HsfA2 functions as a heat-inducible transactivator, sustains the expression of Hsp genes, and extends the duration of acquired thermotolerance in Arabidopsis.
    [PMID: 17085506]
  4. HsfA2 protects plants against oxidative damage, organelle dysfunction, and subsequent cell death.
    [PMID: 19342427]
  5. HsfA2 appears to function not only in the triggering of response to environmental stress, but also in the amplification of the signal in the response.
    [PMID: 19352026]
  6. HsfA2 is an important transcription factor that confers anoxia tolerance to Arabidopsis seedlings.
    [PMID: 20089772]
  7. The accumulation of polyubiquitinated proteins causes the induction of HsfA2 expression under oxidative stress.
    [PMID: 20147301]
  8. The phenotype of seedlings overexpressing AtSUMO1 resembled the phenotype of AtHsfA2 knock out seedlings, which were more sensitive than wild type seedlings to repeated heat treatment.
    [PMID: 20521085]
  9. These findings indicated that HsfA1d and HsfA1e not only regulate HsfA2 expression but also function as key regulators of the Hsf signaling network in response to environmental stress.
    [PMID: 21471117]
  10. We have established a previously unrecognized molecular connection between the downregulation of RPS1 expression in chloroplast and the activation of HsfA2-dependent heat-responsive genes in nucleus, which is required for heat tolerance in higher plants.
    [PMID: 22570631]
  11. Data indicate that cngc16 mutant pollen have attenuated expression of several heat-stress response genes, including two heat shock transcription factor genes, HsfA2 and HsfB1.
    [PMID: 23370720]
  12. Self-regulation of HsfA2 expression is through a small truncated HsfA2 isoform encoded by the splice variant.
    [PMID: 23503691]
  13. transcriptional responses of the OPDA-responsive genes HsfA2 and DREB2A to the protein synthesis inhibitor cycloheximide and to the HSP90 inhibitor geldanamycin
    [PMID: 25036962]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G26150.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By heat stress, high light and hydrogen peroxide (H(2)O(2)). {ECO:0000269|PubMed:16649111, ECO:0000269|PubMed:17059409, ECO:0000269|PubMed:17085506}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G32330 (A), AT2G30250 (A), AT3G02990 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G56600(A), AT1G60470(A), AT1G74310(A), AT2G47180(A), AT3G09640(A), AT3G22840(A), AT3G24500(A), AT3G57520(A)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT2G26150, AT4G17750, AT5G16820
IntActSearch O80982
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Heat-sensitive phenotype. {ECO:0000269|PubMed:20521085}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G26150
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK1187440.0AK118744.1 Arabidopsis thaliana At2g26150 mRNA for putative heat shock transcription factor, complete cds, clone: RAFL21-09-G23.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_180184.10.0heat stress transcription factor A-2
SwissprotO809820.0HSFA2_ARATH; Heat stress transcription factor A-2
TrEMBLD7LCR00.0D7LCR0_ARALL; ATHSFA2
STRINGAT2G26150.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP9617233
MalvidsOGEM116722732
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
    [PMID:11910074]
  4. Che P,Gingerich DJ,Lall S,Howell SH
    Global and hormone-induced gene expression changes during shoot development in Arabidopsis.
    Plant Cell, 2002. 14(11): p. 2771-85
    [PMID:12417700]
  5. Port M, et al.
    Role of Hsp17.4-CII as coregulator and cytoplasmic retention factor of tomato heat stress transcription factor HsfA2.
    Plant Physiol., 2004. 135(3): p. 1457-70
    [PMID:15247379]
  6. Busch W,Wunderlich M,Sch
    Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana.
    Plant J., 2005. 41(1): p. 1-14
    [PMID:15610345]
  7. Li C, et al.
    AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis.
    Sci. China, C, Life Sci., 2005. 48(6): p. 540-50
    [PMID:16483133]
  8. Schramm F, et al.
    The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis.
    Plant Mol. Biol., 2006. 60(5): p. 759-72
    [PMID:16649111]
  9. Nishizawa A, et al.
    Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress.
    Plant J., 2006. 48(4): p. 535-47
    [PMID:17059409]
  10. Charng YY, et al.
    A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis.
    Plant Physiol., 2007. 143(1): p. 251-62
    [PMID:17085506]
  11. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  12. Ogawa D,Yamaguchi K,Nishiuchi T
    High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth.
    J. Exp. Bot., 2007. 58(12): p. 3373-83
    [PMID:17890230]
  13. Suzuki N,Bajad S,Shuman J,Shulaev V,Mittler R
    The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana.
    J. Biol. Chem., 2008. 283(14): p. 9269-75
    [PMID:18201973]
  14. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  15. Nishizawa A,Yabuta Y,Shigeoka S
    Galactinol and raffinose constitute a novel function to protect plants from oxidative damage.
    Plant Physiol., 2008. 147(3): p. 1251-63
    [PMID:18502973]
  16. Li S,Fu Q,Huang W,Yu D
    Functional analysis of an Arabidopsis transcription factor WRKY25 in heat stress.
    Plant Cell Rep., 2009. 28(4): p. 683-93
    [PMID:19125253]
  17. Sugio A,Dreos R,Aparicio F,Maule AJ
    The cytosolic protein response as a subcomponent of the wider heat shock response in Arabidopsis.
    Plant Cell, 2009. 21(2): p. 642-54
    [PMID:19244141]
  18. Zhang L,Li Y,Xing D,Gao C
    Characterization of mitochondrial dynamics and subcellular localization of ROS reveal that HsfA2 alleviates oxidative damage caused by heat stress in Arabidopsis.
    J. Exp. Bot., 2009. 60(7): p. 2073-91
    [PMID:19342427]
  19. Nishizawa-Yokoi A,Yoshida E,Yabuta Y,Shigeoka S
    Analysis of the regulation of target genes by an Arabidopsis heat shock transcription factor, HsfA2.
    Biosci. Biotechnol. Biochem., 2009. 73(4): p. 890-5
    [PMID:19352026]
  20. Meiri D,Breiman A
    Arabidopsis ROF1 (FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting the accumulation of HsfA2-regulated sHSPs.
    Plant J., 2009. 59(3): p. 387-99
    [PMID:19366428]
  21. Nishizawa-Yokoi A,Yabuta Y,Shigeoka S
    The contribution of carbohydrates including raffinose family oligosaccharides and sugar alcohols to protection of plant cells from oxidative damage.
    Plant Signal Behav, 2008. 3(11): p. 1016-8
    [PMID:19704439]
  22. Scarpeci TE,Zanor MI,Valle EM
    Investigating the role of plant heat shock proteins during oxidative stress.
    Plant Signal Behav, 2008. 3(10): p. 856-7
    [PMID:19704521]
  23. Meiri D, et al.
    Involvement of Arabidopsis ROF2 (FKBP65) in thermotolerance.
    Plant Mol. Biol., 2010. 72(1-2): p. 191-203
    [PMID:19876748]
  24. Banti V,Mafessoni F,Loreti E,Alpi A,Perata P
    The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis.
    Plant Physiol., 2010. 152(3): p. 1471-83
    [PMID:20089772]
  25. Nishizawa-Yokoi A, et al.
    The 26S proteasome function and Hsp90 activity involved in the regulation of HsfA2 expression in response to oxidative stress.
    Plant Cell Physiol., 2010. 51(3): p. 486-96
    [PMID:20147301]
  26. Hsu SF,Lai HC,Jinn TL
    Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis.
    Plant Physiol., 2010. 153(2): p. 773-84
    [PMID:20388662]
  27. Li M,Berendzen KW,Sch
    Promoter specificity and interactions between early and late Arabidopsis heat shock factors.
    Plant Mol. Biol., 2010. 73(4-5): p. 559-67
    [PMID:20458611]
  28. Cohen-Peer R,Schuster S,Meiri D,Breiman A,Avni A
    Sumoylation of Arabidopsis heat shock factor A2 (HsfA2) modifies its activity during acquired thermotholerance.
    Plant Mol. Biol., 2010. 74(1-2): p. 33-45
    [PMID:20521085]
  29. Yang CY,Hsu FC,Li JP,Wang NN,Shih MC
    The AP2/ERF transcription factor AtERF73/HRE1 modulates ethylene responses during hypoxia in Arabidopsis.
    Plant Physiol., 2011. 156(1): p. 202-12
    [PMID:21398256]
  30. Nishizawa-Yokoi A, et al.
    HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress.
    Plant Cell Physiol., 2011. 52(5): p. 933-45
    [PMID:21471117]
  31. Enoki Y,Sakurai H
    Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms.
    FEBS Lett., 2011. 585(9): p. 1293-8
    [PMID:21510947]
  32. Ikeda M,Mitsuda N,Ohme-Takagi M
    Arabidopsis HsfB1 and HsfB2b act as repressors of the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance.
    Plant Physiol., 2011. 157(3): p. 1243-54
    [PMID:21908690]
  33. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
    [PMID:22037706]
  34. Yu HD, et al.
    Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and its target genes in Arabidopsis.
    PLoS Genet., 2012. 8(5): p. e1002669
    [PMID:22570631]
  35. Mehterov N, et al.
    Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes.
    Plant Physiol. Biochem., 2012. 59: p. 20-9
    [PMID:22710144]
  36. Shahnejat-Bushehri S,Mueller-Roeber B,Balazadeh S
    Arabidopsis NAC transcription factor JUNGBRUNNEN1 affects thermomemory-associated genes and enhances heat stress tolerance in primed and unprimed conditions.
    Plant Signal Behav, 2012. 7(12): p. 1518-21
    [PMID:23073024]
  37. Wang Q,Tu X,Zhang J,Chen X,Rao L
    Heat stress-induced BBX18 negatively regulates the thermotolerance in Arabidopsis.
    Mol. Biol. Rep., 2013. 40(3): p. 2679-88
    [PMID:23238922]
  38. Tunc-Ozdemir M, et al.
    A cyclic nucleotide-gated channel (CNGC16) in pollen is critical for stress tolerance in pollen reproductive development.
    Plant Physiol., 2013. 161(2): p. 1010-20
    [PMID:23370720]
  39. Liu J, et al.
    An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing.
    Plant Physiol., 2013. 162(1): p. 512-21
    [PMID:23503691]
  40. Evrard A, et al.
    Regulation of the heat stress response in Arabidopsis by MPK6-targeted phosphorylation of the heat stress factor HsfA2.
    PeerJ, 2013. 1: p. e59
    [PMID:23638397]
  41. Masuda S,Tokaji Y,Kobayashi Y,Ohta H
    Mechanisms of induction of the stress-responsive transcription factors HsfA2 and DREB2A by 12-oxo-phytodienoic acid in Arabidopsis thaliana.
    Biosci. Biotechnol. Biochem., 2014. 78(4): p. 647-50
    [PMID:25036962]
  42. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  43. Nover L, et al.
    The Hsf world: classification and properties of plant heat stress transcription factors.
    Cell Stress Chaperones, 1996. 1(4): p. 215-23
    [PMID:9222607]