PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G66470.1
Common NameAtRHD6, BHLH83, EN112, F28G11.9, RHD6
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 298aa    MW: 32088.1 Da    PI: 6.7464
Description ROOT HAIR DEFECTIVE6
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G66470.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH30.66e-10210251955
                  HHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   9 ErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                   ++RR+ri +++  L+el+P+      +K++ +++LekA+ Y+k Lq
  AT1G66470.1 210 AKNRRERISERLKILQELVPNG-----TKVDLVTMLEKAISYVKFLQ 251
                  69******************97.....8******************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:4.10.280.105.7E-12201258IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088815.32201250IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.44E-13202261IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000832.16E-12203255No hitNo description
SMARTSM003535.0E-12207256IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000104.5E-7210251IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0045944Biological Processpositive regulation of transcription from RNA polymerase II promoter
GO:0048766Biological Processroot hair initiation
GO:0005634Cellular Componentnucleus
GO:0005739Cellular Componentmitochondrion
GO:0001046Molecular Functioncore promoter sequence-specific DNA binding
GO:0001228Molecular Functiontranscriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000256anatomyroot hair cell
PO:0000262anatomytrichoblast
PO:0009005anatomyroot
Sequence ? help Back to Top
Protein Sequence    Length: 298 aa     Download sequence    Send to blast
MALVNDHPNE TNYLSKQNSS SSEDLSSPGL DQPDAAYAGG GGGGGSASSS STMNSDHQQH  60
QGFVFYPSGE DHHNSLMDFN GSSFLNFDHH ESFPPPAISC GGSSGGGGFS FLEGNNMSYG  120
FTNWNHQHHM DIISPRSTET PQGQKDWLYS DSTVVTTGSR NESLSPKSAG NKRSHTGEST  180
QPSKKLSSGV TGKTKPKPTT SPKDPQSLAA KNRRERISER LKILQELVPN GTKVDLVTML  240
EKAISYVKFL QVQVKVLATD EFWPAQGGKA PDISQVKDAI DAILSSSQRD RNSNLITN
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible256358_at0.0
Expression AtlasAT1G66470-
AtGenExpressAT1G66470-
ATTED-IIAT1G66470-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively in flowers. {ECO:0000269|PubMed:12679534}.
Function -- GeneRIF ? help Back to Top
  1. data indicate that AtRHD6 (At1g66470) and AtRSL1 (At5g37800) are bHLH transcription factors that are specifically required for the development of root hairs and act downstream of the genes that regulate epidermal pattern formation
    [PMID: 17556585]
  2. Expression of BdRSL class I genes in root hairless Arabidopsis thaliana root hair defective 6 (Atrhd6) Atrsl1 double mutants, devoid of RSL class I function, restores root hair development indicating that the function of these proteins has been conserved. However, neither AtRSL nor BdRSL class I genes is sufficient for root hair development in A. thaliana
    [PMID: 27494519]
  3. S-nitrosoglutathione and auxin restored the root hair phenotype of the hairless root hair defective 6 (rhd6) mutant.
    [PMID: 27880005]
  4. ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation.
    [PMID: 29233944]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G66470.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9C707
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G66470
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY1283100.0AY128310.1 Arabidopsis thaliana unknown protein (At1g66470) mRNA, complete cds.
GenBankBT0063200.0BT006320.1 Arabidopsis thaliana At1g66470 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_176820.10.0ROOT HAIR DEFECTIVE6
SwissprotQ9C7070.0BH083_ARATH; Transcription factor bHLH83
TrEMBLA0A178W4300.0A0A178W430_ARATH; RHD6
STRINGAT1G66470.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM42602854
Representative plantOGRP35321326
Publications ? help Back to Top
  1. Bernhardt C,Tierney ML
    Expression of AtPRP3, a proline-rich structural cell wall protein from Arabidopsis, is regulated by cell-type-specific developmental pathways involved in root hair formation.
    Plant Physiol., 2000. 122(3): p. 705-14
    [PMID:10712533]
  2. Mendoza L,Alvarez-Buylla ER
    Genetic regulation of root hair development in Arabidopsis thaliana: a network model.
    J. Theor. Biol., 2000. 204(3): p. 311-26
    [PMID:10816357]
  3. Bates TR,Lynch JP
    Plant growth and phosphorus accumulation of wild type and two root hair mutants of Arabidopsis thaliana (Brassicaceae).
    Am. J. Bot., 2000. 87(7): p. 958-63
    [PMID:10898772]
  4. Bates TR,Lynch JP
    The efficiency of Arabidopsis thaliana (Brassicaceae) root hairs in phosphorus acquisition.
    Am. J. Bot., 2000. 87(7): p. 964-70
    [PMID:10898773]
  5. Vissenberg K,Fry SC,Verbelen JP
    Root hair initiation is coupled to a highly localized increase of xyloglucan endotransglycosylase action in Arabidopsis roots.
    Plant Physiol., 2001. 127(3): p. 1125-35
    [PMID:11706192]
  6. Masucci JD,Schiefelbein JW
    The rhd6 Mutation of Arabidopsis thaliana Alters Root-Hair Initiation through an Auxin- and Ethylene-Associated Process.
    Plant Physiol., 1994. 106(4): p. 1335-1346
    [PMID:12232412]
  7. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  8. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  9. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  10. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  11. Heazlewood JL, et al.
    Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.
    Plant Cell, 2004. 16(1): p. 241-56
    [PMID:14671022]
  12. Ahn SJ,Shin R,Schachtman DP
    Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K+ uptake.
    Plant Physiol., 2004. 134(3): p. 1135-45
    [PMID:14988478]
  13. Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK
    Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.
    Plant J., 2004. 38(2): p. 366-79
    [PMID:15078338]
  14. Yuen CY,Sedbrook JC,Perrin RM,Carroll KL,Masson PH
    Loss-of-function mutations of ROOT HAIR DEFECTIVE3 suppress root waving, skewing, and epidermal cell file rotation in Arabidopsis.
    Plant Physiol., 2005. 138(2): p. 701-14
    [PMID:15908600]
  15. L
    Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in Arabidopsis thaliana.
    Mol. Plant Microbe Interact., 2007. 20(2): p. 207-17
    [PMID:17313171]
  16. Menand B, et al.
    An ancient mechanism controls the development of cells with a rooting function in land plants.
    Science, 2007. 316(5830): p. 1477-80
    [PMID:17556585]
  17. Ohashi-Ito K,Bergmann DC
    Regulation of the Arabidopsis root vascular initial population by LONESOME HIGHWAY.
    Development, 2007. 134(16): p. 2959-68
    [PMID:17626058]
  18. Singh SK,Fischer U,Singh M,Grebe M,Marchant A
    Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana.
    BMC Plant Biol., 2008. 8: p. 57
    [PMID:18485206]
  19. Contreras-Cornejo HA,Mac
    Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis.
    Plant Physiol., 2009. 149(3): p. 1579-92
    [PMID:19176721]
  20. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  21. Lee YC, et al.
    Growth promotion of Chinese cabbage and Arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin.
    Mol. Plant Microbe Interact., 2011. 24(4): p. 421-31
    [PMID:21375386]
  22. Jang G,Yi K,Pires ND,Menand B,Dolan L
    RSL genes are sufficient for rhizoid system development in early diverging land plants.
    Development, 2011. 138(11): p. 2273-81
    [PMID:21558375]
  23. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  24. Bruex A, et al.
    A gene regulatory network for root epidermis cell differentiation in Arabidopsis.
    PLoS Genet., 2012. 8(1): p. e1002446
    [PMID:22253603]
  25. Coego A, et al.
    The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression.
    Plant J., 2014. 77(6): p. 944-53
    [PMID:24456507]
  26. Lin CY, et al.
    Pathways involved in vanadate-induced root hair formation in Arabidopsis.
    Physiol Plant, 2015. 153(1): p. 137-48
    [PMID:24833217]
  27. Grierson C,Nielsen E,Ketelaarc T,Schiefelbein J
    Root hairs.
    Arabidopsis Book, 2014. 12: p. e0172
    [PMID:24982600]
  28. Kim CM,Dolan L
    ROOT HAIR DEFECTIVE SIX-LIKE Class I Genes Promote Root Hair Development in the Grass Brachypodium distachyon.
    PLoS Genet., 2016. 12(8): p. e1006211
    [PMID:27494519]
  29. Moro CF, et al.
    S-nitrosoglutathione promotes cell wall remodelling, alters the transcriptional profile and induces root hair formation in the hairless root hair defective 6 (rhd6) mutant of Arabidopsis thaliana.
    New Phytol., 2017. 213(4): p. 1771-1786
    [PMID:27880005]
  30. Bahmani R,Kim DG,Kim JA,Hwang S
    The Density and Length of Root Hairs Are Enhanced in Response to Cadmium and Arsenic by Modulating Gene Expressions Involved in Fate Determination and Morphogenesis of Root Hairs in Arabidopsis.
    Front Plant Sci, 2016. 7: p. 1763
    [PMID:27933081]
  31. Liao X,Lovett B,Fang W,St Leger RJ
    Metarhizium robertsii produces indole-3-acetic acid, which promotes root growth in Arabidopsis and enhances virulence to insects.
    Microbiology (Reading, Engl.), 2017. 163(7): p. 980-991
    [PMID:28708056]
  32. Feng Y, et al.
    Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2017. 114(52): p. 13834-13839
    [PMID:29233944]
  33. Masucci JD,Schiefelbein JW
    Hormones act downstream of TTG and GL2 to promote root hair outgrowth during epidermis development in the Arabidopsis root.
    Plant Cell, 1996. 8(9): p. 1505-17
    [PMID:8837505]