PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G63030.2
Common Nameddf2, DREB1E, ERF032, F16M19.20, F16P17.20
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 181aa    MW: 20649.4 Da    PI: 5.3795
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G63030.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          AP2  2 gykGVrwdkkrgrWvAeIrdpsengkr..krfslgkfgtaeeAakaaiaarkkleg 55
                  y+GVr++    +Wv+e+r+p        +r++lg++ ta++Aa+a++ a ++l+g
                 69****988.8******9997.....337*************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000181.74E-252888No hitNo description
PfamPF008475.1E-112878IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.103.5E-282988IPR001471AP2/ERF domain
PROSITE profilePS5103221.8912986IPR001471AP2/ERF domain
SMARTSM003804.5E-242992IPR001471AP2/ERF domain
SuperFamilySSF541711.11E-192988IPR016177DNA-binding domain
PRINTSPR003672.2E-73041IPR001471AP2/ERF domain
PRINTSPR003672.2E-75268IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010371Biological Processregulation of gibberellin biosynthetic process
GO:0016049Biological Processcell growth
GO:0048510Biological Processregulation of timing of transition from vegetative to reproductive phase
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000014anatomyrosette leaf
Sequence ? help Back to Top
Protein Sequence    Length: 181 aa     Download sequence    Send to blast
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT1G63030-
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the DREB subfamily A-1 of ERF/AP2 transcription factor family (DDF2). The protein contains one AP2 domain. There are six members in this subfamily, including CBF1, CBF2, and CBF3. Overexpression of this gene results in the reduction of gibberellic acid biosynthesis. This gene is expressed in all tissues examined, but most abundantly expressed in rosette leaves and stems. Overexpression of DDF1, a putative paralog of this gene, also reduces gibberellic acid biosynthesis and makes the plants more tolerant to high-salinity levels.
UniProtTranscriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold or dehydration-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation. {ECO:0000269|PubMed:11798174}.
Function -- GeneRIF ? help Back to Top
  1. Data indicate that the transcript levels of HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress.
    [PMID: 23825216]
  2. Data indicate that both transcription factors DWARF AND DELAYED FLOWERING1 (DDF1) and DDF2 are under selective constraint.
    [PMID: 26103993]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- Hormone ? help Back to Top
Source Hormone
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G63030
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC0107950.0AC010795.5 Arabidopsis thaliana chromosome 1 BAC F16M19 genomic sequence, complete sequence.
GenBankAY5608410.0AY560841.1 Arabidopsis thaliana putative AP2/EREBP transcription factor (At1g63030) mRNA, complete cds.
GenBankBT0219170.0BT021917.1 Arabidopsis thaliana At1g63030 mRNA, complete cds.
GenBankBT0257420.0BT025742.1 Arabidopsis thaliana At1g63030 mRNA, complete cds.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
GenBankF16P170.0AC011000.3 Sequence of BAC F16P17 from Arabidopsis thaliana chromosome 1, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001077764.11e-132Integrase-type DNA-binding superfamily protein
RefseqNP_176491.11e-132Integrase-type DNA-binding superfamily protein
SwissprotQ9SGJ61e-133DRE1E_ARATH; Dehydration-responsive element-binding protein 1E
TrEMBLD7KU341e-125D7KU34_ARALL; Uncharacterized protein
STRINGAT1G63030.21e-131(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Sakuma Y, et al.
    DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression.
    Biochem. Biophys. Res. Commun., 2002. 290(3): p. 998-1009
  3. Haake V, et al.
    Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis.
    Plant Physiol., 2002. 130(2): p. 639-48
  4. Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM
    A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol., 2003. 132(2): p. 1020-32
  5. Magome H,Yamaguchi S,Hanada A,Kamiya Y,Oda K
    dwarf and delayed-flowering 1, a novel Arabidopsis mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor.
    Plant J., 2004. 37(5): p. 720-9
  6. Ko JH,Han KH,Park S,Yang J
    Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling.
    Plant Physiol., 2004. 135(2): p. 1069-83
  7. Wang Z,Triezenberg SJ,Thomashow MF,Stockinger EJ
    Multiple hydrophobic motifs in Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation.
    Plant Mol. Biol., 2005. 58(4): p. 543-59
  8. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
  9. Xiong Y,Fei SZ
    Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.).
    Planta, 2006. 224(4): p. 878-88
  10. Osuna D, et al.
    Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings.
    Plant J., 2007. 49(3): p. 463-91
  11. Geddy R,Brown GG
    Genes encoding pentatricopeptide repeat (PPR) proteins are not conserved in location in plant genomes and may be subject to diversifying selection.
    BMC Genomics, 2007. 8: p. 130
  12. Welling A,Palva ET
    Involvement of CBF transcription factors in winter hardiness in birch.
    Plant Physiol., 2008. 147(3): p. 1199-211
  13. Hong JP, et al.
    Identification and characterization of transcription factors regulating Arabidopsis HAK5.
    Plant Cell Physiol., 2013. 54(9): p. 1478-90
  14. Lehti-Shiu MD, et al.
    Molecular Evidence for Functional Divergence and Decay of a Transcription Factor Derived from Whole-Genome Duplication in Arabidopsis thaliana.
    Plant Physiol., 2015. 168(4): p. 1717-34