PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G62300.1
Common NameATWRKY6, F19K23.22, WRKY6
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 553aa    MW: 60593.1 Da    PI: 6.2553
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G62300.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY97.68.2e-31312370259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  +Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+
  AT1G62300.1 312 SDGCQWRKYGQKMAKGNPCPRAYYRCTMAtGCPVRKQVQRCAEDRSILITTYEGNHNHP 370
                  7***************************99****************************7 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.807.9E-34296372IPR003657WRKY domain
SuperFamilySSF1182904.58E-28304372IPR003657WRKY domain
PROSITE profilePS5081129.792306372IPR003657WRKY domain
SMARTSM007742.4E-37311371IPR003657WRKY domain
PfamPF031064.7E-27313370IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0016036Biological Processcellular response to phosphate starvation
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0080169Biological Processcellular response to boron-containing substance deprivation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000025anatomyroot tip
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000256anatomyroot hair cell
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0020030anatomycotyledon
PO:0020100anatomyhypocotyl
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001185developmental stageplant embryo globular stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 553 aa     Download sequence    Send to blast
MDRGWSGLTL DSSSLDLLNP NRISHKNHRR FSNPLAMSRI DEEDDQKTRI STNGSEFRFP  60
VSLSGIRDRE DEDFSSGVAG DNDREVPGEV DFFSDKKSRV CREDDEGFRV KKEEQDDRTD  120
VNTGLNLRTT GNTKSDESMI DDGESSEMED KRAKNELVKL QDELKKMTMD NQKLRELLTQ  180
VSNSYTSLQM HLVSLMQQQQ QQNNKVIEAA EKPEETIVPR QFIDLGPTRA VGEAEDVSNS  240
SSEDRTRSGG SSAAERRSNG KRLGREESPE TESNKIQKVN STTPTTFDQT AEATMRKARV  300
SVRARSEAPM ISDGCQWRKY GQKMAKGNPC PRAYYRCTMA TGCPVRKQVQ RCAEDRSILI  360
TTYEGNHNHP LPPAAVAMAS TTTAAANMLL SGSMSSHDGM MNPTNLLARA VLPCSTSMAT  420
ISASAPFPTV TLDLTHSPPP PNGSNPSSSA ATNNNHNSLM QRPQQQQQQM TNLPPGMLPH  480
VIGQALYNQS KFSGLQFSGG SPSTAAFSQS HAVADTITAL TADPNFTAAL AAVISSMING  540
TNHHDGEGNN KNQ
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A2e-22298374176WRKY transcription factor 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.252600.0leaf| root| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible264746_at0.0
Expression AtlasAT1G62300-
AtGenExpressAT1G62300-
ATTED-IIAT1G62300-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Roots, leaves, shoots, flowers and siliques.
Functional Description ? help Back to Top
Source Description
TAIREncodes a transcription factor WRKY6. Regulates Phosphate1 (Pho1) expression in response to low phosphate (Pi) stress.
UniProtTranscription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269|PubMed:12000796, ECO:0000269|PubMed:25733771}.
Function -- GeneRIF ? help Back to Top
  1. Our results demonstrate that WRKY6 and WRKY42 are involved in Arabidopsis responses to low Pi stress by regulation of PHO1 expression.
    [PMID: 19934380]
  2. Results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions.
    [PMID: 20059736]
  3. WRKY6 is an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation.
    [PMID: 23922208]
  4. WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal.WRKY40 and WRKY6 mediate inhibition of root elongation by E-2-hexenal.
    [PMID: 26243404]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00209DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G62300.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269|PubMed:11449049, ECO:0000269|PubMed:11722756}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G62300 (R), AT2G03340 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G17420(A), AT1G45145(A), AT1G62300(R), AT2G14610(A), AT3G23430(R), AT4G04450(R)
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9C519
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G62300
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF3317120.0AF331712.1 Arabidopsis thaliana transcription factor WRKY6 (WRKY6) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564792.10.0WRKY transcription factor 6
SwissprotQ9C5190.0WRKY6_ARATH; WRKY transcription factor 6
TrEMBLC0SV110.0C0SV11_ARATH; Putative uncharacterized protein At1g62300 (Fragment)
STRINGAT1G62300.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM105728107
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Yu D,Chen C,Chen Z
    Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression.
    Plant Cell, 2001. 13(7): p. 1527-40
    [PMID:11449049]
  4. Robatzek S,Somssich IE
    A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes.
    Plant J., 2001. 28(2): p. 123-33
    [PMID:11722756]
  5. Robatzek S,Somssich IE
    Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
    Genes Dev., 2002. 16(9): p. 1139-49
    [PMID:12000796]
  6. Whitham SA, et al.
    Diverse RNA viruses elicit the expression of common sets of genes in susceptible Arabidopsis thaliana plants.
    Plant J., 2003. 33(2): p. 271-83
    [PMID:12535341]
  7. Laloi C,Mestres-Ortega D,Marco Y,Meyer Y,Reichheld JP
    The Arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor.
    Plant Physiol., 2004. 134(3): p. 1006-16
    [PMID:14976236]
  8. Zou X,Seemann JR,Neuman D,Shen QJ
    A WRKY gene from creosote bush encodes an activator of the abscisic acid signaling pathway.
    J. Biol. Chem., 2004. 279(53): p. 55770-9
    [PMID:15504732]
  9. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  10. Ciolkowski I,Wanke D,Birkenbihl RP,Somssich IE
    Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function.
    Plant Mol. Biol., 2008. 68(1-2): p. 81-92
    [PMID:18523729]
  11. Skibbe M,Qu N,Galis I,Baldwin IT
    Induced plant defenses in the natural environment: Nicotiana attenuata WRKY3 and WRKY6 coordinate responses to herbivory.
    Plant Cell, 2008. 20(7): p. 1984-2000
    [PMID:18641266]
  12. Kim CY,Bove J,Assmann SM
    Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.
    New Phytol., 2008. 180(1): p. 57-70
    [PMID:18705666]
  13. Chang IF, et al.
    Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana.
    Proteomics, 2009. 9(11): p. 2967-85
    [PMID:19452453]
  14. Chen YF, et al.
    The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis.
    Plant Cell, 2009. 21(11): p. 3554-66
    [PMID:19934380]
  15. Kasajima I,Ide Y,Yokota Hirai M,Fujiwara T
    WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana.
    Physiol Plant, 2010. 139(1): p. 80-92
    [PMID:20059736]
  16. Shin R,Jez JM,Basra A,Zhang B,Schachtman DP
    14-3-3 proteins fine-tune plant nutrient metabolism.
    FEBS Lett., 2011. 585(1): p. 143-7
    [PMID:21094157]
  17. Zhou X,Jiang Y,Yu D
    WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis.
    Mol. Cells, 2011. 31(4): p. 303-13
    [PMID:21359674]
  18. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
    [PMID:22065421]
  19. Luna E,Bruce TJ,Roberts MR,Flors V,Ton J
    Next-generation systemic acquired resistance.
    Plant Physiol., 2012. 158(2): p. 844-53
    [PMID:22147520]
  20. Vogelmann K, et al.
    Early senescence and cell death in Arabidopsis saul1 mutants involves the PAD4-dependent salicylic acid pathway.
    Plant Physiol., 2012. 159(4): p. 1477-87
    [PMID:22706448]
  21. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  22. Mackov
    Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expression.
    J. Exp. Bot., 2013. 64(10): p. 2805-15
    [PMID:23669573]
  23. Castrillo G, et al.
    WRKY6 transcription factor restricts arsenate uptake and transposon activation in Arabidopsis.
    Plant Cell, 2013. 25(8): p. 2944-57
    [PMID:23922208]
  24. Su T, et al.
    WRKY42 modulates phosphate homeostasis through regulating phosphate translocation and acquisition in Arabidopsis.
    Plant Physiol., 2015. 167(4): p. 1579-91
    [PMID:25733771]
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  26. Mirabella R, et al.
    WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis.
    Plant J., 2015. 83(6): p. 1082-96
    [PMID:26243404]