PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G68840.1
Common NameAtRAV2, EDF2, F14K14.5, RAP2-8, RAP2.8, RAV2, T6L1.3, TEM2
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family RAV
Protein Properties Length: 352aa    MW: 39492.8 Da    PI: 9.8684
Description related to ABI3/VP1 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G68840.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          AP2   1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  s+ykGV   + +grW A+I++      ++r++lg+f   eeAa+ ++ a+ +++g
                  89****9888.8*********3.....5************************998 PP

           B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrsefe 91 
                  f+k+ tpsdv+k++rlv+pk++ae+h           ++++ + +ed +g++W+++++y+++s++yvltkGW++Fvk+++L++gD+v+F+++   e++
                  89*************************9988743.345999*************************************************76554444 PP

                  . CS
           B3  92 l 92 
  AT1G68840.1 285 L 285
                  5 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF541712.09E-1663119IPR016177DNA-binding domain
CDDcd000185.28E-2763121No hitNo description
PfamPF008472.5E-863111IPR001471AP2/ERF domain
PROSITE profilePS5103220.12664119IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.103.3E-2064119IPR001471AP2/ERF domain
SMARTSM003806.2E-3264125IPR001471AP2/ERF domain
Gene3DG3DSA:2.40.330.102.6E-41181293IPR015300DNA-binding pseudobarrel domain
SuperFamilySSF1019363.4E-32185289IPR015300DNA-binding pseudobarrel domain
CDDcd100177.77E-30187278No hitNo description
PfamPF023621.6E-29188288IPR003340B3 DNA binding domain
PROSITE profilePS5086314.184188291IPR003340B3 DNA binding domain
SMARTSM010191.2E-27188293IPR003340B3 DNA binding domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009873Biological Processethylene-activated signaling pathway
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000005anatomycultured plant cell
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000293anatomyguard cell
PO:0009025anatomyvascular leaf
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 352 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wid_A8e-6218529411119DNA-binding protein RAV1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.253440.0leaf| root| seed| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT1G68840-
Functional Description ? help Back to Top
Source Description
TAIRRav2 is part of a complex that has been named `regulator of the (H+)-ATPase of the vacuolar and endosomal membranes' (RAVE)
UniProtProbably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional repressor of flowering time on long day plants. Acts directly on FT expression by binding 5'-CAACA-3' and 5'-CACCTG-3 sequences (Probable). Functionally redundant with TEM1. {ECO:0000250, ECO:0000269|PubMed:18718758, ECO:0000305}.
Function -- GeneRIF ? help Back to Top
  1. TEMPRANILLO genes (TEM1 and TEM2) act as novel direct Flowering locus T repressors.
    [PMID: 18718758]
  2. Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner.
    [PMID: 19420805]
  3. Transgenic cotton (Gossypium hirsutum) expressing AtRAV1/2 and/or AtABI5 showed resistance to imposed drought stress.
    [PMID: 24483851]
  4. Arabidopsis RAV1, RAV2 and Rav1L transcription factors modulates drought and salt stress responses independent of abscisic acid.
    [PMID: 25189341]
  5. GT-4 interacts with TEM2 and then co-regulates the salt responsive gene Cor15A to improve salt stress tolerance.
    [PMID: 25465615]
  6. Data show that FOREVER YOUNG FLOWER UP-REGULATING FACTOR1 (FUF1; At1g71450) acts as an activator in suppressing ETHYLENE RESPONSE DNA BINDING FACTORs EDF1/2/3/4 function and senescence/abscission of the flowers.
    [PMID: 26063506]
  7. TEM2 floral repressor is up-regulated by SVP protein at low temperatures and is involved in genetic pathways that regulate flowering.
    [PMID: 26243615]
  8. AtRAV1 and AtRAV2-overexpressing cotton had approximately 5% significantly longer fibers with only marginal decreases in yields under well-watered or drought stress conditions that resulted in 40-60% yield penalties and 3-7% fiber length penalties in control plants.
    [PMID: 26706061]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G68840
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC0116650.0AC011665.8 Arabidopsis thaliana chromosome 1 BAC T6L1 genomic sequence, complete sequence.
GenBankAC0119140.0AC011914.9 Arabidopsis thaliana chromosome 1 BAC F14K14 genomic sequence, complete sequence.
GenBankAF3603120.0AF360312.1 Arabidopsis thaliana putative DNA-binding protein(RAV2 (At1g68840) mRNA, complete cds.
GenBankAY0563610.0AY056361.1 Arabidopsis thaliana putative DNA-binding protein RAV2 (At1g68840) mRNA, complete cds.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001185352.10.0related to ABI3/VP1 2
RefseqNP_564947.10.0related to ABI3/VP1 2
SwissprotP822800.0RAV2_ARATH; AP2/ERF and B3 domain-containing transcription repressor RAV2
STRINGAT1G68840.20.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP36516108
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Seol JH,Shevchenko A,Shevchenko A,Deshaies RJ
    Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly.
    Nat. Cell Biol., 2001. 3(4): p. 384-91
  3. Dal Bosco C, et al.
    Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana.
    J. Biol. Chem., 2004. 279(2): p. 1060-9
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Stepanova AN,Hoyt JM,Hamilton AA,Alonso JM
    A Link between ethylene and auxin uncovered by the characterization of two root-specific ethylene-insensitive mutants in Arabidopsis.
    Plant Cell, 2005. 17(8): p. 2230-42
  6. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
  7. Castillejo C,Pelaz S
    The balance between CONSTANS and TEMPRANILLO activities determines FT expression to trigger flowering.
    Curr. Biol., 2008. 18(17): p. 1338-43
  8. Swaminathan K,Peterson K,Jack T
    The plant B3 superfamily.
    Trends Plant Sci., 2008. 13(12): p. 647-55
  9. Ikeda M,Ohme-Takagi M
    A novel group of transcriptional repressors in Arabidopsis.
    Plant Cell Physiol., 2009. 50(5): p. 970-5
  10. Kagaya Y,Hattori T
    Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner.
    Genes Genet. Syst., 2009. 84(1): p. 95-9
  11. Chen MK, et al.
    The MADS box gene, FOREVER YOUNG FLOWER, acts as a repressor controlling floral organ senescence and abscission in Arabidopsis.
    Plant J., 2011. 68(1): p. 168-85
  12. Sawa M,Kay SA
    GIGANTEA directly activates Flowering Locus T in Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2011. 108(28): p. 11698-703
  13. Mittal A, et al.
    Related to ABA-Insensitive3(ABI3)/Viviparous1 and AtABI5 transcription factor coexpression in cotton enhances drought stress adaptation.
    Plant Biotechnol. J., 2014. 12(5): p. 578-89
  14. Matías-Hernández L,Aguilar-Jaramillo AE,Marín-González E,Suárez-López P,Pelaz S
    RAV genes: regulation of floral induction and beyond.
    Ann. Bot., 2014. 114(7): p. 1459-70
  15. Fu M,Kang HK,Son SH,Kim SK,Nam KH
    A subset of Arabidopsis RAV transcription factors modulates drought and salt stress responses independent of ABA.
    Plant Cell Physiol., 2014. 55(11): p. 1892-904
  16. Wang XH, et al.
    Trihelix transcription factor GT-4 mediates salt tolerance via interaction with TEM2 in Arabidopsis.
    BMC Plant Biol., 2014. 14: p. 339
  17. Chen WH,Li PF,Chen MK,Lee YI,Yang CH
    FOREVER YOUNG FLOWER Negatively Regulates Ethylene Response DNA-Binding Factors by Activating an Ethylene-Responsive Factor to Control Arabidopsis Floral Organ Senescence and Abscission.
    Plant Physiol., 2015. 168(4): p. 1666-83
  18. Marín-González E, et al.
    SHORT VEGETATIVE PHASE Up-Regulates TEMPRANILLO2 Floral Repressor at Low Ambient Temperatures.
    Plant Physiol., 2015. 169(2): p. 1214-24
  19. Sun YW, et al.
    Attenuation of Histone Methyltransferase KRYPTONITE-mediated transcriptional gene silencing by Geminivirus.
    Sci Rep, 2015. 5: p. 16476
  20. Mittal A,Jiang Y,Ritchie GL,Burke JJ,Rock CD
    AtRAV1 and AtRAV2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering.
    Plant Sci., 2015. 241: p. 78-95
  21. Matías-Hernández L, et al.
    TEMPRANILLO Reveals the Mesophyll as Crucial for Epidermal Trichome Formation.
    Plant Physiol., 2016. 170(3): p. 1624-39
  22. Li P, et al.
    The Arabidopsis UGT87A2, a stress-inducible family 1 glycosyltransferase, is involved in the plant adaptation to abiotic stresses.
    Physiol Plant, 2017. 159(4): p. 416-432
  23. Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD
    The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81
  24. Kagaya Y,Ohmiya K,Hattori T
    RAV1, a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants.
    Nucleic Acids Res., 1999. 27(2): p. 470-8