PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G77980.1
Common NameAGL66
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family MIKC_MADS
Protein Properties Length: 332aa    MW: 38051.4 Da    PI: 4.5876
Description AGAMOUS-like 66
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G77980.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1SRF-TF82.42.8e-26958150
                 S---SHHHHHHHHHHHHHHHHHHHHHHHHHHT-EEEEEEE-TTSEEEEEE CS
       SRF-TF  1 krienksnrqvtfskRrngilKKAeELSvLCdaevaviifsstgklyeys 50
                 krien++nrqvtfskRrng++KKA+ELS+LCd+++a+++fs++++l  +s
  AT1G77980.1  9 KRIENTTNRQVTFSKRRNGLIKKAYELSILCDIDIALLMFSPSDRLSLFS 58
                 79******************************************998776 PP

2K-box164.9e-07121187975
        K-box   9 leeakaeslqqelakLkkeienLqreqRhllGedLesLslkeLqqLeqqLekslkkiRskKnellle 75 
                  + +++ e+l++e+ kL++++ + ++e+R++  + +   +++e +  e+qL + l+++ ++++++l +
  AT1G77980.1 121 AINSDVEELEHEVYKLQQQLLMAEEELRKYEPDPIRFTTMEEYETCEKQLMDTLTRVNQRREHILSQ 187
                  5567899*******************************************************99975 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM004329.9E-36160IPR002100Transcription factor, MADS-box
PROSITE profilePS5006628.805161IPR002100Transcription factor, MADS-box
SuperFamilySSF554558.37E-29281IPR002100Transcription factor, MADS-box
CDDcd002659.88E-35275No hitNo description
PROSITE patternPS003500357IPR002100Transcription factor, MADS-box
PRINTSPR004042.0E-24323IPR002100Transcription factor, MADS-box
PfamPF003194.0E-241055IPR002100Transcription factor, MADS-box
PRINTSPR004042.0E-242338IPR002100Transcription factor, MADS-box
PRINTSPR004042.0E-243859IPR002100Transcription factor, MADS-box
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010152Biological Processpollen maturation
GO:0080092Biological Processregulation of pollen tube growth
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 332 aa     Download sequence    Send to blast
MGRVKLEIKR IENTTNRQVT FSKRRNGLIK KAYELSILCD IDIALLMFSP SDRLSLFSGK  60
TRIEDVFSRY INLSDQEREN ALVFPDQSRR PDFQSKEYLL RTLQQLKAEN DIALQLTNPT  120
AINSDVEELE HEVYKLQQQL LMAEEELRKY EPDPIRFTTM EEYETCEKQL MDTLTRVNQR  180
REHILSQDQL SSYEASALQQ QQSMGGPFGN DVVGGWLTEN GPNEAHLFDA SAHSAMYETL  240
LQGSSSSSNQ NNIMGESNVS NHNGDMFQEW AQAYNSTTAH NPSTLFPPMQ HQHGLVVDPN  300
IEEIEIPVMK KDAQADHEVS DYDIRMPQLS SQ
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
6bz1_A7e-18190191MEF2 CHIMERA
6bz1_B7e-18190191MEF2 CHIMERA
6bz1_C7e-18190191MEF2 CHIMERA
6bz1_D7e-18190191MEF2 CHIMERA
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453376950.0
Genevisible262179_at0.0
Expression AtlasAT1G77980-
AtGenExpressAT1G77980-
ATTED-IIAT1G77980-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in pollen. {ECO:0000269|PubMed:12949148}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a member of the MIKC (MADS box, Keratin binding domain, and C terminal domain containing )family of transcriptional regulators. AGL66 is expressed in pollen.It forms heterodimers with other MICK family members (AGL104). Involved in late stages of pollen development and pollen tube growth.
UniProtProbable transcription factor that forms a heterodimer with the MADS-box protein AGL30 and is involved in the regulation of pollen maturation at the late stages of pollen development and pollen tube growth. {ECO:0000269|PubMed:19211705}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G77980.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
IntActSearch Q1PFC2
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No visible phenotype under normal growth conditions. Pollen grains from the double mutant agl66 and agl104 have severly reduced viability, delayed germination and aberrant pollen tube growth. {ECO:0000269|PubMed:19211705}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G77980
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankDQ4464410.0DQ446441.1 Arabidopsis thaliana clone pENTR221-At1g77980 MADS-box family protein (At1g77980) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_177921.20.0AGAMOUS-like 66
SwissprotQ1PFC20.0AGL66_ARATH; Agamous-like MADS-box protein AGL66
TrEMBLA0A178WFT90.0A0A178WFT9_ARATH; AGL66
STRINGAT1G77980.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM21792365
Representative plantOGRP1617761
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. De Bodt S, et al.
    Genomewide structural annotation and evolutionary analysis of the type I MADS-box genes in plants.
    J. Mol. Evol., 2003. 56(5): p. 573-86
    [PMID:12698294]
  3. Parenicová L, et al.
    Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
    Plant Cell, 2003. 15(7): p. 1538-51
    [PMID:12837945]
  4. Kofuji R, et al.
    Evolution and divergence of the MADS-box gene family based on genome-wide expression analyses.
    Mol. Biol. Evol., 2003. 20(12): p. 1963-77
    [PMID:12949148]
  5. de Folter S, et al.
    Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.
    Plant Cell, 2005. 17(5): p. 1424-33
    [PMID:15805477]
  6. Pina C,Pinto F,Feij
    Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation.
    Plant Physiol., 2005. 138(2): p. 744-56
    [PMID:15908605]
  7. Verelst W,Saedler H,Münster T
    MIKC* MADS-protein complexes bind motifs enriched in the proximal region of late pollen-specific Arabidopsis promoters.
    Plant Physiol., 2007. 143(1): p. 447-60
    [PMID:17071640]
  8. Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P
    Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.
    Plant Biotechnol. J., 2006. 4(3): p. 317-24
    [PMID:17147637]
  9. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  10. Adamczyk BJ,Fernandez DE
    MIKC* MADS domain heterodimers are required for pollen maturation and tube growth in Arabidopsis.
    Plant Physiol., 2009. 149(4): p. 1713-23
    [PMID:19211705]
  11. Reňák D,Dupl'áková N,Honys D
    Wide-scale screening of T-DNA lines for transcription factor genes affecting male gametophyte development in Arabidopsis.
    Sex. Plant Reprod., 2012. 25(1): p. 39-60
    [PMID:22101548]
  12. Xia C, et al.
    The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana.
    Plant J., 2014. 79(5): p. 741-56
    [PMID:24923357]
  13. Zhang S, et al.
    FERTILIZATION-INDEPENDENT SEED-Polycomb Repressive Complex 2 Plays a Dual Role in Regulating Type I MADS-Box Genes in Early Endosperm Development.
    Plant Physiol., 2018. 177(1): p. 285-299
    [PMID:29523711]