PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G52150.2
Common NameATHB15, ATHB-15, CNA, ICU4
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HD-ZIP
Protein Properties Length: 837aa    MW: 91829.3 Da    PI: 6.3679
Description HD-ZIP family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G52150.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Homeobox57.72e-181775357
                 --SS--HHHHHHHHHHHHHSSS--HHHHHHHHHHC....TS-HHHHHHHHHHHHHHHHC CS
     Homeobox  3 kRttftkeqleeLeelFeknrypsaeereeLAkkl....gLterqVkvWFqNrRakekk 57
                 k  ++t+eq+e+Le+l++ +++ps  +r++L +++    +++ +q+kvWFqNrR +ek+
  AT1G52150.2 17 KYVRYTPEQVEALERLYHDCPKPSSIRRQQLIRECpilsNIEPKQIKVWFQNRRCREKQ 75
                 5679*****************************************************97 PP

2START1662.7e-521613692205
                  HHHHHHHHHHHHHHHC-TT-EEEEEXCCTTEEEEEEESSS.SCEEEEEEEECCSCHHHHHHHHHCCCGGCT-TT-SEEEEEEEECTT..EEEEEEEEX CS
        START   2 laeeaaqelvkkalaeepgWvkssesengdevlqkfeeskvdsgealrasgvvdmvlallveellddkeqWdetlakaetlevissg..galqlmvae 97 
                  +aee+++e+++ka+ ++  Wv+++ +++g++++ +++ s++++g a+ra+g+v  +++  v+e+++d++ W + ++++e+++v+ ++  g+++l +++
  AT1G52150.2 161 IAEETLAEFLSKATGTAVEWVQMPGMKPGPDSIGIIAISHGCTGVAARACGLVGLEPT-RVAEIVKDRPSWFRECRAVEVMNVLPTAngGTVELLYMQ 257
                  7899******************************************************.7777777777****************9999********* PP

                  XTTXX-SSX.EEEEEEEEEEE.TTS-EEEEEEEEE-TTS--....-TTSEE-EESSEEEEEEEECTCEEEEEEEE-EE--SSXXHHHHHHHHHHHHHH CS
        START  98 lqalsplvp.RdfvfvRyirqlgagdwvivdvSvdseqkppe...sssvvRaellpSgiliepksnghskvtwvehvdlkgrlphwllrslvksglae 191
                  l+a+++l+p Rdf+ +Ry+  l++g++v++++S+ s+q+ p+    +++vRae+l Sg+li+p+++g+s +++v+h+dl++ +++++lr+l++s  + 
  AT1G52150.2 258 LYAPTTLAPpRDFWLLRYTSVLEDGSLVVCERSLKSTQNGPSmplVQNFVRAEMLSSGYLIRPCDGGGSIIHIVDHMDLEACSVPEVLRPLYESPKVL 355
                  *****************************************999999*************************************************** PP

                  HHHHHHHHTXXXXX CS
        START 192 gaktwvatlqrqce 205
                  ++kt++a+l+++++
  AT1G52150.2 356 AQKTTMAALRQLKQ 369
                  **********9986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003891.2E-151480IPR001356Homeobox domain
SuperFamilySSF466899.84E-171680IPR009057Homeodomain-like
CDDcd000861.31E-161777No hitNo description
PfamPF000465.8E-161875IPR001356Homeobox domain
Gene3DG3DSA:1.10.10.603.5E-181975IPR009057Homeodomain-like
PROSITE profilePS5007115.1272076IPR001356Homeobox domain
CDDcd146861.39E-669108No hitNo description
PROSITE profilePS5084824.824151379IPR002913START domain
CDDcd088752.39E-71155371No hitNo description
SuperFamilySSF559615.63E-36160372No hitNo description
Gene3DG3DSA:3.30.530.201.2E-20160346IPR023393START-like domain
SMARTSM002342.6E-36160370IPR002913START domain
PfamPF018527.3E-50161369IPR002913START domain
PfamPF086703.9E-52694836IPR013978MEKHLA
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009965Biological Processleaf morphogenesis
GO:0010014Biological Processmeristem initiation
GO:0010075Biological Processregulation of meristem growth
GO:0010087Biological Processphloem or xylem histogenesis
GO:0030154Biological Processcell differentiation
GO:0048263Biological Processdetermination of dorsal identity
GO:0080060Biological Processintegument development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008289Molecular Functionlipid binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000026anatomyprimary root tip
PO:0000037anatomyshoot apex
PO:0000229anatomyflower meristem
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009015anatomyportion of vascular tissue
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020003anatomyplant ovule
PO:0020022anatomyinner integument
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020124anatomyroot stele
PO:0020137anatomyleaf apex
PO:0020148anatomyshoot apical meristem
PO:0025022anatomycollective leaf structure
PO:0025275anatomyprocambium
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 837 aa     Download sequence    Send to blast
MAMSCKDGKL GCLDNGKYVR YTPEQVEALE RLYHDCPKPS SIRRQQLIRE CPILSNIEPK  60
QIKVWFQNRR CREKQRKEAS RLQAVNRKLT AMNKLLMEEN DRLQKQVSQL VHENSYFRQH  120
TPNPSLPAKD TSCESVVTSG QHQLASQNPQ RDASPAGLLS IAEETLAEFL SKATGTAVEW  180
VQMPGMKPGP DSIGIIAISH GCTGVAARAC GLVGLEPTRV AEIVKDRPSW FRECRAVEVM  240
NVLPTANGGT VELLYMQLYA PTTLAPPRDF WLLRYTSVLE DGSLVVCERS LKSTQNGPSM  300
PLVQNFVRAE MLSSGYLIRP CDGGGSIIHI VDHMDLEACS VPEVLRPLYE SPKVLAQKTT  360
MAALRQLKQI AQEVTQTNSS VNGWGRRPAA LRALSQRLSR GFNEAVNGFT DEGWSVIGDS  420
MDDVTITVNS SPDKLMGLNL TFANGFAPVS NVVLCAKASM LLQNVPPAIL LRFLREHRSE  480
WADNNIDAYL AAAVKVGPCS ARVGGFGGQV ILPLAHTIEH EEFMEVIKLE GLGHSPEDAI  540
VPRDIFLLQL CSGMDENAVG TCAELIFAPI DASFADDAPL LPSGFRIIPL DSAKQEVSSP  600
NRTLDLASAL EIGSAGTKAS TDQSGNSTCA RSVMTIAFEF GIESHMQEHV ASMARQYVRG  660
IISSVQRVAL ALSPSHISSQ VGLRTPLGTP EAQTLARWIC QSYRGYMGVE LLKSNSDGNE  720
SILKNLWHHT DAIICCSMKA LPVFTFANQA GLDMLETTLV ALQDISLEKI FDDNGRKTLC  780
SEFPQIMQQG FACLQGGICL SSMGRPVSYE RAVAWKVLNE EENAHCICFV FINWSFV
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.110110.0flower| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible265060_at0.0
Expression AtlasAT1G52150-
AtGenExpressAT1G52150-
ATTED-IIAT1G52150-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Highly expressed the developing vascular elements and the adaxial portion of cotyledons. Expressed in developing ovules, stamens and carpels. Expressed in procambium and shoot meristem. {ECO:0000269|PubMed:14701930, ECO:0000269|PubMed:15705957}.
Functional Description ? help Back to Top
Source Description
TAIRMember of the class III HD-ZIP protein family. Contains homeodomain and leucine zipper domain. Critical for vascular development and negatively regulates vascular cell differentiation.
UniProtProbable transcription factor involved in the regulation of meristem development to promote lateral organ formation. May regulates procambial and vascular tissue formation or maintenance, and vascular development in inflorescence stems. {ECO:0000269|PubMed:15598805, ECO:0000269|PubMed:15705957, ECO:0000269|PubMed:16617092}.
Function -- GeneRIF ? help Back to Top
  1. Class III HD-Zip AND KANADI genes required for root development [ATHB15]
    [PMID: 15286295]
  2. Regulation of ATHB15 in a mutatnt A. thaliana that affects vascular cell differentiation and inflorescence elongation.
    [PMID: 15773855]
  3. Alteration of the shoot radial pattern in Arabidiopsis thaliana by a gain-of-function allele of the class III HD-Zip gene INCURVATA4 is reported.
    [PMID: 18956325]
  4. An amino acid substitution in the class III HD-ZIP transcription factor ATHB15 enhanced shoot organogenesis.
    [PMID: 21309978]
  5. stem cell specification pathway is normally repressed by the activity of the HD-zip III transcription factors PHABULOSA (PHB), PHAVOLUTA (PHV) and CORONA (CNA).
    [PMID: 26011610]
  6. Transgenic over-expression of a mutant form of AtHB15 that is resistant to miR165-mediated cleavage reversed the stp-2d mutant phenotype to wild-type, indicating that AtHB15 represses secondary wall development in pith.
    [PMID: 26043238]
  7. The branching ovules of the mutant resemble those of some fossil gymnosperms, implicating BEL1 and HD-ZIPIII genes as players in the evolution of the unbranched ovule form in extant angiosperms.
    [PMID: 26700684]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00200DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G52150.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By auxin. Repressed by miR165 and miR166. {ECO:0000269|PubMed:15773855, ECO:0000269|PubMed:16033795, ECO:0000269|PubMed:16617092, ECO:0000269|PubMed:17237362}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G17950(R)
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G52150
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ4394490.0AJ439449.1 Arabidopsis thaliana mRNA for homeodomain-leucine zipper protein (ATHB-15 gene).
GenBankAY0605560.0AY060556.1 Arabidopsis thaliana At1g52150/F5F19_21 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_849795.10.0Homeobox-leucine zipper family protein / lipid-binding START domain-containing protein
SwissprotQ9ZU110.0ATB15_ARATH; Homeobox-leucine zipper protein ATHB-15
TrEMBLB3H4G80.0B3H4G8_ARATH; Homeobox-leucine zipper family protein / lipid-binding START domain-containing protein
STRINGAT1G52150.20.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM20222678
Representative plantOGRP6511671
Publications ? help Back to Top
  1. Serrano-Cartagena J, et al.
    Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis.
    Genetics, 2000. 156(3): p. 1363-77
    [PMID:11063708]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Baima S, et al.
    The arabidopsis ATHB-8 HD-zip protein acts as a differentiation-promoting transcription factor of the vascular meristems.
    Plant Physiol., 2001. 126(2): p. 643-55
    [PMID:11402194]
  4. Ohashi-Ito K,Fukuda H
    HD-zip III homeobox genes that include a novel member, ZeHB-13 (Zinnia)/ATHB-15 (Arabidopsis), are involved in procambium and xylem cell differentiation.
    Plant Cell Physiol., 2003. 44(12): p. 1350-8
    [PMID:14701930]
  5. Hawker NP,Bowman JL
    Roles for Class III HD-Zip and KANADI genes in Arabidopsis root development.
    Plant Physiol., 2004. 135(4): p. 2261-70
    [PMID:15286295]
  6. Prigge MJ, et al.
    Class III homeodomain-leucine zipper gene family members have overlapping, antagonistic, and distinct roles in Arabidopsis development.
    Plant Cell, 2005. 17(1): p. 61-76
    [PMID:15598805]
  7. Green KA,Prigge MJ,Katzman RB,Clark SE
    CORONA, a member of the class III homeodomain leucine zipper gene family in Arabidopsis, regulates stem cell specification and organogenesis.
    Plant Cell, 2005. 17(3): p. 691-704
    [PMID:15705957]
  8. Kim J, et al.
    microRNA-directed cleavage of ATHB15 mRNA regulates vascular development in Arabidopsis inflorescence stems.
    Plant J., 2005. 42(1): p. 84-94
    [PMID:15773855]
  9. Williams L,Grigg SP,Xie M,Christensen S,Fletcher JC
    Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHD-ZIP target genes.
    Development, 2005. 132(16): p. 3657-68
    [PMID:16033795]
  10. Lee JY, et al.
    Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(15): p. 6055-60
    [PMID:16581911]
  11. Ochando I, et al.
    Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis.
    Plant Physiol., 2006. 141(2): p. 607-19
    [PMID:16617092]
  12. Zhou GK,Kubo M,Zhong R,Demura T,Ye ZH
    Overexpression of miR165 affects apical meristem formation, organ polarity establishment and vascular development in Arabidopsis.
    Plant Cell Physiol., 2007. 48(3): p. 391-404
    [PMID:17237362]
  13. Chandler JW,Cole M,Flier A,Grewe B,Werr W
    The AP2 transcription factors DORNROSCHEN and DORNROSCHEN-LIKE redundantly control Arabidopsis embryo patterning via interaction with PHAVOLUTA.
    Development, 2007. 134(9): p. 1653-62
    [PMID:17376809]
  14. Baucher M,El Jaziri M,Vandeputte O
    From primary to secondary growth: origin and development of the vascular system.
    J. Exp. Bot., 2007. 58(13): p. 3485-501
    [PMID:17898423]
  15. Ochando I,González-Reig S,Ripoll JJ,Vera A,Martínez-Laborda A
    Alteration of the shoot radial pattern in Arabidopsis thaliana by a gain-of-function allele of the class III HD-Zip gene INCURVATA4.
    Int. J. Dev. Biol., 2008. 52(7): p. 953-61
    [PMID:18956325]
  16. Kelley DR,Skinner DJ,Gasser CS
    Roles of polarity determinants in ovule development.
    Plant J., 2009. 57(6): p. 1054-64
    [PMID:19054366]
  17. Zhong R,Ye ZH
    Regulation of HD-ZIP III Genes by MicroRNA 165.
    Plant Signal Behav, 2007. 2(5): p. 351-3
    [PMID:19704656]
  18. Hirakawa Y,Kondo Y,Fukuda H
    TDIF peptide signaling regulates vascular stem cell proliferation via the WOX4 homeobox gene in Arabidopsis.
    Plant Cell, 2010. 22(8): p. 2618-29
    [PMID:20729381]
  19. Duclercq J,Assoumou Ndong YP,Guerineau F,Sangwan RS,Catterou M
    Arabidopsis shoot organogenesis is enhanced by an amino acid change in the ATHB15 transcription factor.
    Plant Biol (Stuttg), 2011. 13(2): p. 317-24
    [PMID:21309978]
  20. Yoshimoto K, et al.
    A chemical biology approach reveals an opposite action between thermospermine and auxin in xylem development in Arabidopsis thaliana.
    Plant Cell Physiol., 2012. 53(4): p. 635-45
    [PMID:22345435]
  21. Meinke DW
    A survey of dominant mutations in Arabidopsis thaliana.
    Trends Plant Sci., 2013. 18(2): p. 84-91
    [PMID:22995285]
  22. Frerigmann H,Berger B,Gigolashvili T
    bHLH05 is an interaction partner of MYB51 and a novel regulator of glucosinolate biosynthesis in Arabidopsis.
    Plant Physiol., 2014. 166(1): p. 349-69
    [PMID:25049362]
  23. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  24. Lee C,Clark SE
    A WUSCHEL-Independent Stem Cell Specification Pathway Is Repressed by PHB, PHV and CNA in Arabidopsis.
    PLoS ONE, 2015. 10(5): p. e0126006
    [PMID:26011610]
  25. Du Q, et al.
    Activation of miR165b represses AtHB15 expression and induces pith secondary wall development in Arabidopsis.
    Plant J., 2015. 83(3): p. 388-400
    [PMID:26043238]
  26. Yamada T,Sasaki Y,Hashimoto K,Nakajima K,Gasser CS
    CORONA, PHABULOSA and PHAVOLUTA collaborate with BELL1 to confine WUSCHEL expression to the nucellus in Arabidopsis ovules.
    Development, 2016. 143(3): p. 422-6
    [PMID:26700684]