PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G50640.1
Common NameATERF3, ERF082, ERF3, ERF-3, F11F12.4, F17J6.16
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 225aa    MW: 25216.1 Da    PI: 9.3731
Description ethylene responsive element binding factor 3
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G50640.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP263.16e-202876355
          AP2  3 ykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55
                 ++GVr ++ +gr++AeIrd ++    ++r++lg+f++aeeAa+a++ a+++l+g
  AT1G50640.1 28 FRGVRKRP-WGRFAAEIRDpWK----KARVWLGTFDSAEEAARAYDSAARNLRG 76
                 8*******.**********883....5*************************99 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003802.7E-362790IPR001471AP2/ERF domain
PfamPF008472.5E-132776IPR001471AP2/ERF domain
PROSITE profilePS5103223.3932784IPR001471AP2/ERF domain
CDDcd000186.84E-302786No hitNo description
PRINTSPR003672.3E-102839IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.6E-312884IPR001471AP2/ERF domain
SuperFamilySSF541715.95E-222885IPR016177DNA-binding domain
PRINTSPR003672.3E-105066IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006952Biological Processdefense response
GO:0010105Biological Processnegative regulation of ethylene-activated signaling pathway
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020100anatomyhypocotyl
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007131developmental stageseedling development stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 225 aa     Download sequence    Send to blast
MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA RVWLGTFDSA  60
EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI RNQNQNQVDP FMDHRLFTDH  120
QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM KPATTKRYPR TPPVVPEDCH SDCDSSSSVI  180
DDDDDIASSS RRRNPPFQFD LNFPPLDCVD LFNGADDLHC TDLRL
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5wx9_A3e-2027841472Ethylene-responsive transcription factor ERF096
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.16450.0leaf| root| seed| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO425626510.0
Genevisible261874_at0.0
Expression AtlasAT1G50640-
AtGenExpressAT1G50640-
ATTED-IIAT1G50640-
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-1 of ERF/AP2 transcription factor family (ATERF-3). The protein contains one AP2 domain. There are 15 members in this subfamily including ATERF-3, ATERF-4, ATERF-7, and leafy petiole.
UniProtActs as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs. {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:11487705, ECO:0000269|PubMed:9756931}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00194DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT1G50640.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress. {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDethylene
Interaction ? help Back to Top
Source Intact With
IntActSearch O80339
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G50640
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0081050.0AB008105.1 Arabidopsis thaliana AtERF-3 mRNA for ethylene responsive element binding factor 3, complete cds.
GenBankAC0125610.0AC012561.2 Arabidopsis thaliana chromosome I BAC F11F12 genomic sequence, complete sequence.
GenBankAC0792790.0AC079279.4 Arabidopsis thaliana chromosome 1 BAC F17J6 genomic sequence, complete sequence.
GenBankCP0026840.0CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_175479.11e-165ethylene responsive element binding factor 3
SwissprotO803391e-166ERF82_ARATH; Ethylene-responsive transcription factor 3
TrEMBLC0SV011e-163C0SV01_ARATH; Uncharacterized protein At1g50640 (Fragment)
STRINGAT1G50640.11e-164(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM10281650
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Fujimoto SY,Ohta M,Usui A,Shinshi H,Ohme-Takagi M
    Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression.
    Plant Cell, 2000. 12(3): p. 393-404
    [PMID:10715325]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Ohta M,Matsui K,Hiratsu K,Shinshi H,Ohme-Takagi M
    Repression domains of class II ERF transcriptional repressors share an essential motif for active repression.
    Plant Cell, 2001. 13(8): p. 1959-68
    [PMID:11487705]
  4. Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM
    A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol., 2003. 132(2): p. 1020-32
    [PMID:12805630]
  5. Cluis CP,Mouchel CF,Hardtke CS
    The Arabidopsis transcription factor HY5 integrates light and hormone signaling pathways.
    Plant J., 2004. 38(2): p. 332-47
    [PMID:15078335]
  6. Jeter CR,Tang W,Henaff E,Butterfield T,Roux SJ
    Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis.
    Plant Cell, 2004. 16(10): p. 2652-64
    [PMID:15367717]
  7. Tsukagoshi H,Saijo T,Shibata D,Morikami A,Nakamura K
    Analysis of a sugar response mutant of Arabidopsis identified a novel B3 domain protein that functions as an active transcriptional repressor.
    Plant Physiol., 2005. 138(2): p. 675-85
    [PMID:15894743]
  8. Ramonell K, et al.
    Loss-of-function mutations in chitin responsive genes show increased susceptibility to the powdery mildew pathogen Erysiphe cichoracearum.
    Plant Physiol., 2005. 138(2): p. 1027-36
    [PMID:15923325]
  9. Kim S,Soltis PS,Wall K,Soltis DE
    Phylogeny and domain evolution in the APETALA2-like gene family.
    Mol. Biol. Evol., 2006. 23(1): p. 107-20
    [PMID:16151182]
  10. Wang J, et al.
    Genomewide nonadditive gene regulation in Arabidopsis allotetraploids.
    Genetics, 2006. 172(1): p. 507-17
    [PMID:16172500]
  11. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  12. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  13. Song CP,Galbraith DW
    AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis.
    Plant Mol. Biol., 2006. 60(2): p. 241-57
    [PMID:16429262]
  14. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
    [PMID:16553893]
  15. Jung J, et al.
    The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis.
    Planta, 2007. 225(3): p. 575-88
    [PMID:16937017]
  16. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  17. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
    [PMID:22065421]
  18. Koyama T, et al.
    A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence.
    Plant Physiol., 2013. 162(2): p. 991-1005
    [PMID:23629833]
  19. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  20. Le MH,Cao Y,Zhang XC,Stacey G
    LIK1, a CERK1-interacting kinase, regulates plant immune responses in Arabidopsis.
    PLoS ONE, 2014. 9(7): p. e102245
    [PMID:25036661]
  21. Hao D,Ohme-Takagi M,Sarai A
    Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant.
    J. Biol. Chem., 1998. 273(41): p. 26857-61
    [PMID:9756931]